Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS04215 and HGI48_RS04220 are separated by 138 nucleotides HGI48_RS04220 and HGI48_RS04225 are separated by 694 nucleotides HGI48_RS04225 and HGI48_RS04230 are separated by 584 nucleotides
HGI48_RS04215: HGI48_RS04215 - nucleotidyl transferase AbiEii/AbiGii toxin family protein, at 887,297 to 888,214
_RS04215
HGI48_RS04220: HGI48_RS04220 - transposase, at 888,353 to 888,501
_RS04220
HGI48_RS04225: HGI48_RS04225 - hypothetical protein, at 889,196 to 889,498
_RS04225
HGI48_RS04230: HGI48_RS04230 - dienelactone hydrolase family protein, at 890,083 to 891,261
_RS04230
Position (kb) 889
890 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 888.231 kb on - strand at 888.262 kb on + strand at 888.262 kb on + strand at 888.263 kb on - strand at 888.304 kb on - strand at 888.309 kb on - strand at 888.347 kb on + strand at 888.348 kb on - strand at 888.348 kb on - strand at 888.369 kb on - strand, within HGI48_RS04220 at 888.369 kb on - strand, within HGI48_RS04220 at 888.370 kb on + strand, within HGI48_RS04220 at 888.370 kb on + strand, within HGI48_RS04220 at 888.467 kb on + strand, within HGI48_RS04220 at 888.474 kb on - strand, within HGI48_RS04220 at 888.552 kb on + strand at 888.553 kb on - strand at 888.612 kb on - strand at 888.636 kb on - strand at 888.781 kb on - strand at 888.790 kb on - strand at 888.819 kb on + strand at 888.838 kb on - strand at 888.991 kb on + strand at 889.014 kb on + strand at 889.017 kb on + strand at 889.030 kb on + strand at 889.040 kb on + strand at 889.066 kb on - strand at 889.183 kb on + strand at 889.246 kb on + strand, within HGI48_RS04225 at 889.253 kb on + strand, within HGI48_RS04225 at 889.254 kb on - strand at 889.254 kb on - strand, within HGI48_RS04225 at 889.254 kb on - strand, within HGI48_RS04225 at 889.254 kb on - strand, within HGI48_RS04225 at 889.322 kb on - strand at 889.418 kb on + strand at 889.419 kb on - strand, within HGI48_RS04225 at 889.448 kb on - strand, within HGI48_RS04225 at 889.468 kb on + strand at 889.503 kb on + strand at 889.504 kb on - strand at 889.533 kb on - strand at 889.535 kb on + strand at 889.614 kb on + strand at 889.622 kb on - strand at 889.770 kb on - strand at 889.873 kb on + strand at 889.979 kb on - strand at 889.999 kb on + strand at 889.999 kb on + strand at 889.999 kb on + strand at 890.004 kb on + strand at 890.004 kb on + strand at 890.004 kb on + strand at 890.005 kb on - strand at 890.007 kb on + strand at 890.010 kb on + strand at 890.010 kb on + strand at 890.011 kb on - strand at 890.011 kb on - strand at 890.011 kb on - strand at 890.013 kb on + strand at 890.014 kb on - strand at 890.050 kb on + strand at 890.055 kb on + strand at 890.055 kb on + strand at 890.114 kb on + strand at 890.114 kb on + strand at 890.114 kb on + strand at 890.114 kb on + strand at 890.115 kb on - strand at 890.123 kb on + strand at 890.350 kb on - strand, within HGI48_RS04230 at 890.350 kb on - strand, within HGI48_RS04230 at 890.391 kb on + strand, within HGI48_RS04230 at 890.392 kb on - strand, within HGI48_RS04230 at 890.392 kb on - strand, within HGI48_RS04230
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 888,231 - -2.4 888,262 + -0.8 888,262 + +0.0 888,263 - -0.1 888,304 - -1.4 888,309 - -1.6 888,347 + +0.8 888,348 - -0.2 888,348 - +0.2 888,369 - HGI48_RS04220 0.11 +0.9 888,369 - HGI48_RS04220 0.11 -0.4 888,370 + HGI48_RS04220 0.11 -1.0 888,370 + HGI48_RS04220 0.11 +0.8 888,467 + HGI48_RS04220 0.77 -1.4 888,474 - HGI48_RS04220 0.81 -0.7 888,552 + -1.4 888,553 - +1.2 888,612 - -0.9 888,636 - -0.6 888,781 - +0.3 888,790 - -1.4 888,819 + -1.2 888,838 - -1.4 888,991 + -0.1 889,014 + -1.8 889,017 + +0.0 889,030 + -0.1 889,040 + -0.6 889,066 - -1.2 889,183 + -0.6 889,246 + HGI48_RS04225 0.17 +2.6 889,253 + HGI48_RS04225 0.19 -2.2 889,254 - -1.4 889,254 - HGI48_RS04225 0.19 -0.8 889,254 - HGI48_RS04225 0.19 -1.3 889,254 - HGI48_RS04225 0.19 -0.4 889,322 - +1.9 889,418 + -1.7 889,419 - HGI48_RS04225 0.74 +1.4 889,448 - HGI48_RS04225 0.83 +0.2 889,468 + -0.2 889,503 + -0.4 889,504 - -1.4 889,533 - -0.1 889,535 + +1.2 889,614 + -0.0 889,622 - -0.7 889,770 - +0.8 889,873 + -0.8 889,979 - +0.9 889,999 + -0.7 889,999 + -1.2 889,999 + +0.2 890,004 + +0.8 890,004 + -1.8 890,004 + +3.2 890,005 - -0.8 890,007 + +2.7 890,010 + +0.3 890,010 + +1.5 890,011 - -2.2 890,011 - +2.2 890,011 - +0.2 890,013 + -1.3 890,014 - +0.3 890,050 + -0.8 890,055 + -0.1 890,055 + -0.2 890,114 + -3.0 890,114 + +0.7 890,114 + -0.7 890,114 + -1.3 890,115 - +1.8 890,123 + -0.4 890,350 - HGI48_RS04230 0.23 -2.5 890,350 - HGI48_RS04230 0.23 +1.2 890,391 + HGI48_RS04230 0.26 -0.4 890,392 - HGI48_RS04230 0.26 +0.2 890,392 - HGI48_RS04230 0.26 -0.1
Or see this region's nucleotide sequence