Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS18825 and HGI48_RS18830 are separated by 70 nucleotides HGI48_RS18830 and HGI48_RS18835 are separated by 195 nucleotides HGI48_RS18835 and HGI48_RS18840 are separated by 14 nucleotides HGI48_RS18840 and HGI48_RS18845 overlap by 17 nucleotides HGI48_RS18845 and HGI48_RS18850 are separated by 4 nucleotides
HGI48_RS18825: HGI48_RS18825 - S8 family serine peptidase, at 4,223,031 to 4,224,203
_RS18825
HGI48_RS18830: HGI48_RS18830 - IS3 family transposase, at 4,224,274 to 4,224,438
_RS18830
HGI48_RS18835: HGI48_RS18835 - nitrogen fixation protein NifM, at 4,224,634 to 4,225,419
_RS18835
HGI48_RS18840: HGI48_RS18840 - nitrogen fixation protein NifZ, at 4,225,434 to 4,225,877
_RS18840
HGI48_RS18845: HGI48_RS18845 - nitrogen fixation protein NifW, at 4,225,861 to 4,226,130
_RS18845
HGI48_RS18850: HGI48_RS18850 - homocitrate synthase, at 4,226,135 to 4,227,283
_RS18850
Position (kb)
4224
4225
4226 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4223.664 kb on - strand, within HGI48_RS18825 at 4223.840 kb on - strand, within HGI48_RS18825 at 4223.977 kb on + strand, within HGI48_RS18825 at 4224.026 kb on - strand, within HGI48_RS18825 at 4224.061 kb on + strand, within HGI48_RS18825 at 4224.084 kb on - strand, within HGI48_RS18825 at 4224.084 kb on - strand, within HGI48_RS18825 at 4224.097 kb on + strand at 4224.098 kb on - strand at 4224.190 kb on + strand at 4224.202 kb on - strand at 4224.214 kb on - strand at 4224.258 kb on + strand at 4224.362 kb on + strand, within HGI48_RS18830 at 4224.362 kb on + strand, within HGI48_RS18830 at 4224.363 kb on - strand, within HGI48_RS18830 at 4224.366 kb on + strand, within HGI48_RS18830 at 4224.366 kb on + strand, within HGI48_RS18830 at 4224.367 kb on - strand, within HGI48_RS18830 at 4224.385 kb on + strand, within HGI48_RS18830 at 4224.417 kb on + strand, within HGI48_RS18830 at 4224.418 kb on - strand at 4224.424 kb on + strand at 4224.452 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.519 kb on + strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.520 kb on - strand at 4224.568 kb on + strand at 4224.590 kb on + strand at 4224.591 kb on - strand at 4224.591 kb on - strand at 4224.636 kb on - strand at 4224.636 kb on - strand at 4224.690 kb on - strand at 4224.690 kb on - strand at 4224.784 kb on + strand, within HGI48_RS18835 at 4225.342 kb on + strand at 4225.342 kb on + strand at 4225.343 kb on - strand at 4225.343 kb on - strand at 4225.456 kb on + strand at 4225.456 kb on + strand at 4225.457 kb on - strand at 4225.457 kb on - strand at 4225.457 kb on - strand at 4225.505 kb on + strand, within HGI48_RS18840 at 4225.605 kb on - strand, within HGI48_RS18840 at 4225.605 kb on - strand, within HGI48_RS18840 at 4225.622 kb on - strand, within HGI48_RS18840 at 4225.717 kb on + strand, within HGI48_RS18840 at 4225.717 kb on + strand, within HGI48_RS18840 at 4225.718 kb on - strand, within HGI48_RS18840 at 4225.718 kb on - strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.721 kb on + strand, within HGI48_RS18840 at 4225.722 kb on - strand at 4225.759 kb on + strand, within