Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mcrA and icdC are separated by 233 nucleotides icdC and elbA are separated by 102 nucleotides elbA and ycgX are separated by 699 nucleotides
b1159: mcrA - e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI), at 1,209,569 to 1,210,402
mcrA
b4519: icdC - no description, at 1,210,636 to 1,210,800
icdC
b1160: elbA - hypothetical protein (NCBI), at 1,210,903 to 1,211,226
elbA
b1161: ycgX - hypothetical protein (NCBI), at 1,211,926 to 1,212,330
ycgX
Position (kb)
1210
1211
1212 Strain fitness (log2 ratio)
-1
0
1
2 at 1210.309 kb on + strand, within mcrA at 1210.442 kb on - strand at 1210.631 kb on + strand at 1210.631 kb on + strand at 1210.646 kb on + strand at 1210.646 kb on + strand at 1210.646 kb on + strand at 1210.655 kb on + strand, within icdC at 1210.664 kb on + strand, within icdC at 1210.666 kb on + strand, within icdC at 1210.666 kb on + strand, within icdC at 1210.684 kb on + strand, within icdC at 1210.710 kb on + strand, within icdC at 1210.733 kb on + strand, within icdC at 1210.745 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.753 kb on + strand, within icdC at 1210.769 kb on + strand, within icdC at 1210.794 kb on + strand at 1210.812 kb on + strand at 1210.812 kb on + strand at 1210.982 kb on - strand, within elbA at 1210.987 kb on - strand, within elbA
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 1,210,309 + mcrA b1159 0.89 +0.8 1,210,442 - +0.8 1,210,631 + +0.8 1,210,631 + +1.8 1,210,646 + +0.8 1,210,646 + +0.6 1,210,646 + -1.0 1,210,655 + icdC b4519 0.12 +1.8 1,210,664 + icdC b4519 0.17 +0.4 1,210,666 + icdC b4519 0.18 +0.2 1,210,666 + icdC b4519 0.18 +0.7 1,210,684 + icdC b4519 0.29 -0.9 1,210,710 + icdC b4519 0.45 -0.3 1,210,733 + icdC b4519 0.59 -1.0 1,210,745 + icdC b4519 0.66 -1.5 1,210,753 + icdC b4519 0.71 +1.1 1,210,753 + icdC b4519 0.71 +0.0 1,210,753 + icdC b4519 0.71 +0.4 1,210,753 + icdC b4519 0.71 -0.2 1,210,769 + icdC b4519 0.81 +0.3 1,210,794 + +0.5 1,210,812 + +0.8 1,210,812 + -0.6 1,210,982 - elbA b1160 0.24 -0.4 1,210,987 - elbA b1160 0.26 -1.2
Or see this region's nucleotide sequence