Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS03135 and HGI48_RS03140 are separated by 70 nucleotides HGI48_RS03140 and HGI48_RS03145 are separated by 188 nucleotides HGI48_RS03145 and HGI48_RS03150 are separated by 226 nucleotides HGI48_RS03150 and HGI48_RS03155 are separated by 1 nucleotides
HGI48_RS03135: HGI48_RS03135 - malate dehydrogenase, at 665,503 to 666,444
_RS03135
HGI48_RS03140: HGI48_RS03140 - helix-turn-helix domain-containing protein, at 666,515 to 666,796
_RS03140
HGI48_RS03145: HGI48_RS03145 - putative DNA-binding transcriptional regulator, at 666,985 to 667,371
_RS03145
HGI48_RS03150: HGI48_RS03150 - putative DNA-binding transcriptional regulator, at 667,598 to 667,987
_RS03150
HGI48_RS03155: HGI48_RS03155 - octaprenyl diphosphate synthase, at 667,989 to 668,960
_RS03155
Position (kb) 666
667
668 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 666.111 kb on + strand, within HGI48_RS03135 at 666.180 kb on + strand, within HGI48_RS03135 at 666.442 kb on + strand at 666.442 kb on + strand at 666.443 kb on - strand at 666.513 kb on + strand at 666.513 kb on + strand at 666.513 kb on + strand at 666.513 kb on + strand at 666.513 kb on + strand at 666.513 kb on + strand at 666.514 kb on - strand at 666.517 kb on - strand at 666.567 kb on - strand, within HGI48_RS03140 at 666.567 kb on - strand, within HGI48_RS03140 at 666.588 kb on + strand, within HGI48_RS03140 at 666.588 kb on + strand, within HGI48_RS03140 at 666.588 kb on + strand, within HGI48_RS03140 at 666.588 kb on + strand at 666.588 kb on + strand, within HGI48_RS03140 at 666.589 kb on - strand, within HGI48_RS03140 at 666.589 kb on - strand, within HGI48_RS03140 at 666.589 kb on - strand, within HGI48_RS03140 at 666.591 kb on + strand, within HGI48_RS03140 at 666.592 kb on - strand, within HGI48_RS03140 at 666.606 kb on - strand, within HGI48_RS03140 at 666.639 kb on - strand at 666.672 kb on - strand, within HGI48_RS03140 at 666.716 kb on + strand, within HGI48_RS03140 at 666.716 kb on + strand, within HGI48_RS03140 at 666.717 kb on - strand, within HGI48_RS03140 at 666.717 kb on - strand, within HGI48_RS03140 at 666.757 kb on + strand, within HGI48_RS03140 at 666.757 kb on + strand, within HGI48_RS03140 at 666.758 kb on - strand, within HGI48_RS03140 at 666.758 kb on - strand, within HGI48_RS03140 at 666.797 kb on - strand at 666.814 kb on + strand at 666.814 kb on + strand at 666.814 kb on + strand at 666.815 kb on - strand at 666.815 kb on - strand at 666.817 kb on + strand at 666.830 kb on + strand at 666.830 kb on + strand at 666.830 kb on + strand at 666.831 kb on - strand at 666.833 kb on + strand at 666.833 kb on + strand at 666.839 kb on + strand at 666.839 kb on + strand at 666.839 kb on + strand at 666.839 kb on + strand at 666.839 kb on + strand at 666.841 kb on + strand at 666.841 kb on + strand at 666.841 kb on + strand at 666.841 kb on + strand at 666.841 kb on + strand at 666.842 kb on - strand at 666.842 kb on - strand at 666.842 kb on - strand at 666.842 kb on - strand at 666.842 kb on - strand at 666.896 kb on - strand at 666.951 kb on - strand at 666.951 kb on - strand at 666.962 kb on - strand at 667.060 kb on - strand, within HGI48_RS03145 at 667.126 kb on + strand, within HGI48_RS03145 at 667.127 kb on - strand, within HGI48_RS03145 at 667.132 kb on - strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.212 kb on + strand, within HGI48_RS03145 at 667.213 kb on - strand, within HGI48_RS03145 at 667.213 kb on - strand at 667.213 kb on - strand, within HGI48_RS03145 at 667.213 kb on - strand, within HGI48_RS03145 at 667.213 kb on - strand, within HGI48_RS03145 at 667.238 kb on - strand, within HGI48_RS03145 at 667.247 kb on + strand, within HGI48_RS03145 at 667.247 kb on + strand, within HGI48_RS03145 at 667.247 kb on + strand at 667.247 kb on + strand, within HGI48_RS03145 at 667.247 kb on + strand, within HGI48_RS03145 at 667.247 kb on + strand at 667.247 kb on + strand, within HGI48_RS03145 at 667.248 kb on - strand, within HGI48_RS03145 at 667.248 kb on - strand, within HGI48_RS03145 at 667.248 kb on - strand, within HGI48_RS03145 at 667.248 kb on - strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.279 kb on + strand, within HGI48_RS03145 at 667.280 kb on - strand, within HGI48_RS03145 at 667.280 kb on - strand at 667.280 kb on - strand, within HGI48_RS03145 at 667.280 kb on - strand, within HGI48_RS03145 at 667.280 kb on - strand, within HGI48_RS03145 at 667.280 kb on - strand, within HGI48_RS03145 at 667.281 kb on + strand, within HGI48_RS03145 at 667.281 kb on + strand, within HGI48_RS03145 at 667.281 kb on + strand, within HGI48_RS03145 at 667.281 kb on + strand, within HGI48_RS03145 at 667.282 kb on - strand, within HGI48_RS03145 at 667.282 kb on - strand, within HGI48_RS03145 at 667.282 kb on - strand, within HGI48_RS03145 at 667.282 kb on - strand, within HGI48_RS03145 at 667.369 kb on + strand at 667.370 kb on - strand at 667.370 kb on - strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.469 kb on + strand at 667.470 kb on - strand at 667.470 kb on - strand at 667.470 kb on - strand at 667.470 kb on - strand at 667.470 kb on - strand at 667.475 kb on + strand at 667.475 kb on + strand at 667.476 kb on - strand at 667.561 kb on + strand at 667.568 kb on - strand at 667.632 kb on + strand at 667.633 kb on - strand at 667.672 kb on + strand, within HGI48_RS03150 at 667.673 kb on - strand, within HGI48_RS03150 at 667.739 kb on + strand, within HGI48_RS03150 at 667.744 kb on + strand, within HGI48_RS03150 at 667.767 kb on + strand, within HGI48_RS03150 at 667.767 kb on + strand, within HGI48_RS03150 