Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10055 and DZA65_RS10060 are separated by 4 nucleotides DZA65_RS10060 and DZA65_RS10065 are separated by 161 nucleotides DZA65_RS10065 and DZA65_RS10070 are separated by 259 nucleotides
DZA65_RS10055: DZA65_RS10055 - histidinol dehydrogenase, at 2,128,086 to 2,129,396
_RS10055
DZA65_RS10060: DZA65_RS10060 - ATP phosphoribosyltransferase, at 2,129,401 to 2,130,300
_RS10060
DZA65_RS10065: DZA65_RS10065 - his operon leader peptide, at 2,130,462 to 2,130,509
_RS10065
DZA65_RS10070: DZA65_RS10070 - SDR family oxidoreductase, at 2,130,769 to 2,131,590
_RS10070
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.753 kb on + strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2128.754 kb on - strand, within DZA65_RS10055 at 2129.000 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.002 kb on + strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.003 kb on - strand, within DZA65_RS10055 at 2129.402 kb on + strand at 2129.436 kb on + strand at 2129.436 kb on + strand at 2129.437 kb on - strand at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.618 kb on + strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.619 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.736 kb on - strand, within DZA65_RS10060 at 2129.802 kb on + strand, within DZA65_RS10060 at 2129.803 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.889 kb on - strand, within DZA65_RS10060 at 2129.928 kb on + strand, within DZA65_RS10060 at 2129.954 kb on + strand, within DZA65_RS10060 at 2129.955 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.024 kb on - strand, within DZA65_RS10060 at 2130.280 kb on + strand at 2130.280 kb on + strand at 2130.281 kb on - strand at 2130.291 kb on + strand at 2130.292 kb on - strand at 2130.314 kb on + strand at 2130.335 kb on + strand at 2130.449 kb on - strand at 2130.535 kb on - strand at 2130.535 kb on - strand at 2130.607 kb on - strand at 2130.615 kb on + strand at 2130.616 kb on - strand at 2130.616 kb on - strand at 2130.623 kb on - strand at 2130.631 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.652 kb on + strand at 2130.653 kb on - strand at 2130.654 kb on + strand at 2130.655 kb on - strand at 2130.655 kb on - strand at 2130.664 kb on - strand at 2130.664 kb on - strand at 2130.664 kb on - strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.668 kb on + strand at 2130.669 kb on - strand at 2130.669 kb on - strand at 2130.669 kb on - strand at 2130.670 kb on + strand at 2130.670 kb on + strand at 2130.703 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.705 kb on + strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.706 kb on - strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.709 kb on + strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.710 kb on - strand at 2130.780 kb on - strand at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.030 kb on + strand, within DZA65_RS10070 at 2131.031 kb on - strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.089 kb on + strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.090 kb on - strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.093 kb on + strand, within DZA65_RS10070 at 2131.094 kb on - strand, within DZA65_RS10070 at 2131.115 kb on - strand, within DZA65_RS10070 at 2131.154 kb on + strand, within DZA65_RS10070 at 2131.233 kb on + strand, within DZA65_RS10070 at 2131.233 kb on + strand, within DZA65_RS10070 at 2131.234 kb on - strand, within DZA65_RS10070
