Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS14355 and HGI48_RS14360 are separated by 111 nucleotides HGI48_RS14360 and HGI48_RS14365 are separated by 90 nucleotides HGI48_RS14365 and HGI48_RS14370 are separated by 184 nucleotides
HGI48_RS14355: HGI48_RS14355 - phosphate acetyltransferase, at 3,224,952 to 3,227,093
_RS14355
HGI48_RS14360: HGI48_RS14360 - NUDIX hydrolase YfcD, at 3,227,205 to 3,227,819
_RS14360
HGI48_RS14365: HGI48_RS14365 - phosphodiesterase, at 3,227,910 to 3,228,461
_RS14365
HGI48_RS14370: HGI48_RS14370 - glutathione S-transferase family protein, at 3,228,646 to 3,228,987
_RS14370
Position (kb)
3227
3228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3226.321 kb on + strand, within HGI48_RS14355 at 3226.350 kb on + strand, within HGI48_RS14355 at 3226.354 kb on + strand, within HGI48_RS14355 at 3226.354 kb on + strand, within HGI48_RS14355 at 3226.603 kb on - strand, within HGI48_RS14355 at 3226.720 kb on - strand, within HGI48_RS14355 at 3226.730 kb on + strand, within HGI48_RS14355 at 3226.938 kb on + strand at 3226.939 kb on - strand at 3227.103 kb on + strand at 3227.132 kb on + strand at 3227.132 kb on + strand at 3227.132 kb on + strand at 3227.132 kb on + strand at 3227.133 kb on - strand at 3227.133 kb on - strand at 3227.133 kb on - strand at 3227.133 kb on - strand at 3227.133 kb on - strand at 3227.133 kb on - strand at 3227.296 kb on + strand, within HGI48_RS14360 at 3227.296 kb on + strand, within HGI48_RS14360 at 3227.296 kb on + strand, within HGI48_RS14360 at 3227.296 kb on + strand, within HGI48_RS14360 at 3227.297 kb on - strand at 3227.297 kb on - strand, within HGI48_RS14360 at 3227.297 kb on - strand, within HGI48_RS14360 at 3227.297 kb on - strand, within HGI48_RS14360 at 3227.297 kb on - strand, within HGI48_RS14360 at 3227.342 kb on + strand, within HGI48_RS14360 at 3227.448 kb on + strand, within HGI48_RS14360 at 3227.448 kb on + strand, within HGI48_RS14360 at 3227.449 kb on - strand at 3227.497 kb on + strand, within HGI48_RS14360 at 3227.497 kb on + strand, within HGI48_RS14360 at 3227.685 kb on + strand at 3227.685 kb on + strand, within HGI48_RS14360 at 3227.685 kb on + strand, within HGI48_RS14360 at 3227.686 kb on - strand, within HGI48_RS14360 at 3227.686 kb on - strand, within HGI48_RS14360 at 3227.686 kb on - strand, within HGI48_RS14360 at 3227.686 kb on - strand, within HGI48_RS14360 at 3227.695 kb on + strand, within HGI48_RS14360 at 3227.695 kb on + strand, within HGI48_RS14360 at 3227.695 kb on + strand, within HGI48_RS14360 at 3227.695 kb on + strand, within HGI48_RS14360 at 3227.696 kb on - strand, within HGI48_RS14360 at 3227.696 kb on - strand, within HGI48_RS14360 at 3227.696 kb on - strand, within HGI48_RS14360 at 3227.696 kb on - strand, within HGI48_RS14360 at 3227.699 kb on + strand, within HGI48_RS14360 at 3227.700 kb on - strand, within HGI48_RS14360 at 3227.880 kb on + strand at 3227.880 kb on + strand at 3227.880 kb on + strand at 3227.880 kb on + strand at 3227.881 kb on - strand at 3227.881 kb on - strand at 3227.900 kb on - strand at 3227.911 kb on + strand at 3227.912 kb on - strand at 3227.912 kb on - strand at 3227.912 kb on - strand at 3227.936 kb on + strand at 3228.046 kb on + strand, within HGI48_RS14365 at 3228.046 kb on + strand, within HGI48_RS14365 at 3228.046 kb on + strand, within HGI48_RS14365 at 3228.050 kb on + strand, within HGI48_RS14365 at 3228.050 kb on + strand, within HGI48_RS14365 at 3228.051 kb on - strand at 3228.084 kb on - strand, within HGI48_RS14365 at 3228.139 kb on + strand, within HGI48_RS14365 at 3228.139 kb on + strand, within HGI48_RS14365 at 3228.139 kb on + strand, within HGI48_RS14365 at 3228.139 kb on + strand at 3228.139 kb on + strand, within HGI48_RS14365 at 3228.139 kb on + strand, within HGI48_RS14365 at 3228.140 kb on - strand, within