at 667.802 kb on + strand, within HGI48_RS03150 at 667.851 kb on - strand, within HGI48_RS03150 at 667.894 kb on + strand, within HGI48_RS03150 at 667.894 kb on + strand, within HGI48_RS03150 at 667.894 kb on + strand, within HGI48_RS03150 at 667.895 kb on - strand, within HGI48_RS03150 at 667.895 kb on - strand, within HGI48_RS03150 at 667.918 kb on + strand, within HGI48_RS03150 at 667.918 kb on + strand, within HGI48_RS03150 at 667.919 kb on - strand, within HGI48_RS03150 at 667.919 kb on - strand, within HGI48_RS03150 at 667.919 kb on - strand, within HGI48_RS03150 at 667.953 kb on - strand at 667.953 kb on - strand at 667.955 kb on - strand at 667.955 kb on - strand at 667.967 kb on + strand at 667.968 kb on - strand at 667.968 kb on - strand at 667.968 kb on - strand at 667.968 kb on - strand at 667.972 kb on + strand at 667.973 kb on - strand at 667.990 kb on + strand at 667.990 kb on + strand at 667.990 kb on + strand at 667.991 kb on - strand at 667.991 kb on - strand at 667.991 kb on - strand at 667.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 666,111 + HGI48_RS03135 0.65 -1.7 666,180 + HGI48_RS03135 0.72 -1.9 666,442 + -1.5 666,442 + -0.9 666,443 - -1.2 666,513 + -2.1 666,513 + -3.0 666,513 + +1.0 666,513 + -1.4 666,513 + +0.1 666,513 + -0.5 666,514 - -2.2 666,517 - -2.0 666,567 - HGI48_RS03140 0.18 +1.2 666,567 - HGI48_RS03140 0.18 -0.5 666,588 + HGI48_RS03140 0.26 -2.4 666,588 + HGI48_RS03140 0.26 -2.2 666,588 + HGI48_RS03140 0.26 -0.2 666,588 + -1.2 666,588 + HGI48_RS03140 0.26 -0.1 666,589 - HGI48_RS03140 0.26 +0.7 666,589 - HGI48_RS03140 0.26 -0.3 666,589 - HGI48_RS03140 0.26 +0.0 666,591 + HGI48_RS03140 0.27 -0.3 666,592 - HGI48_RS03140 0.27 -1.2 666,606 - HGI48_RS03140 0.32 +0.1 666,639 - -0.5 666,672 - HGI48_RS03140 0.56 -1.5 666,716 + HGI48_RS03140 0.71 -0.4 666,716 + HGI48_RS03140 0.71 -0.9 666,717 - HGI48_RS03140 0.72 +0.3 666,717 - HGI48_RS03140 0.72 -2.1 666,757 + HGI48_RS03140 0.86 +0.9 666,757 + HGI48_RS03140 0.86 +0.0 666,758 - HGI48_RS03140 0.86 -0.5 666,758 - HGI48_RS03140 0.86 -2.5 666,797 - -0.6 666,814 + -2.4 666,814 + -1.5 666,814 + -2.2 666,815 - -1.4 666,815 - +3.4 666,817 + -0.1 666,830 + -1.1 666,830 + -0.4 666,830 + +2.9 666,831 - -1.7 666,833 + -0.2 666,833 + -0.5 666,839 + -0.2 666,839 + +0.4 666,839 + +0.4 666,839 + +1.6 666,839 + +0.7 666,841 + -0.7 666,841 + +0.5 666,841 + -1.7 666,841 + -1.9 666,841 + -1.2 666,842 - -1.7 666,842 - -0.3 666,842 - -1.3 666,842 - +0.0 666,842 - -0.4 666,896 - -0.9 666,951 - +0.4 666,951 - -0.4 666,962 - -0.1 667,060 - HGI48_RS03145 0.19 -0.5 667,126 + HGI48_RS03145 0.36 -0.3 667,127 - HGI48_RS03145 0.37 -0.4 667,132 - HGI48_RS03145 0.38 +1.7 667,212 + HGI48_RS03145 0.59 -0.9 667,212 + HGI48_RS03145 0.59 +0.1 667,212 + HGI48_RS03145 0.59 -0.7 667,212 + HGI48_RS03145 0.59 -1.1 