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 2,128,753 + DZA65_RS10055 0.51 -3.1 2,128,753 + -1.4 2,128,753 + DZA65_RS10055 0.51 -2.6 2,128,753 + DZA65_RS10055 0.51 -2.6 2,128,753 + DZA65_RS10055 0.51 -3.1 2,128,754 - DZA65_RS10055 0.51 -3.0 2,128,754 - DZA65_RS10055 0.51 +0.2 2,129,000 + DZA65_RS10055 0.70 +1.2 2,129,002 + DZA65_RS10055 0.70 -3.1 2,129,002 + DZA65_RS10055 0.70 -2.3 2,129,002 + DZA65_RS10055 0.70 -0.6 2,129,003 - DZA65_RS10055 0.70 +0.2 2,129,003 - DZA65_RS10055 0.70 -1.8 2,129,402 + -2.1 2,129,436 + -2.9 2,129,436 + -1.1 2,129,437 - -0.8 2,129,618 + DZA65_RS10060 0.24 -1.4 2,129,618 + DZA65_RS10060 0.24 -1.8 2,129,619 - DZA65_RS10060 0.24 -0.1 2,129,619 - DZA65_RS10060 0.24 -1.0 2,129,619 - DZA65_RS10060 0.24 -1.0 2,129,619 - DZA65_RS10060 0.24 -0.0 2,129,619 - DZA65_RS10060 0.24 +0.2 2,129,736 - DZA65_RS10060 0.37 -1.5 2,129,736 - DZA65_RS10060 0.37 -2.4 2,129,802 + DZA65_RS10060 0.45 -2.0 2,129,803 - DZA65_RS10060 0.45 -1.8 2,129,889 - DZA65_RS10060 0.54 -2.6 2,129,889 - DZA65_RS10060 0.54 -2.1 2,129,928 + DZA65_RS10060 0.59 -2.0 2,129,954 + DZA65_RS10060 0.61 -1.8 2,129,955 - DZA65_RS10060 0.62 -2.1 2,130,024 - DZA65_RS10060 0.69 -2.6 2,130,024 - DZA65_RS10060 0.69 -2.1 2,130,280 + -1.6 2,130,280 + -2.1 2,130,281 - -0.1 2,130,291 + -1.8 2,130,292 - +0.2 2,130,314 + -1.6 2,130,335 + -4.1 2,130,449 - +0.2 2,130,535 - +0.2 2,130,535 - -0.3 2,130,607 - +0.9 2,130,615 + +0.5 2,130,616 - -1.8 2,130,616 - +0.2 2,130,623 - +1.2 2,130,631 + -0.3 2,130,652 + +0.4 2,130,652 + +0.6 2,130,652 + -0.1 2,130,652 + -0.8 2,130,653 - -1.1 2,130,654 + +1.5 2,130,655 - -0.0 2,130,655 - -0.0 2,130,664 - +0.6 2,130,664 - -0.0 2,130,664 - +2.8 2,130,668 + -0.3 2,130,668 + +0.2 2,130,668 + +1.0 2,130,668 + -0.6 2,130,669 - +0.5 2,130,669 - -0.8 2,130,669 - +0.5 2,130,670 + +0.2 2,130,670 + -0.1 2,130,703 + +1.2 2,130,705 + +0.5 2,130,705 + +1.2 2,130,705 + -1.8 2,130,705 + -0.5 2,130,705 + +0.2 2,130,705 + +0.9 2,130,705 + +0.2 2,130,705 + -0.3 2,130,705 + +0.5 2,130,705 + +0.0 2,130,706 - -0.4 2,130,706 - -0.2 2,130,706 - -2.0 2,130,706 - -0.8 2,130,706 - +1.1 2,130,706 - +0.8 2,130,709 + +0.7 2,130,709 + +0.6 2,130,709 + +0.4 2,130,709 + +0.0 2,130,710 - -3.4 2,130,710 - +0.2 2,130,710 - +0.2 2,130,710 - -0.3 2,130,780 - -0.3 2,131,030 + DZA65_RS10070 0.32 -0.3 2,131,030 + DZA65_RS10070 0.32 -0.7 2,131,030 + DZA65_RS10070 0.32 -0.2 2,131,030 + DZA65_RS10070 0.32 +1.9 2,131,031 - DZA65_RS10070 0.32 +1.3 2,131,089 + DZA65_RS10070 0.39 -0.2 2,131,089 + DZA65_RS10070 0.39 +0.2 2,131,089 + DZA65_RS10070 0.39 +0.5 2,131,089 + DZA65_RS10070 0.39 -0.3 2,131,089 + DZA65_RS10070 0.39 -0.8 2,131,089 + DZA65_RS10070 0.39 +0.5 2,131,090 - DZA65_RS10070 0.39 -1.2 2,131,090 - DZA65_RS10070 0.39 -0.8 2,131,090 - DZA65_RS10070 0.39 -1.3 2,131,090 - DZA65_RS10070 0.39 -0.8 2,131,090 - DZA65_RS10070 0.39 -1.6 2,131,090 - DZA65_RS10070 0.39 +1.2 2,131,090 - DZA65_RS10070 0.39 +0.5 2,131,093 + DZA65_RS10070 0.39 +1.2 2,131,093 + DZA65_RS10070 0.39 -0.3 2,131,093 + DZA65_RS10070 0.39 +1.2 2,131,093 + DZA65_RS10070 0.39 -0.4 2,131,093 + DZA65_RS10070 0.39 -1.0 2,131,094 - DZA65_RS10070 0.40 +0.5 2,131,115 - DZA65_RS10070 0.42 +0.5 2,131,154 + DZA65_RS10070 0.47 -0.8 2,131,233 + DZA65_RS10070 0.56 -0.2 2,131,233 + DZA65_RS10070 0.56 -0.6 2,131,234 - DZA65_RS10070 0.57 -1.7
Or see this region's nucleotide sequence