Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS05565 and HGI48_RS05570 are separated by 120 nucleotides HGI48_RS05570 and HGI48_RS05575 are separated by 22 nucleotides HGI48_RS05575 and HGI48_RS05580 are separated by 60 nucleotides
HGI48_RS05565: HGI48_RS05565 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 1,173,527 to 1,174,639
_RS05565
HGI48_RS05570: HGI48_RS05570 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,174,760 to 1,175,233
_RS05570
HGI48_RS05575: HGI48_RS05575 - transcription antitermination factor NusB, at 1,175,256 to 1,175,675
_RS05575
HGI48_RS05580: HGI48_RS05580 - thiamine-phosphate kinase, at 1,175,736 to 1,176,710
_RS05580
Position (kb)
1175
1176 Strain fitness (log2 ratio)
-2
-1
0
1 at 1174.709 kb on + strand at 1174.709 kb on + strand at 1174.709 kb on + strand at 1174.709 kb on + strand at 1174.710 kb on - strand at 1174.710 kb on - strand at 1174.710 kb on - strand at 1174.714 kb on + strand at 1174.714 kb on + strand at 1174.714 kb on + strand at 1174.714 kb on + strand at 1174.714 kb on + strand at 1175.716 kb on + strand at 1175.719 kb on + strand at 1175.719 kb on + strand at 1175.719 kb on + strand at 1175.735 kb on + strand at 1175.735 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 1,174,709 + +1.0 1,174,709 + -0.2 1,174,709 + +0.6 1,174,709 + +1.1 1,174,710 - -0.6 1,174,710 - -1.3 1,174,710 - +0.3 1,174,714 + +1.4 1,174,714 + -1.2 1,174,714 + -1.2 1,174,714 + -1.2 1,174,714 + -0.3 1,175,716 + +0.5 1,175,719 + +0.6 1,175,719 + -1.0 1,175,719 + -0.6 1,175,735 + -2.6 1,175,735 + -1.0
Or see this region's nucleotide sequence