Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfD and ymfE are separated by 0 nucleotides ymfE and lit are separated by 457 nucleotides
b1137: ymfD - e14 prophage; predicted SAM-dependent methyltransferase (NCBI), at 1,196,090 to 1,196,755
ymfD
b1138: ymfE - e14 prophage; predicted inner membrane protein (NCBI), at 1,196,756 to 1,197,460
ymfE
b1139: lit - e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI), at 1,197,918 to 1,198,811
lit
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-2
-1
0
1 at 1195.760 kb on + strand at 1195.807 kb on + strand at 1195.888 kb on + strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.895 kb on - strand at 1195.895 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.932 kb on + strand at 1195.932 kb on + strand at 1195.938 kb on - strand at 1195.941 kb on - strand at 1195.944 kb on + strand at 1195.944 kb on + strand at 1195.954 kb on + strand at 1195.957 kb on - strand at 1195.961 kb on - strand at 1195.963 kb on - strand at 1195.972 kb on - strand at 1195.982 kb on - strand at 1195.985 kb on + strand at 1196.004 kb on - strand at 1196.045 kb on + strand at 1196.045 kb on + strand at 1196.054 kb on + strand at 1196.105 kb on - strand at 1196.127 kb on - strand at 1197.326 kb on - strand, within ymfE at 1197.530 kb on - strand at 1197.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 1,195,760 + -0.4 1,195,807 + -0.2 1,195,888 + +0.7 1,195,894 - -0.5 1,195,894 - -0.2 1,195,894 - +1.2 1,195,895 - +0.4 1,195,895 - -0.1 1,195,896 - +0.4 1,195,896 - +0.3 1,195,896 - -0.4 1,195,932 + +0.3 1,195,932 + +0.8 1,195,938 - +0.0 1,195,941 - -0.1 1,195,944 + +0.2 1,195,944 + +0.4 1,195,954 + -0.8 1,195,957 - +0.2 1,195,961 - -0.8 1,195,963 - -0.2 1,195,972 - +0.0 1,195,982 - -0.3 1,195,985 + +0.2 1,196,004 - -2.3 1,196,045 + -0.2 1,196,045 + -0.2 1,196,054 + -0.2 1,196,105 - +1.0 1,196,127 - -0.2 1,197,326 - ymfE b1138 0.81 -0.5 1,197,530 - +0.8 1,197,781 + +0.8
Or see this region's nucleotide sequence