Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS12055 and HGI48_RS12060 are separated by 19 nucleotides HGI48_RS12060 and HGI48_RS12065 are separated by 84 nucleotides HGI48_RS12065 and HGI48_RS12070 are separated by 299 nucleotides
HGI48_RS12055: HGI48_RS12055 - carboxy terminal-processing peptidase, at 2,632,115 to 2,634,136
_RS12055
HGI48_RS12060: HGI48_RS12060 - RNA chaperone ProQ, at 2,634,156 to 2,634,887
_RS12060
HGI48_RS12065: HGI48_RS12065 - GAF domain-containing protein, at 2,634,972 to 2,635,478
_RS12065
HGI48_RS12070: HGI48_RS12070 - membrane integrity lipid transport subunit YebS, at 2,635,778 to 2,637,016
_RS12070
Position (kb)
2634
2635 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2633.311 kb on + strand, within HGI48_RS12055 at 2633.312 kb on - strand, within HGI48_RS12055 at 2633.367 kb on + strand, within HGI48_RS12055 at 2633.367 kb on + strand, within HGI48_RS12055 at 2633.368 kb on - strand, within HGI48_RS12055 at 2633.625 kb on + strand at 2633.625 kb on + strand, within HGI48_RS12055 at 2634.036 kb on - strand at 2634.064 kb on + strand at 2634.309 kb on - strand, within HGI48_RS12060 at 2634.601 kb on - strand, within HGI48_RS12060 at 2634.696 kb on - strand, within HGI48_RS12060 at 2634.944 kb on + strand at 2634.945 kb on - strand at 2634.946 kb on + strand at 2634.946 kb on + strand at 2634.946 kb on + strand at 2634.947 kb on - strand at 2634.968 kb on + strand at 2634.968 kb on + strand at 2634.969 kb on - strand at 2634.989 kb on + strand at 2634.989 kb on + strand at 2634.989 kb on + strand at 2635.038 kb on + strand, within HGI48_RS12065 at 2635.116 kb on + strand at 2635.155 kb on + strand, within HGI48_RS12065 at 2635.155 kb on + strand, within HGI48_RS12065 at 2635.155 kb on + strand, within HGI48_RS12065 at 2635.156 kb on - strand, within HGI48_RS12065 at 2635.156 kb on - strand, within HGI48_RS12065 at 2635.156 kb on - strand, within HGI48_RS12065 at 2635.156 kb on - strand, within HGI48_RS12065 at 2635.156 kb on - strand, within HGI48_RS12065 at 2635.275 kb on - strand, within HGI48_RS12065 at 2635.336 kb on + strand, within HGI48_RS12065 at 2635.447 kb on - strand at 2635.447 kb on - strand at 2635.505 kb on + strand at 2635.540 kb on + strand at 2635.540 kb on + strand at 2635.541 kb on - strand at 2635.543 kb on + strand at 2635.543 kb on + strand at 2635.564 kb on + strand at 2635.626 kb on + strand at 2635.626 kb on + strand at 2635.734 kb on + strand at 2635.734 kb on + strand at 2635.735 kb on - strand at 2635.735 kb on - strand at 2635.735 kb on - strand at 2635.739 kb on + strand at 2635.739 kb on + strand at 2635.740 kb on - strand at 2635.740 kb on - strand at 2635.747 kb on + strand at 2635.747 kb on + strand at 2635.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 2,633,311 + HGI48_RS12055 0.59 -3.0 2,633,312 - HGI48_RS12055 0.59 -1.2 2,633,367 + HGI48_RS12055 0.62 -0.4 2,633,367 + HGI48_RS12055 0.62 +0.8 2,633,368 - HGI48_RS12055 0.62 -0.8 2,633,625 + -2.0 2,633,625 + HGI48_RS12055 0.75 -0.2 2,634,036 - -1.6 2,634,064 + -5.4 2,634,309 - HGI48_RS12060 0.21 -0.2 2,634,601 - HGI48_RS12060 0.61 -1.9 2,634,696 - HGI48_RS12060 0.74 -1.5 2,634,944 + +0.1 2,634,945 - -0.4 2,634,946 + +1.7 2,634,946 + +2.3 2,634,946 + +1.0 2,634,947 - -1.2 2,634,968 + +1.2 2,634,968 + +3.0 2,634,969 - +1.4 2,634,989 + +0.9 2,634,989 + +2.0 2,634,989 + +0.8 2,635,038 + HGI48_RS12065 0.13 +2.1 2,635,116 + +2.2 2,635,155 + HGI48_RS12065 0.36 +1.1 2,635,155 + HGI48_RS12065 0.36 +0.4 2,635,155 + HGI48_RS12065 0.36 +0.4 2,635,156 - HGI48_RS12065 0.36 -0.4 2,635,156 - HGI48_RS12065 0.36 -0.0 2,635,156 - HGI48_RS12065 0.36 -3.4 2,635,156 - HGI48_RS12065 0.36 -0.2 2,635,156 - HGI48_RS12065 0.36 -0.2 2,635,275 - HGI48_RS12065 0.60 +0.4 2,635,336 + HGI48_RS12065 0.72 +0.8 2,635,447 - -1.2 2,635,447 - -1.3 2,635,505 + -2.3 2,635,540 + +0.5 2,635,540 + +1.8 2,635,541 - -0.6 2,635,543 + +1.6 2,635,543 + -0.5 2,635,564 + +1.6 2,635,626 + +1.6 2,635,626 + -2.3 2,635,734 + -0.6 2,635,734 + -0.3 2,635,735 - -1.8 2,635,735 - -0.6 2,635,735 - -2.2 2,635,739 + -1.8 2,635,739 + -1.1 2,635,740 - -0.9 2,635,740 - -1.2 2,635,747 + -0.5 2,635,747 + -1.2 2,635,748 - -0.7
Or see this region's nucleotide sequence