Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS12490 and HGI48_RS12495 are separated by 63 nucleotides HGI48_RS12495 and HGI48_RS12500 are separated by 220 nucleotides HGI48_RS12500 and HGI48_RS12505 are separated by 87 nucleotides
HGI48_RS12490: HGI48_RS12490 - envelope stress response membrane protein PspB, at 2,731,976 to 2,732,206
_RS12490
HGI48_RS12495: HGI48_RS12495 - phage shock protein PspA, at 2,732,270 to 2,732,935
_RS12495
HGI48_RS12500: HGI48_RS12500 - phage shock protein operon transcriptional activator, at 2,733,156 to 2,734,187
_RS12500
HGI48_RS12505: HGI48_RS12505 - peptide ABC transporter substrate-binding protein SapA, at 2,734,275 to 2,735,987
_RS12505
Position (kb)
2733
2734
2735 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2732.217 kb on + strand at 2732.218 kb on - strand at 2732.221 kb on + strand at 2732.285 kb on + strand at 2732.285 kb on + strand at 2732.286 kb on - strand at 2732.554 kb on + strand, within HGI48_RS12495 at 2732.554 kb on + strand, within HGI48_RS12495 at 2732.800 kb on + strand, within HGI48_RS12495 at 2732.800 kb on + strand, within HGI48_RS12495 at 2732.801 kb on - strand at 2732.821 kb on + strand, within HGI48_RS12495 at 2732.821 kb on + strand, within HGI48_RS12495 at 2732.822 kb on - strand, within HGI48_RS12495 at 2732.822 kb on - strand at 2732.930 kb on - strand at 2732.930 kb on - strand at 2732.935 kb on + strand at 2732.935 kb on + strand at 2732.941 kb on - strand at 2733.017 kb on + strand at 2733.018 kb on - strand at 2733.129 kb on - strand at 2733.178 kb on + strand at 2733.306 kb on + strand, within HGI48_RS12500 at 2733.307 kb on - strand, within HGI48_RS12500 at 2733.410 kb on + strand, within HGI48_RS12500 at 2733.813 kb on + strand, within HGI48_RS12500 at 2733.813 kb on + strand, within HGI48_RS12500 at 2733.814 kb on - strand, within HGI48_RS12500 at 2734.013 kb on + strand, within HGI48_RS12500 at 2734.013 kb on + strand, within HGI48_RS12500 at 2734.013 kb on + strand, within HGI48_RS12500 at 2734.014 kb on - strand, within HGI48_RS12500 at 2734.098 kb on + strand at 2734.098 kb on + strand at 2734.099 kb on - strand at 2734.099 kb on - strand at 2734.201 kb on + strand at 2734.202 kb on - strand at 2734.208 kb on - strand at 2734.208 kb on - strand at 2734.246 kb on + strand at 2734.246 kb on + strand at 2734.246 kb on + strand at 2734.246 kb on + strand at 2734.246 kb on + strand at 2734.246 kb on + strand at 2734.248 kb on + strand at 2734.393 kb on - strand at 2734.408 kb on + strand at 2734.409 kb on - strand at 2734.409 kb on - strand at 2734.750 kb on + strand at 2734.750 kb on + strand at 2734.751 kb on - strand, within HGI48_RS12505 at 2734.863 kb on + strand, within HGI48_RS12505 at 2734.947 kb on + strand, within HGI48_RS12505 at 2734.962 kb on + strand, within HGI48_RS12505 at 2734.962 kb on + strand, within HGI48_RS12505 at 2734.962 kb on + strand, within HGI48_RS12505 at 2734.962 kb on + strand, within HGI48_RS12505 at 2734.963 kb on - strand, within HGI48_RS12505 at 2735.175 kb on + strand, within HGI48_RS12505
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 2,732,217 + -0.3 2,732,218 - -1.3 2,732,221 + +0.9 2,732,285 + -0.7 2,732,285 + -0.5 2,732,286 - -2.1 2,732,554 + HGI48_RS12495 0.43 -2.1 2,732,554 + HGI48_RS12495 0.43 +1.8 2,732,800 + HGI48_RS12495 0.80 -0.1 2,732,800 + HGI48_RS12495 0.80 +0.9 2,732,801 - +0.3 2,732,821 + HGI48_RS12495 0.83 -1.4 2,732,821 + HGI48_RS12495 0.83 +0.5 2,732,822 - HGI48_RS12495 0.83 -1.5 2,732,822 - -1.5 2,732,930 - -1.0 2,732,930 - -2.4 2,732,935 + +0.5 2,732,935 + -1.5 2,732,941 - -0.3 2,733,017 + -0.8 2,733,018 - -1.7 2,733,129 - -1.3 2,733,178 + -0.1 2,733,306 + HGI48_RS12500 0.15 -1.3 2,733,307 - HGI48_RS12500 0.15 +0.3 2,733,410 + HGI48_RS12500 0.25 -1.0 2,733,813 + HGI48_RS12500 0.64 -2.0 2,733,813 + HGI48_RS12500 0.64 -0.5 2,733,814 - HGI48_RS12500 0.64 -0.2 2,734,013 + HGI48_RS12500 0.83 -0.1 2,734,013 + HGI48_RS12500 0.83 -1.5 2,734,013 + HGI48_RS12500 0.83 +1.3 2,734,014 - HGI48_RS12500 0.83 -0.8 2,734,098 + +0.2 2,734,098 + -0.3 2,734,099 - +0.3 2,734,099 - +1.3 2,734,201 + +0.6 2,734,202 - +0.3 2,734,208 - -0.5 2,734,208 - -1.2 2,734,246 + +0.7 2,734,246 + -1.3 2,734,246 + -1.9 2,734,246 + -0.4 2,734,246 + -0.8 2,734,246 + -1.3 2,734,248 + -0.7 2,734,393 - -1.7 2,734,408 + +0.0 2,734,409 - +1.3 2,734,409 - -1.3 2,734,750 + -2.8 2,734,750 + -0.3 2,734,751 - HGI48_RS12505 0.28 -3.4 2,734,863 + HGI48_RS12505 0.34 +0.3 2,734,947 + HGI48_RS12505 0.39 -0.5 2,734,962 + HGI48_RS12505 0.40 -1.3 2,734,962 + HGI48_RS12505 0.40 +1.1 2,734,962 + HGI48_RS12505 0.40 +0.7 2,734,962 + HGI48_RS12505 0.40 +0.2 2,734,963 - HGI48_RS12505 0.40 +0.6 2,735,175 + HGI48_RS12505 0.53 +0.0
Or see this region's nucleotide sequence