HGI48_RS18840 at 4225.759 kb on + strand, within HGI48_RS18840 at 4225.760 kb on - strand at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.760 kb on - strand, within HGI48_RS18840 at 4225.904 kb on + strand, within HGI48_RS18845 at 4225.905 kb on - strand, within HGI48_RS18845 at 4226.051 kb on + strand, within HGI48_RS18845 at 4226.052 kb on - strand, within HGI48_RS18845 at 4226.117 kb on + strand at 4226.118 kb on - strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.184 kb on + strand at 4226.185 kb on - strand at 4226.185 kb on - strand at 4226.185 kb on - strand at 4226.388 kb on + strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850 at 4226.389 kb on - strand, within HGI48_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 4,223,664 - HGI48_RS18825 0.54 -1.2 4,223,840 - HGI48_RS18825 0.69 -1.8 4,223,977 + HGI48_RS18825 0.81 -1.5 4,224,026 - HGI48_RS18825 0.85 +0.1 4,224,061 + HGI48_RS18825 0.88 -0.2 4,224,084 - HGI48_RS18825 0.90 -0.5 4,224,084 - HGI48_RS18825 0.90 -0.6 4,224,097 + -2.0 4,224,098 - +0.8 4,224,190 + +1.0 4,224,202 - -2.5 4,224,214 - -1.0 4,224,258 + +1.8 4,224,362 + HGI48_RS18830 0.53 -0.5 4,224,362 + HGI48_RS18830 0.53 +0.6 4,224,363 - HGI48_RS18830 0.54 +2.6 4,224,366 + HGI48_RS18830 0.56 -0.4 4,224,366 + HGI48_RS18830 0.56 -1.9 4,224,367 - HGI48_RS18830 0.56 -0.7 4,224,385 + HGI48_RS18830 0.67 +1.2 4,224,417 + HGI48_RS18830 0.87 -0.4 4,224,418 - +0.8 4,224,424 + -1.5 4,224,452 + +0.3 4,224,519 + -0.4 4,224,519 + +1.8 4,224,519 + +1.4 4,224,519 + +1.0 4,224,520 - +0.4 4,224,520 - -0.7 4,224,520 - -0.2 4,224,520 - +0.8 4,224,568 + -1.2 4,224,590 + -0.3 4,224,591 - -0.2 4,224,591 - +0.2 4,224,636 - -1.4 4,224,636 - -0.4 4,224,690 - +1.3 4,224,690 - -0.6 4,224,784 + HGI48_RS18835 0.19 +0.4 4,225,342 + +1.0 4,225,342 + -0.0 4,225,343 - -1.0 4,225,343 - -0.5 4,225,456 + -1.0 4,225,456 + -0.5 4,225,457 - +0.2 4,225,457 - -0.3 4,225,457 - -0.2 4,225,505 + HGI48_RS18840 0.16 -0.5 4,225,605 - HGI48_RS18840 0.39 -0.9 4,225,605 - HGI48_RS18840 0.39 -1.1 4,225,622 - HGI48_RS18840 0.42 -0.2 4,225,717 + HGI48_RS18840 0.64 +0.4 4,225,717 + HGI48_RS18840 0.64 +1.8 4,225,718 - HGI48_RS18840 0.64 -0.4 4,225,718 - HGI48_RS18840 0.64 +0.5 4,225,721 + HGI48_RS18840 0.65 -1.7 4,225,721 + HGI48_RS18840 0.65 +1.2 4,225,721 + HGI48_RS18840 0.65 +0.8 4,225,722 - +0.6 4,225,759 + HGI48_RS18840 0.73 -0.4 4,225,759 + HGI48_RS18840 0.73 +0.2 4,225,760 - +0.3 4,225,760 - HGI48_RS18840 0.73 -0.5 4,225,760 - HGI48_RS18840 0.73 -0.5 4,225,760 - HGI48_RS18840 0.73 +0.7 4,225,760 - HGI48_RS18840 0.73 +0.6 4,225,904 + HGI48_RS18845 0.16 -0.1 4,225,905 - HGI48_RS18845 0.16 -1.2 4,226,051 + HGI48_RS18845 0.70 +0.9 4,226,052 - HGI48_RS18845 0.71 -0.6 4,226,117 + -1.2 4,226,118 - -0.5 4,226,184 + +1.3 4,226,184 + -0.2 4,226,184 + +0.5 4,226,184 + +0.5 4,226,184 + +0.8 4,226,185 - +1.0 4,226,185 - -1.7 4,226,185 - -0.7 4,226,388 + HGI48_RS18850 0.22 -0.1 4,226,389 - HGI48_RS18850 0.22 -0.4 4,226,389 - HGI48_RS18850 0.22 -0.1 4,226,389 - HGI48_RS18850 0.22 -1.6 4,226,389 - HGI48_RS18850 0.22 -0.6 4,226,389 - HGI48_RS18850 0.22 +0.1
Or see this region's nucleotide sequence