Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS10035 and DZA65_RS10040 are separated by 6 nucleotides DZA65_RS10040 and DZA65_RS10045 overlap by 1 nucleotides DZA65_RS10045 and DZA65_RS10050 are separated by 14 nucleotides
DZA65_RS10035: DZA65_RS10035 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,124,603 to 2,125,340
_RS10035
DZA65_RS10040: DZA65_RS10040 - imidazole glycerol phosphate synthase subunit HisH, at 2,125,347 to 2,125,937
_RS10040
DZA65_RS10045: DZA65_RS10045 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,125,937 to 2,127,004
_RS10045
DZA65_RS10050: DZA65_RS10050 - histidinol-phosphate transaminase, at 2,127,019 to 2,128,089
_RS10050
Position (kb)
2125
2126
2127
2128 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2125.293 kb on + strand at 2125.348 kb on + strand at 2125.371 kb on + strand at 2125.885 kb on + strand at 2125.886 kb on - strand at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.163 kb on + strand, within DZA65_RS10045 at 2126.164 kb on - strand, within DZA65_RS10045 at 2126.331 kb on + strand, within DZA65_RS10045 at 2126.332 kb on - strand, within DZA65_RS10045 at 2126.545 kb on + strand, within DZA65_RS10045 at 2126.545 kb on + strand, within DZA65_RS10045 at 2126.546 kb on - strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.628 kb on + strand, within DZA65_RS10045 at 2126.629 kb on - strand, within DZA65_RS10045 at 2126.992 kb on - strand at 2126.992 kb on - strand at 2126.992 kb on - strand at 2127.020 kb on + strand at 2127.020 kb on + strand at 2127.021 kb on - strand at 2127.021 kb on - strand at 2127.221 kb on + strand, within DZA65_RS10050 at 2127.222 kb on - strand, within DZA65_RS10050 at 2127.223 kb on + strand, within DZA65_RS10050 at 2127.232 kb on + strand at 2127.232 kb on + strand, within DZA65_RS10050 at 2127.539 kb on + strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.540 kb on - strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.647 kb on + strand, within DZA65_RS10050 at 2127.648 kb on - strand, within DZA65_RS10050 at 2127.762 kb on - strand, within DZA65_RS10050 at 2127.770 kb on + strand, within DZA65_RS10050 at 2127.837 kb on + strand, within DZA65_RS10050 at 2127.838 kb on - strand, within DZA65_RS10050
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 2,125,293 + -2.7 2,125,348 + -2.5 2,125,371 + -1.3 2,125,885 + -1.3 2,125,886 - -2.3 2,126,163 + DZA65_RS10045 0.21 -1.7 2,126,163 + DZA65_RS10045 0.21 -4.0 2,126,163 + DZA65_RS10045 0.21 +0.1 2,126,164 - DZA65_RS10045 0.21 -0.9 2,126,331 + DZA65_RS10045 0.37 -4.1 2,126,332 - DZA65_RS10045 0.37 -0.9 2,126,545 + DZA65_RS10045 0.57 -2.8 2,126,545 + DZA65_RS10045 0.57 -0.9 2,126,546 - DZA65_RS10045 0.57 -1.9 2,126,628 + DZA65_RS10045 0.65 -1.9 2,126,628 + DZA65_RS10045 0.65 -1.7 2,126,628 + DZA65_RS10045 0.65 -2.5 2,126,629 - DZA65_RS10045 0.65 -0.2 2,126,992 - -1.3 2,126,992 - -1.3 2,126,992 - -1.8 2,127,020 + -2.5 2,127,020 + -2.7 2,127,021 - -0.3 2,127,021 - -2.1 2,127,221 + DZA65_RS10050 0.19 -0.9 2,127,222 - DZA65_RS10050 0.19 +0.7 2,127,223 + DZA65_RS10050 0.19 -2.5 2,127,232 + -2.3 2,127,232 + DZA65_RS10050 0.20 +0.7 2,127,539 + DZA65_RS10050 0.49 -1.9 2,127,540 - DZA65_RS10050 0.49 -2.3 2,127,540 - DZA65_RS10050 0.49 -1.7 2,127,647 + DZA65_RS10050 0.59 -1.7 2,127,647 + DZA65_RS10050 0.59 -0.3 2,127,647 + DZA65_RS10050 0.59 -0.9 2,127,648 - DZA65_RS10050 0.59 -0.9 2,127,762 - DZA65_RS10050 0.69 -0.3 2,127,770 + DZA65_RS10050 0.70 -2.4 2,127,837 + DZA65_RS10050 0.76 -1.3 2,127,838 - DZA65_RS10050 0.76 -2.9
Or see this region's nucleotide sequence