HGI48_RS14365 at 3228.140 kb on - strand at 3228.249 kb on - strand at 3228.277 kb on + strand, within HGI48_RS14365 at 3228.485 kb on + strand at 3228.485 kb on + strand at 3228.486 kb on - strand at 3228.486 kb on - strand at 3228.499 kb on - strand at 3228.500 kb on + strand at 3228.500 kb on + strand at 3228.500 kb on + strand at 3228.500 kb on + strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.501 kb on - strand at 3228.504 kb on - strand at 3228.515 kb on - strand at 3228.515 kb on - strand at 3228.601 kb on - strand at 3228.601 kb on - strand at 3228.617 kb on + strand at 3228.617 kb on + strand at 3228.618 kb on - strand at 3228.789 kb on - strand, within HGI48_RS14370 at 3228.789 kb on - strand, within HGI48_RS14370 at 3228.792 kb on + strand, within HGI48_RS14370 at 3228.792 kb on + strand, within HGI48_RS14370
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 3,226,321 + HGI48_RS14355 0.64 +0.7 3,226,350 + HGI48_RS14355 0.65 -2.0 3,226,354 + HGI48_RS14355 0.65 +0.9 3,226,354 + HGI48_RS14355 0.65 +0.1 3,226,603 - HGI48_RS14355 0.77 -2.8 3,226,720 - HGI48_RS14355 0.83 -1.0 3,226,730 + HGI48_RS14355 0.83 +0.7 3,226,938 + -0.0 3,226,939 - -1.5 3,227,103 + -1.8 3,227,132 + -0.0 3,227,132 + -0.2 3,227,132 + -0.0 3,227,132 + -0.4 3,227,133 - -1.6 3,227,133 - +0.3 3,227,133 - +1.0 3,227,133 - +1.5 3,227,133 - -0.0 3,227,133 - +0.7 3,227,296 + HGI48_RS14360 0.15 -0.5 3,227,296 + HGI48_RS14360 0.15 +0.1 3,227,296 + HGI48_RS14360 0.15 -0.1 3,227,296 + HGI48_RS14360 0.15 -0.4 3,227,297 - -1.6 3,227,297 - HGI48_RS14360 0.15 +0.5 3,227,297 - HGI48_RS14360 0.15 -0.2 3,227,297 - HGI48_RS14360 0.15 +0.2 3,227,297 - HGI48_RS14360 0.15 +1.6 3,227,342 + HGI48_RS14360 0.22 +0.1 3,227,448 + HGI48_RS14360 0.40 -1.9 3,227,448 + HGI48_RS14360 0.40 +0.3 3,227,449 - -0.9 3,227,497 + HGI48_RS14360 0.47 +0.3 3,227,497 + HGI48_RS14360 0.47 -1.0 3,227,685 + -0.8 3,227,685 + HGI48_RS14360 0.78 +1.1 3,227,685 + HGI48_RS14360 0.78 -0.7 3,227,686 - HGI48_RS14360 0.78 -1.7 3,227,686 - HGI48_RS14360 0.78 +1.0 3,227,686 - HGI48_RS14360 0.78 -0.6 3,227,686 - HGI48_RS14360 0.78 +0.7 3,227,695 + HGI48_RS14360 0.80 +0.3 3,227,695 + HGI48_RS14360 0.80 -1.1 3,227,695 + HGI48_RS14360 0.80 -2.3 3,227,695 + HGI48_RS14360 0.80 -0.6 3,227,696 - HGI48_RS14360 0.80 -0.3 3,227,696 - HGI48_RS14360 0.80 +1.0 3,227,696 - HGI48_RS14360 0.80 -2.3 3,227,696 - HGI48_RS14360 0.80 +0.8 3,227,699 + HGI48_RS14360 0.80 +0.3 3,227,700 - HGI48_RS14360 0.80 -0.6 3,227,880 + -0.1 3,227,880 + +0.7 3,227,880 + +2.0 3,227,880 + +0.4 3,227,881 - -1.6 3,227,881 - -0.3 3,227,900 - -0.0 3,227,911 + -1.0 3,227,912 - +0.6 3,227,912 - +0.4 3,227,912 - +0.4 3,227,936 + -1.8 3,228,046 + HGI48_RS14365 0.25 +1.0 3,228,046 + HGI48_RS14365 0.25 -0.5 3,228,046 + HGI48_RS14365 0.25 -1.8 3,228,050 + HGI48_RS14365 0.25 +0.8 3,228,050 + HGI48_RS14365 0.25 -1.2 3,228,051 - -0.0 3,228,084 - HGI48_RS14365 0.32 -0.0 3,228,139 + HGI48_RS14365 0.41 -1.1 3,228,139 + HGI48_RS14365 0.41 -1.0 3,228,139 + HGI48_RS14365 0.41 +0.1 3,228,139 + -1.8 3,228,139 + HGI48_RS14365 0.41 -0.6 3,228,139 + HGI48_RS14365 0.41 -0.7 3,228,140 - HGI48_RS14365 0.42 +0.7 3,228,140 - -1.8 3,228,249 - +0.3 3,228,277 + HGI48_RS14365 0.66 -0.2 3,228,485 + -1.6 3,228,485 + +1.0 3,228,486 - +0.6 3,228,486 - -0.0 3,228,499 - -0.2 3,228,500 + -1.8 3,228,500 + -0.6 3,228,500 + -2.3 3,228,500 + +0.4 3,228,501 - -0.6 3,228,501 - +2.0 3,228,501 - -1.0 3,228,501 - -2.3 3,228,501 - -2.1 3,228,501 - -0.6 3,228,501 - +0.2 3,228,501 - -1.0 3,228,501 - -0.6 3,228,504 - -1.8 3,228,515 - +0.2 3,228,515 - +0.5 3,228,601 - -2.3 3,228,601 - +0.7 3,228,617 + -0.0 3,228,617 + +0.3 3,228,618 - +0.3 3,228,789 - HGI48_RS14370 0.42 -0.8 3,228,789 - HGI48_RS14370 0.42 -1.8 3,228,792 + HGI48_RS14370 0.43 -2.0 3,228,792 + HGI48_RS14370 0.43 -1.8
Or see this region's nucleotide sequence