Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS14305 and DZA65_RS14310 are separated by 75 nucleotides DZA65_RS14310 and DZA65_RS14315 are separated by 384 nucleotides DZA65_RS14315 and DZA65_RS14320 are separated by 161 nucleotides
DZA65_RS14305: DZA65_RS14305 - D-serine/D-alanine/glycine transporter, at 3,123,452 to 3,124,855
_RS14305
DZA65_RS14310: DZA65_RS14310 - LysR family transcriptional regulator, at 3,124,931 to 3,125,197
_RS14310
DZA65_RS14315: DZA65_RS14315 - tRNA-Asn, at 3,125,582 to 3,125,657
_RS14315
DZA65_RS14320: DZA65_RS14320 - integrase arm-type DNA-binding domain-containing protein, at 3,125,819 to 3,127,090
_RS14320
Position (kb) 3125
3126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3124.609 kb on + strand, within DZA65_RS14305 at 3124.609 kb on + strand, within DZA65_RS14305 at 3124.610 kb on - strand, within DZA65_RS14305 at 3124.625 kb on + strand, within DZA65_RS14305 at 3124.625 kb on + strand, within DZA65_RS14305 at 3124.625 kb on + strand, within DZA65_RS14305 at 3124.625 kb on + strand, within DZA65_RS14305 at 3124.626 kb on - strand, within DZA65_RS14305 at 3124.626 kb on - strand, within DZA65_RS14305 at 3124.626 kb on - strand, within DZA65_RS14305 at 3124.673 kb on + strand, within DZA65_RS14305 at 3124.674 kb on - strand, within DZA65_RS14305 at 3124.674 kb on - strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.692 kb on + strand, within DZA65_RS14305 at 3124.695 kb on + strand, within DZA65_RS14305 at 3124.695 kb on + strand, within DZA65_RS14305 at 3124.695 kb on + strand, within DZA65_RS14305 at 3124.695 kb on + strand, within DZA65_RS14305 at 3124.696 kb on - strand, within DZA65_RS14305 at 3124.696 kb on - strand, within DZA65_RS14305 at 3124.696 kb on - strand, within DZA65_RS14305 at 3124.696 kb on - strand, within DZA65_RS14305 at 3124.708 kb on - strand, within DZA65_RS14305 at 3124.738 kb on - strand at 3124.759 kb on + strand at 3124.780 kb on + strand at 3124.823 kb on - strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.858 kb on + strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.859 kb on - strand at 3124.882 kb on - strand at 3124.892 kb on - strand at 3124.892 kb on - strand at 3124.892 kb on - strand at 3124.924 kb on - strand at 3124.924 kb on - strand at 3125.014 kb on + strand, within DZA65_RS14310 at 3125.047 kb on + strand, within DZA65_RS14310 at 3125.048 kb on - strand, within DZA65_RS14310 at 3125.119 kb on + strand, within DZA65_RS14310 at 3125.175 kb on + strand at 3125.176 kb on - strand at 3125.176 kb on - strand at 3125.176 kb on - strand at 3125.201 kb on + strand at 3125.202 kb on - strand at 3125.212 kb on + strand at 3125.216 kb on + strand at 3125.217 kb on - strand at 3125.286 kb on - strand at 3125.301 kb on - strand at 3125.304 kb on + strand at 3125.304 kb on + strand at 3125.332 kb on - strand at 3125.337 kb on + strand at 3125.337 kb on + strand at 3125.337 kb on + strand at 3125.338 kb on - strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.340 kb on + strand at 3125.341 kb on - strand at 3125.341 kb on - strand at 3125.411 kb on + strand at 3125.478 kb on + strand at 3125.478 kb on + strand at 3125.521 kb on + strand at 3125.559 kb on + strand at 3125.576 kb on - strand at 3125.602 kb on - strand, within DZA65_RS14315 at 3125.625 kb on - strand, within DZA65_RS14315 at 3125.692 kb on + strand at 3125.714 kb on - strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.818 kb on + strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.819 kb on - strand at 3125.851 kb on - strand at 3125.864 kb on - strand at 3125.877 kb on + strand at 3125.897 kb on + strand at 3125.897 kb on + strand at 3125.898 kb on - strand at 3125.914 kb on + strand at 3125.914 kb on + strand at 3125.942 kb on + strand at 3125.942 kb on + strand at 3125.943 kb on - strand at 3125.943 kb on - strand at 3125.943 kb on - strand at 3125.943 kb on - strand at 3126.052 kb on + strand, within DZA65_RS14320 at 3126.104 kb on - strand, within DZA65_RS14320 at 3126.104 kb on - strand, within DZA65_RS14320 at 3126.199 kb on + strand, within DZA65_RS14320 at 3126.199 kb on + strand, within DZA65_RS14320 at 3126.199 kb on + strand, within DZA65_RS14320 at 3126.199 kb on + strand, within DZA65_RS14320 at 3126.199 kb on + strand, within DZA65_RS14320 at 3126.200 kb on - strand, within DZA65_RS14320 at 3126.200 kb on - strand, within DZA65_RS14320 at 3126.200 kb on - strand, within DZA65_RS14320 at 3126.200 kb on - strand, within DZA65_RS14320 at 3126.200 kb on - strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.319 kb on + strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.320 kb on - strand, within DZA65_RS14320 at 3126.360 kb on + strand, within DZA65_RS14320 at 3126.360 kb on + strand, within DZA65_RS14320 at 3126.360 kb on + strand, within DZA65_RS14320 at 3126.360 kb on + strand, within DZA65_RS14320 at 3126.360 kb on + strand, within DZA65_RS14320 at 3126.361 kb on - strand, within DZA65_RS14320 at 3126.432 kb on + strand, within DZA65_RS14320 at 3126.433 kb on - strand, within DZA65_RS14320 at 3126.473 kb on + strand, within DZA65_RS14320 at 3126.473 kb on + strand, within DZA65_RS14320 at 3126.473 kb on + strand, within DZA65_RS14320 at 3126.473 kb on + strand, within DZA65_RS14320 at 3126.492 kb on + strand, within DZA65_RS14320 at 3126.514 kb on + strand, within DZA65_RS14320 at 3126.515 kb on - strand, within DZA65_RS14320 at 3126.633 kb on - strand, within DZA65_RS14320
