Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS15555 and HGI48_RS15560 are separated by 140 nucleotides HGI48_RS15560 and HGI48_RS15565 are separated by 10 nucleotides HGI48_RS15565 and HGI48_RS15570 are separated by 13 nucleotides
HGI48_RS15555: HGI48_RS15555 - ribosome biogenesis GTPase Der, at 3,497,212 to 3,498,699
_RS15555
HGI48_RS15560: HGI48_RS15560 - outer membrane protein assembly factor BamB, at 3,498,840 to 3,500,021
_RS15560
HGI48_RS15565: HGI48_RS15565 - YfgM family protein, at 3,500,032 to 3,500,652
_RS15565
HGI48_RS15570: HGI48_RS15570 - histidine--tRNA ligase, at 3,500,666 to 3,501,940
_RS15570
Position (kb)
3498
3499
3500
3501 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3498.758 kb on - strand at 3499.022 kb on - strand, within HGI48_RS15560 at 3499.022 kb on - strand, within HGI48_RS15560 at 3499.078 kb on - strand, within HGI48_RS15560 at 3499.134 kb on - strand, within HGI48_RS15560 at 3499.230 kb on - strand at 3499.230 kb on - strand, within HGI48_RS15560 at 3499.244 kb on - strand at 3499.244 kb on - strand, within HGI48_RS15560 at 3499.844 kb on - strand, within HGI48_RS15560 at 3500.111 kb on + strand, within HGI48_RS15565 at 3500.112 kb on - strand, within HGI48_RS15565 at 3500.112 kb on - strand, within HGI48_RS15565 at 3500.252 kb on - strand, within HGI48_RS15565 at 3500.509 kb on - strand, within HGI48_RS15565 at 3500.643 kb on - strand at 3500.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 3,498,758 - -0.4 3,499,022 - HGI48_RS15560 0.15 +3.3 3,499,022 - HGI48_RS15560 0.15 +1.1 3,499,078 - HGI48_RS15560 0.20 +1.1 3,499,134 - HGI48_RS15560 0.25 +2.0 3,499,230 - -0.2 3,499,230 - HGI48_RS15560 0.33 +1.9 3,499,244 - -1.9 3,499,244 - HGI48_RS15560 0.34 -0.2 3,499,844 - HGI48_RS15560 0.85 +2.3 3,500,111 + HGI48_RS15565 0.13 -0.1 3,500,112 - HGI48_RS15565 0.13 +0.4 3,500,112 - HGI48_RS15565 0.13 -0.1 3,500,252 - HGI48_RS15565 0.35 +1.9 3,500,509 - HGI48_RS15565 0.77 +0.8 3,500,643 - -0.1 3,500,643 - -0.4
Or see this region's nucleotide sequence