Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HGI48_RS01215 and HGI48_RS01220 are separated by 599 nucleotides HGI48_RS01220 and HGI48_RS01225 are separated by 87 nucleotides HGI48_RS01225 and HGI48_RS01230 are separated by 194 nucleotides
HGI48_RS01215: HGI48_RS01215 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 270,515 to 272,104
_RS01215
HGI48_RS01220: HGI48_RS01220 - 16S ribosomal RNA, at 272,704 to 274,253
_RS01220
HGI48_RS01225: HGI48_RS01225 - tRNA-Glu, at 274,341 to 274,416
_RS01225
HGI48_RS01230: HGI48_RS01230 - 23S ribosomal RNA, at 274,611 to 277,532
_RS01230
Position (kb) 272
273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 271.996 kb on + strand at 271.996 kb on + strand at 272.064 kb on - strand at 272.064 kb on - strand at 272.086 kb on + strand at 272.125 kb on + strand at 272.125 kb on + strand at 272.125 kb on + strand at 272.125 kb on + strand at 272.125 kb on + strand at 272.126 kb on - strand at 272.126 kb on - strand at 272.126 kb on - strand at 272.126 kb on - strand at 272.133 kb on + strand at 272.133 kb on + strand at 272.138 kb on + strand at 272.156 kb on + strand at 272.156 kb on + strand at 272.181 kb on - strand at 272.320 kb on + strand at 272.321 kb on - strand at 272.407 kb on - strand at 272.407 kb on - strand at 272.409 kb on - strand at 272.952 kb on + strand, within HGI48_RS01220 at 272.952 kb on + strand, within HGI48_RS01220 at 272.952 kb on + strand, within HGI48_RS01220 at 272.955 kb on + strand, within HGI48_RS01220 at 274.418 kb on + strand at 274.418 kb on + strand at 274.484 kb on + strand at 274.484 kb on + strand at 275.187 kb on + strand, within HGI48_RS01230 at 275.187 kb on + strand, within HGI48_RS01230 at 275.229 kb on + strand, within HGI48_RS01230 at 275.229 kb on + strand, within HGI48_RS01230
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 271,996 + -3.5 271,996 + -1.9 272,064 - -2.0 272,064 - +0.3 272,086 + +1.2 272,125 + -3.2 272,125 + -1.5 272,125 + -0.0 272,125 + +1.0 272,125 + -0.3 272,126 - -0.0 272,126 - -0.4 272,126 - +1.0 272,126 - -1.7 272,133 + -3.2 272,133 + -0.7 272,138 + -1.7 272,156 + -1.2 272,156 + -1.0 272,181 - -0.3 272,320 + +0.3 272,321 - -0.5 272,407 - -0.5 272,407 - +0.1 272,409 - +1.3 272,952 + HGI48_RS01220 0.16 -0.6 272,952 + HGI48_RS01220 0.16 -1.0 272,952 + HGI48_RS01220 0.16 -2.2 272,955 + HGI48_RS01220 0.16 -1.0 274,418 + +0.8 274,418 + +0.7 274,484 + +0.1 274,484 + +0.9 275,187 + HGI48_RS01230 0.20 +0.1 275,187 + HGI48_RS01230 0.20 -1.8 275,229 + HGI48_RS01230 0.21 +1.3 275,229 + HGI48_RS01230 0.21 -1.3
Or see this region's nucleotide sequence