Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS15625 and HGI48_RS15630 are separated by 77 nucleotides HGI48_RS15630 and HGI48_RS15635 are separated by 97 nucleotides HGI48_RS15635 and HGI48_RS15640 are separated by 25 nucleotides HGI48_RS15640 and HGI48_RS15645 are separated by 24 nucleotides
HGI48_RS15625: HGI48_RS15625 - Fe-S protein assembly chaperone HscA, at 3,511,533 to 3,513,383
_RS15625
HGI48_RS15630: HGI48_RS15630 - co-chaperone HscB, at 3,513,461 to 3,513,979
_RS15630
HGI48_RS15635: HGI48_RS15635 - iron-sulfur cluster assembly protein IscA, at 3,514,077 to 3,514,400
_RS15635
HGI48_RS15640: HGI48_RS15640 - Fe-S cluster assembly scaffold IscU, at 3,514,426 to 3,514,809
_RS15640
HGI48_RS15645: HGI48_RS15645 - cysteine desulfurase, at 3,514,834 to 3,516,048
_RS15645
Position (kb)
3513
3514 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3513.529 kb on + strand, within HGI48_RS15630 at 3513.769 kb on + strand, within HGI48_RS15630 at 3513.770 kb on - strand, within HGI48_RS15630 at 3513.772 kb on - strand, within HGI48_RS15630 at 3513.942 kb on + strand at 3514.024 kb on + strand at 3514.086 kb on - strand at 3514.190 kb on - strand, within HGI48_RS15635 at 3514.401 kb on - strand at 3514.813 kb on - strand at 3514.836 kb on - strand at 3514.836 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 3,513,529 + HGI48_RS15630 0.13 -3.5 3,513,769 + HGI48_RS15630 0.59 -0.9 3,513,770 - HGI48_RS15630 0.60 -1.6 3,513,772 - HGI48_RS15630 0.60 -1.8 3,513,942 + +0.1 3,514,024 + -1.9 3,514,086 - -1.0 3,514,190 - HGI48_RS15635 0.35 -1.4 3,514,401 - -2.4 3,514,813 - -2.0 3,514,836 - -0.9 3,514,836 - +0.2
Or see this region's nucleotide sequence