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 3,124,609 + DZA65_RS14305 0.82 +1.4 3,124,609 + DZA65_RS14305 0.82 -0.9 3,124,610 - DZA65_RS14305 0.82 +2.0 3,124,625 + DZA65_RS14305 0.84 -0.4 3,124,625 + DZA65_RS14305 0.84 +0.4 3,124,625 + DZA65_RS14305 0.84 +0.2 3,124,625 + DZA65_RS14305 0.84 +1.9 3,124,626 - DZA65_RS14305 0.84 -1.9 3,124,626 - DZA65_RS14305 0.84 +0.0 3,124,626 - DZA65_RS14305 0.84 -0.4 3,124,673 + DZA65_RS14305 0.87 +0.6 3,124,674 - DZA65_RS14305 0.87 +0.8 3,124,674 - DZA65_RS14305 0.87 -1.2 3,124,692 + DZA65_RS14305 0.88 +1.6 3,124,692 + DZA65_RS14305 0.88 +0.4 3,124,692 + DZA65_RS14305 0.88 -0.8 3,124,692 + DZA65_RS14305 0.88 -0.1 3,124,692 + DZA65_RS14305 0.88 -0.3 3,124,692 + DZA65_RS14305 0.88 -0.6 3,124,695 + DZA65_RS14305 0.89 -0.1 3,124,695 + DZA65_RS14305 0.89 -1.1 3,124,695 + DZA65_RS14305 0.89 -0.1 3,124,695 + DZA65_RS14305 0.89 +0.6 3,124,696 - DZA65_RS14305 0.89 -0.3 3,124,696 - DZA65_RS14305 0.89 +0.2 3,124,696 - DZA65_RS14305 0.89 -0.6 3,124,696 - DZA65_RS14305 0.89 +0.1 3,124,708 - DZA65_RS14305 0.89 -0.2 3,124,738 - +0.9 3,124,759 + +1.6 3,124,780 + -0.2 3,124,823 - +0.7 3,124,858 + -1.9 3,124,858 + -0.9 3,124,858 + +0.8 3,124,858 + -0.3 3,124,858 + -0.6 3,124,858 + -0.1 3,124,858 + +0.4 3,124,858 + -1.8 3,124,859 - +0.8 3,124,859 - -0.2 3,124,859 - -0.9 3,124,859 - +1.7 3,124,859 - -1.8 3,124,859 - -1.6 3,124,859 - +2.3 3,124,882 - -0.2 3,124,892 - +0.6 3,124,892 - +1.0 3,124,892 - -1.8 3,124,924 - -0.1 3,124,924 - -0.6 3,125,014 + DZA65_RS14310 0.31 -0.6 3,125,047 + DZA65_RS14310 0.43 -0.2 3,125,048 - DZA65_RS14310 0.44 -2.2 3,125,119 + DZA65_RS14310 0.70 -0.7 3,125,175 + -0.6 3,125,176 - +0.8 3,125,176 - -0.2 3,125,176 - +1.1 3,125,201 + -1.2 3,125,202 - +0.1 3,125,212 + -0.3 3,125,216 + +0.4 3,125,217 - -0.1 3,125,286 - +0.2 3,125,301 - -0.6 3,125,304 + -0.3 3,125,304 + +0.4 3,125,332 - -0.5 3,125,337 + +0.9 3,125,337 + +1.0 3,125,337 + +0.2 3,125,338 - +0.4 3,125,340 + -1.4 3,125,340 + -0.6 3,125,340 + +1.4 3,125,340 + +0.1 3,125,340 + -0.2 3,125,340 + -0.3 3,125,340 + +0.6 3,125,341 - -1.8 3,125,341 - -0.2 3,125,411 + -0.6 3,125,478 + -3.1 3,125,478 + +1.4 3,125,521 + -1.9 3,125,559 + -0.6 3,125,576 - +0.7 3,125,602 - DZA65_RS14315 0.26 -0.2 3,125,625 - DZA65_RS14315 0.57 -0.3 3,125,692 + +0.6 3,125,714 - +0.2 3,125,818 + +1.2 3,125,818 + -0.6 3,125,818 + -1.4 3,125,818 + +0.4 3,125,818 + -0.1 3,125,818 + +0.7 3,125,818 + -0.9 3,125,818 + -0.2 3,125,818 + +0.1 3,125,818 + +1.4 3,125,818 + -0.0 3,125,818 + -0.6 3,125,818 + +0.5 3,125,819 - -0.2 3,125,819 - +0.7 3,125,819 - -0.9 3,125,819 - -0.6 3,125,819 - +1.0 3,125,819 - +0.0 3,125,819 - +1.0 3,125,819 - -1.7 3,125,851 - -0.9 3,125,864 - -0.5 3,125,877 + +1.4 3,125,897 + +0.4 3,125,897 + +1.8 3,125,898 - +0.4 3,125,914 + +0.8 3,125,914 + +0.1 3,125,942 + +0.3 3,125,942 + +0.1 