Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS07875 and HGI48_RS07880 overlap by 8 nucleotides HGI48_RS07880 and HGI48_RS07885 overlap by 4 nucleotides HGI48_RS07885 and HGI48_RS07890 are separated by 472 nucleotides
HGI48_RS07875: HGI48_RS07875 - metal ABC transporter permease, at 1,684,508 to 1,685,362
_RS07875
HGI48_RS07880: HGI48_RS07880 - manganese/iron ABC transporter ATP-binding protein, at 1,685,355 to 1,686,251
_RS07880
HGI48_RS07885: HGI48_RS07885 - metal ABC transporter substrate-binding protein, at 1,686,248 to 1,687,165
_RS07885
HGI48_RS07890: HGI48_RS07890 - iron-containing alcohol dehydrogenase, at 1,687,638 to 1,688,786
_RS07890
Position (kb)
1686
1687
1688 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1685.262 kb on + strand, within HGI48_RS07875 at 1685.304 kb on - strand at 1685.325 kb on + strand at 1685.325 kb on + strand at 1685.325 kb on + strand at 1685.325 kb on + strand at 1685.325 kb on + strand at 1685.325 kb on + strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.326 kb on - strand at 1685.332 kb on - strand at 1685.699 kb on + strand, within HGI48_RS07880 at 1685.718 kb on + strand, within HGI48_RS07880 at 1685.718 kb on + strand, within HGI48_RS07880 at 1685.718 kb on + strand, within HGI48_RS07880 at 1685.719 kb on - strand, within HGI48_RS07880 at 1685.824 kb on - strand at 1685.824 kb on - strand, within HGI48_RS07880 at 1685.977 kb on - strand at 1685.977 kb on - strand at 1685.998 kb on + strand, within HGI48_RS07880 at 1685.998 kb on + strand, within HGI48_RS07880 at 1685.998 kb on + strand, within HGI48_RS07880 at 1685.999 kb on - strand, within HGI48_RS07880 at 1686.063 kb on - strand, within HGI48_RS07880 at 1686.196 kb on + strand at 1686.196 kb on + strand at 1686.197 kb on - strand at 1686.197 kb on - strand at 1686.256 kb on + strand at 1686.257 kb on - strand at 1686.303 kb on + strand at 1686.304 kb on - strand at 1686.345 kb on + strand, within HGI48_RS07885 at 1686.346 kb on - strand, within HGI48_RS07885 at 1686.346 kb on - strand, within HGI48_RS07885 at 1686.346 kb on - strand, within HGI48_RS07885 at 1686.346 kb on - strand, within HGI48_RS07885 at 1686.516 kb on + strand, within HGI48_RS07885 at 1686.516 kb on + strand, within HGI48_RS07885 at 1686.516 kb on + strand at 1686.516 kb on + strand, within HGI48_RS07885 at 1686.517 kb on - strand, within HGI48_RS07885 at 1686.517 kb on - strand at 1686.517 kb on - strand at 1686.550 kb on - strand, within HGI48_RS07885 at 1686.598 kb on + strand, within HGI48_RS07885 at 1686.598 kb on + strand at 1686.598 kb on + strand, within HGI48_RS07885 at 1686.598 kb on + strand, within HGI48_RS07885 at 1686.672 kb on + strand, within HGI48_RS07885 at 1686.672 kb on + strand, within HGI48_RS07885 at 1686.672 kb on + strand, within HGI48_RS07885 at 1686.672 kb on + strand at 1686.673 kb on - strand, within HGI48_RS07885 at 1686.729 kb on + strand, within HGI48_RS07885 at 1686.730 kb on - strand, within HGI48_RS07885 at 1686.783 kb on + strand, within HGI48_RS07885 at 1686.952 kb on - strand, within HGI48_RS07885 at 1687.033 kb on + strand, within HGI48_RS07885 at 1687.039 kb on + strand, within HGI48_RS07885 at 1687.040 kb on - strand, within HGI48_RS07885 at 1687.177 kb on - strand at 1687.198 kb on + strand at 1687.199 kb on - strand at 1687.199 kb on - strand at 1687.228 kb on + strand at 1687.250 kb on + strand at 1687.251 kb on - strand at 1687.251 kb on - strand at 1687.251 kb on - strand at 1687.251 kb on - strand at 1687.251 kb on - strand at 1687.263 kb on + strand at 1687.263 kb on + strand at 1687.264 kb on - strand at 1687.264 kb on - strand at 1687.280 kb on + strand at 1687.293 kb on + strand at 1687.319 kb on - strand at 1687.338 kb on + strand at 1687.366 kb on + strand at 1687.366 kb on + strand at 1687.366 kb on + strand at 1687.366 kb on + strand at 1687.366 kb on + strand at 1687.366 kb on + strand at 1687.367 kb on - strand at 1687.367 kb on - strand at 1687.367 kb on - strand at 1687.367 kb on - strand at 1687.372 kb on + strand at 1687.462 kb on + strand