667,212 + HGI48_RS03145 0.59 -1.5 667,212 + HGI48_RS03145 0.59 -0.7 667,212 + HGI48_RS03145 0.59 +2.3 667,212 + HGI48_RS03145 0.59 +0.3 667,212 + HGI48_RS03145 0.59 -0.2 667,212 + HGI48_RS03145 0.59 -0.2 667,213 - HGI48_RS03145 0.59 -2.4 667,213 - +0.5 667,213 - HGI48_RS03145 0.59 +0.8 667,213 - HGI48_RS03145 0.59 -0.4 667,213 - HGI48_RS03145 0.59 +2.3 667,238 - HGI48_RS03145 0.65 +0.8 667,247 + HGI48_RS03145 0.68 -0.5 667,247 + HGI48_RS03145 0.68 +0.2 667,247 + -1.2 667,247 + HGI48_RS03145 0.68 -0.4 667,247 + HGI48_RS03145 0.68 -1.4 667,247 + +0.1 667,247 + HGI48_RS03145 0.68 -0.9 667,248 - HGI48_RS03145 0.68 +0.0 667,248 - HGI48_RS03145 0.68 -1.1 667,248 - HGI48_RS03145 0.68 -0.5 667,248 - HGI48_RS03145 0.68 +1.0 667,279 + HGI48_RS03145 0.76 -0.1 667,279 + HGI48_RS03145 0.76 +0.1 667,279 + HGI48_RS03145 0.76 -0.9 667,279 + HGI48_RS03145 0.76 -0.2 667,279 + -0.7 667,279 + HGI48_RS03145 0.76 +0.5 667,279 + HGI48_RS03145 0.76 -0.4 667,279 + HGI48_RS03145 0.76 +1.1 667,279 + HGI48_RS03145 0.76 +0.8 667,279 + HGI48_RS03145 0.76 +1.2 667,279 + HGI48_RS03145 0.76 -0.7 667,280 - HGI48_RS03145 0.76 +0.3 667,280 - -1.8 667,280 - HGI48_RS03145 0.76 -0.5 667,280 - HGI48_RS03145 0.76 +0.2 667,280 - HGI48_RS03145 0.76 +0.7 667,280 - HGI48_RS03145 0.76 +1.1 667,281 + HGI48_RS03145 0.76 -0.3 667,281 + HGI48_RS03145 0.76 -0.1 667,281 + HGI48_RS03145 0.76 -0.1 667,281 + HGI48_RS03145 0.76 +0.4 667,282 - HGI48_RS03145 0.77 +0.1 667,282 - HGI48_RS03145 0.77 -0.5 667,282 - HGI48_RS03145 0.77 -1.2 667,282 - HGI48_RS03145 0.77 +1.3 667,369 + -2.1 667,370 - -0.0 667,370 - +1.1 667,469 + +1.1 667,469 + +1.5 667,469 + +0.8 667,469 + +0.9 667,469 + +0.7 667,469 + +1.4 667,469 + +0.2 667,469 + +1.2 667,469 + +0.4 667,470 - -2.4 667,470 - +1.6 667,470 - +0.2 667,470 - +0.7 667,470 - -0.8 667,475 + +2.0 667,475 + -0.4 667,476 - -2.1 667,561 + -1.9 667,568 - +1.0 667,632 + -1.7 667,633 - +1.1 667,672 + HGI48_RS03150 0.19 -0.5 667,673 - HGI48_RS03150 0.19 +0.1 667,739 + HGI48_RS03150 0.36 -1.9 667,744 + HGI48_RS03150 0.37 +0.4 667,767 + HGI48_RS03150 0.43 -0.1 667,767 + HGI48_RS03150 0.43 -0.2 667,802 + HGI48_RS03150 0.52 -0.8 667,851 - HGI48_RS03150 0.65 -1.5 667,894 + HGI48_RS03150 0.76 +0.7 667,894 + HGI48_RS03150 0.76 -0.9 667,894 + HGI48_RS03150 0.76 +1.1 667,895 - HGI48_RS03150 0.76 -0.9 667,895 - HGI48_RS03150 0.76 +0.5 667,918 + HGI48_RS03150 0.82 -0.9 667,918 + HGI48_RS03150 0.82 -1.1 667,919 - HGI48_RS03150 0.82 -1.5 667,919 - HGI48_RS03150 0.82 +1.1 667,919 - HGI48_RS03150 0.82 -0.2 667,953 - +0.7 667,953 - -0.6 667,955 - +0.8 667,955 - -1.1 667,967 + -0.9 667,968 - -0.1 667,968 - -0.1 667,968 - +1.1 667,968 - -0.2 667,972 + +0.9 667,973 - -2.2 667,990 + +0.3 667,990 + -3.4 667,990 + -2.5 667,991 - +0.5 667,991 - -0.1 667,991 - -0.1 667,991 - -1.2
Or see this region's nucleotide sequence