3,125,943 - +0.7 3,125,943 - -0.6 3,125,943 - +1.4 3,125,943 - +0.4 3,126,052 + DZA65_RS14320 0.18 -0.6 3,126,104 - DZA65_RS14320 0.22 +0.1 3,126,104 - DZA65_RS14320 0.22 -0.5 3,126,199 + DZA65_RS14320 0.30 +1.0 3,126,199 + DZA65_RS14320 0.30 -0.6 3,126,199 + DZA65_RS14320 0.30 -0.3 3,126,199 + DZA65_RS14320 0.30 -0.6 3,126,199 + DZA65_RS14320 0.30 -1.8 3,126,200 - DZA65_RS14320 0.30 -0.2 3,126,200 - DZA65_RS14320 0.30 +1.4 3,126,200 - DZA65_RS14320 0.30 -1.6 3,126,200 - DZA65_RS14320 0.30 -0.4 3,126,200 - DZA65_RS14320 0.30 -0.6 3,126,319 + DZA65_RS14320 0.39 +0.2 3,126,319 + DZA65_RS14320 0.39 +1.4 3,126,319 + DZA65_RS14320 0.39 +1.2 3,126,319 + DZA65_RS14320 0.39 +0.2 3,126,319 + DZA65_RS14320 0.39 -0.3 3,126,319 + DZA65_RS14320 0.39 +0.0 3,126,319 + DZA65_RS14320 0.39 -0.8 3,126,319 + DZA65_RS14320 0.39 -0.9 3,126,319 + DZA65_RS14320 0.39 -0.6 3,126,319 + DZA65_RS14320 0.39 -1.2 3,126,319 + DZA65_RS14320 0.39 +0.1 3,126,319 + DZA65_RS14320 0.39 -2.1 3,126,319 + DZA65_RS14320 0.39 +0.1 3,126,319 + DZA65_RS14320 0.39 -0.0 3,126,319 + DZA65_RS14320 0.39 +0.4 3,126,319 + DZA65_RS14320 0.39 -0.5 3,126,319 + DZA65_RS14320 0.39 -1.3 3,126,319 + DZA65_RS14320 0.39 -0.6 3,126,319 + DZA65_RS14320 0.39 -0.2 3,126,319 + DZA65_RS14320 0.39 -0.1 3,126,319 + DZA65_RS14320 0.39 +1.1 3,126,319 + DZA65_RS14320 0.39 -0.6 3,126,320 - DZA65_RS14320 0.39 -0.3 3,126,320 - DZA65_RS14320 0.39 -0.2 3,126,320 - DZA65_RS14320 0.39 -0.6 3,126,320 - DZA65_RS14320 0.39 +0.1 3,126,320 - DZA65_RS14320 0.39 -1.6 3,126,320 - DZA65_RS14320 0.39 +1.4 3,126,320 - DZA65_RS14320 0.39 -1.9 3,126,320 - DZA65_RS14320 0.39 -0.8 3,126,320 - DZA65_RS14320 0.39 -0.9 3,126,320 - DZA65_RS14320 0.39 -0.1 3,126,320 - DZA65_RS14320 0.39 +0.8 3,126,320 - DZA65_RS14320 0.39 +0.1 3,126,320 - DZA65_RS14320 0.39 +2.0 3,126,320 - DZA65_RS14320 0.39 +0.4 3,126,320 - DZA65_RS14320 0.39 -1.2 3,126,320 - DZA65_RS14320 0.39 +0.2 3,126,320 - DZA65_RS14320 0.39 +0.3 3,126,320 - DZA65_RS14320 0.39 +0.4 3,126,320 - DZA65_RS14320 0.39 -0.8 3,126,320 - DZA65_RS14320 0.39 -0.4 3,126,320 - DZA65_RS14320 0.39 +0.4 3,126,320 - DZA65_RS14320 0.39 -0.5 3,126,320 - DZA65_RS14320 0.39 +1.0 3,126,360 + DZA65_RS14320 0.43 +0.6 3,126,360 + DZA65_RS14320 0.43 +0.8 3,126,360 + DZA65_RS14320 0.43 +0.4 3,126,360 + DZA65_RS14320 0.43 +0.4 3,126,360 + DZA65_RS14320 0.43 -1.2 3,126,361 - DZA65_RS14320 0.43 -2.2 3,126,432 + DZA65_RS14320 0.48 +0.3 3,126,433 - DZA65_RS14320 0.48 +0.0 3,126,473 + DZA65_RS14320 0.51 -0.1 3,126,473 + DZA65_RS14320 0.51 -0.4 3,126,473 + DZA65_RS14320 0.51 +0.2 3,126,473 + DZA65_RS14320 0.51 +0.1 3,126,492 + DZA65_RS14320 0.53 +0.1 3,126,514 + DZA65_RS14320 0.55 -0.1 3,126,515 - DZA65_RS14320 0.55 -0.3 3,126,633 - DZA65_RS14320 0.64 -0.0
Or see this region's nucleotide sequence