at 1687.493 kb on + strand at 1687.493 kb on + strand at 1687.494 kb on - strand at 1687.494 kb on - strand at 1687.494 kb on - strand at 1687.540 kb on + strand at 1687.544 kb on + strand at 1687.544 kb on + strand at 1687.545 kb on - strand at 1687.640 kb on - strand at 1687.640 kb on - strand at 1687.640 kb on - strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.681 kb on + strand at 1687.682 kb on - strand at 1687.682 kb on - strand at 1687.894 kb on + strand at 1687.894 kb on + strand at 1687.894 kb on + strand, within HGI48_RS07890 at 1687.894 kb on + strand, within HGI48_RS07890 at 1687.895 kb on - strand, within HGI48_RS07890 at 1687.895 kb on - strand, within HGI48_RS07890 at 1688.072 kb on - strand at 1688.160 kb on + strand, within HGI48_RS07890 at 1688.161 kb on - strand, within HGI48_RS07890 at 1688.161 kb on - strand, within HGI48_RS07890
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 1,685,262 + HGI48_RS07875 0.88 -0.9 1,685,304 - -1.5 1,685,325 + -0.4 1,685,325 + -2.5 1,685,325 + -1.4 1,685,325 + -2.1 1,685,325 + +0.9 1,685,325 + -2.4 1,685,326 - -1.1 1,685,326 - -0.1 1,685,326 - -1.3 1,685,326 - -1.9 1,685,326 - -1.4 1,685,326 - +0.1 1,685,326 - -1.2 1,685,326 - -0.8 1,685,326 - +0.4 1,685,326 - -2.1 1,685,332 - -0.9 1,685,699 + HGI48_RS07880 0.38 -1.4 1,685,718 + HGI48_RS07880 0.40 -2.2 1,685,718 + HGI48_RS07880 0.40 -1.9 1,685,718 + HGI48_RS07880 0.40 +0.2 1,685,719 - HGI48_RS07880 0.41 -1.8 1,685,824 - -2.4 1,685,824 - HGI48_RS07880 0.52 -0.4 1,685,977 - -1.9 1,685,977 - -1.6 1,685,998 + HGI48_RS07880 0.72 -2.5 1,685,998 + HGI48_RS07880 0.72 -1.1 1,685,998 + HGI48_RS07880 0.72 -1.9 1,685,999 - HGI48_RS07880 0.72 -1.8 1,686,063 - HGI48_RS07880 0.79 -2.7 1,686,196 + -0.9 1,686,196 + -1.9 1,686,197 - -2.5 1,686,197 - -3.9 1,686,256 + -1.1 1,686,257 - -0.4 1,686,303 + -1.4 1,686,304 - -1.3 1,686,345 + HGI48_RS07885 0.11 +0.3 1,686,346 - HGI48_RS07885 0.11 -1.1 1,686,346 - HGI48_RS07885 0.11 -0.5 1,686,346 - HGI48_RS07885 0.11 -2.6 1,686,346 - HGI48_RS07885 0.11 +0.5 1,686,516 + HGI48_RS07885 0.29 -1.6 1,686,516 + HGI48_RS07885 0.29 -2.1 1,686,516 + -1.9 1,686,516 + HGI48_RS07885 0.29 +0.9 1,686,517 - HGI48_RS07885 0.29 -1.0 1,686,517 - -1.6 1,686,517 - -1.6 1,686,550 - HGI48_RS07885 0.33 -1.5 1,686,598 + HGI48_RS07885 0.38 +0.3 1,686,598 + -1.6 1,686,598 + HGI48_RS07885 0.38 -1.2 1,686,598 + HGI48_RS07885 0.38 -1.2 1,686,672 + HGI48_RS07885 0.46 -1.1 1,686,672 + HGI48_RS07885 0.46 -1.1 1,686,672 + HGI48_RS07885 0.46 -2.4 1,686,672 + -2.2 1,686,673 - HGI48_RS07885 0.46 -2.6 1,686,729 + HGI48_RS07885 0.52 -1.4 1,686,730 - HGI48_RS07885 0.53 +0.9 1,686,783 + HGI48_RS07885 0.58 -1.4 1,686,952 - HGI48_RS07885 0.77 -3.0 1,687,033 + HGI48_RS07885 0.86 -2.4 1,687,039 + HGI48_RS07885 0.86 -2.1 1,687,040 - HGI48_RS07885 0.86 -1.2 1,687,177 - +0.2 1,687,198 + -1.6 1,687,199 - -1.7 1,687,199 - -1.6 1,687,228 + -1.6 1,687,250 + -0.5 1,687,251 - -0.3 1,687,251 - -0.9 1,687,251 - -0.6 1,687,251 - -0.9 1,687,251 - -0.1 1,687,263 + -1.6 1,687,263 + -0.8 1,687,264 - -0.9 1,687,264 - -0.9 1,687,280 + -1.6 1,687,293 + -0.5 1,687,319 - -1.1 1,687,338 + -0.1 1,687,366 + -0.2 1,687,366 + +0.5 1,687,366 + +0.2 1,687,366 + +0.5 1,687,366 + -0.2 1,687,366 + -0.1 1,687,367 - -0.4 1,687,367 - -0.4 1,687,367 - -1.6 1,687,367 - -0.6 1,687,372 + -1.6 1,687,462 + +0.7 1,687,493 + -0.5 1,687,493 + -1.5 1,687,494 - -1.6 1,687,494 - -1.9 1,687,494 - -1.4 1,687,540 + +0.7 1,687,544 + -1.9 1,687,544 + -0.8 1,687,545 - +0.4 1,687,640 - -0.9 1,687,640 - -1.6 1,687,640 - -0.2 1,687,681 + -0.1 1,687,681 + -1.4 1,687,681 + +0.4 1,687,681 + +0.3 1,687,681 + +0.4 1,687,681 + +0.1 1,687,681 + -1.6 1,687,681 + -0.5 1,687,681 + +0.1 1,687,682 - -0.9 1,687,682 - -0.2 1,687,894 + -0.6 1,687,894 + -1.6 1,687,894 + HGI48_RS07890 0.22 +1.6 1,687,894 + HGI48_RS07890 0.22 -0.3 1,687,895 - HGI48_RS07890 0.22 -0.2 1,687,895 - HGI48_RS07890 0.22 -2.2 1,688,072 - -1.4 1,688,160 + HGI48_RS07890 0.45 +0.4 1,688,161 - HGI48_RS07890 0.46 +0.8 1,688,161 - HGI48_RS07890 0.46 -1.1
Or see this region's nucleotide sequence