Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS07810 and DZA65_RS07815 overlap by 8 nucleotides DZA65_RS07815 and DZA65_RS07820 overlap by 4 nucleotides DZA65_RS07820 and DZA65_RS07825 overlap by 4 nucleotides DZA65_RS07825 and DZA65_RS07830 are separated by 10 nucleotides
DZA65_RS07810: DZA65_RS07810 - hydrogenase maturation peptidase HycI, at 1,629,327 to 1,629,824
_RS07810
DZA65_RS07815: DZA65_RS07815 - HycH family protein, at 1,629,817 to 1,630,215
_RS07815
DZA65_RS07820: DZA65_RS07820 - NADH-quinone oxidoreductase subunit B family protein, at 1,630,212 to 1,631,003
_RS07820
DZA65_RS07825: DZA65_RS07825 - hydrogenase 4 subunit H, at 1,631,000 to 1,631,569
_RS07825
DZA65_RS07830: DZA65_RS07830 - hydrogenase large subunit, at 1,631,580 to 1,633,307
_RS07830
Position (kb)
1630
1631
1632 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1629.243 kb on + strand at 1629.243 kb on + strand at 1629.257 kb on - strand at 1629.257 kb on - strand at 1629.272 kb on + strand at 1629.288 kb on + strand at 1629.289 kb on - strand at 1629.289 kb on - strand at 1629.304 kb on + strand at 1629.304 kb on + strand at 1629.304 kb on + strand at 1629.305 kb on - strand at 1629.305 kb on - strand at 1629.305 kb on - strand at 1629.424 kb on + strand, within DZA65_RS07810 at 1629.424 kb on + strand, within DZA65_RS07810 at 1629.424 kb on + strand, within DZA65_RS07810 at 1629.425 kb on - strand, within DZA65_RS07810 at 1629.425 kb on - strand, within DZA65_RS07810 at 1629.425 kb on - strand, within DZA65_RS07810 at 1629.425 kb on - strand, within DZA65_RS07810 at 1629.499 kb on + strand, within DZA65_RS07810 at 1629.499 kb on + strand, within DZA65_RS07810 at 1629.499 kb on + strand, within DZA65_RS07810 at 1629.499 kb on + strand, within DZA65_RS07810 at 1629.500 kb on - strand, within DZA65_RS07810 at 1629.500 kb on - strand, within DZA65_RS07810 at 1629.500 kb on - strand, within DZA65_RS07810 at 1629.500 kb on - strand, within DZA65_RS07810 at 1629.500 kb on - strand, within DZA65_RS07810 at 1629.521 kb on + strand, within DZA65_RS07810 at 1629.787 kb on + strand at 1629.787 kb on + strand at 1629.787 kb on + strand at 1629.787 kb on + strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.788 kb on - strand at 1629.789 kb on + strand at 1629.790 kb on - strand at 1629.808 kb on - strand at 1629.814 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.845 kb on + strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.846 kb on - strand at 1629.849 kb on + strand at 1629.878 kb on + strand, within DZA65_RS07815 at 1629.879 kb on - strand, within DZA65_RS07815 at 1629.912 kb on + strand, within DZA65_RS07815 at 1629.912 kb on + strand, within DZA65_RS07815 at 1629.912 kb on + strand, within DZA65_RS07815 at 1629.913 kb on - strand, within DZA65_RS07815 at 1629.913 kb on - strand, within DZA65_RS07815 at 1630.052 kb on + strand, within DZA65_RS07815 at 1630.052 kb on + strand, within DZA65_RS07815 at 1630.052 kb on + strand, within DZA65_RS07815 at 1630.127 kb on + strand, within DZA65_RS07815 at 1630.128 kb on - strand, within DZA65_RS07815 at 1630.128 kb on - strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.130 kb on + strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.131 kb on - strand, within DZA65_RS07815 at 1630.156 kb on + strand, within DZA65_RS07815 at 1630.156 kb on + strand, within DZA65_RS07815 at 1630.157 kb on - strand, within DZA65_RS07815 at 1630.157 kb on - strand, within DZA65_RS07815 at 1630.196 kb on + strand at 1630.247 kb on - strand at 1630.294 kb on + strand, within DZA65_RS07820 at 1630.295 kb on - strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.585 kb on + strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.586 kb on - strand, within DZA65_RS07820 at 1630.630 kb on + strand, within DZA65_RS07820 at 1630.631 kb on - strand, within DZA65_RS07820 at 1630.673 kb on - strand, within DZA65_RS07820 at 1630.829 kb on + strand, within DZA65_RS07820 at 1630.830 kb on - strand, within DZA65_RS07820 at 1630.830 kb on - strand, within DZA65_RS07820 at 1630.849 kb on - strand, within DZA65_RS07820 at 1630.849 kb on - strand, within DZA65_RS07820 at 1630.886 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.892 kb on - strand, within DZA65_RS07820 at 1630.955 kb on + strand at 1630.955 kb on + strand at 1630.956 kb on - strand at 1630.956 kb on - strand at 1630.973 kb on - strand at 1630.997 kb on + strand at 1630.997 kb on + strand at 1630.997 kb on + strand at 1630.997 kb on + strand at 1630.998 kb on - strand at 1630.998 kb on - strand at 1630.998 kb on - strand at 1631.129 kb on + strand, within DZA65_RS07825 at 1631.129 kb on + strand, within DZA65_RS07825 at 1631.130 kb on - strand, within DZA65_RS07825 at 1631.130 kb on - strand, within DZA65_RS07825 at 1631.130 kb on - strand, within DZA65_RS07825 at 1631.250 kb on + strand, within DZA65_RS07825 at 1631.251 kb on - strand, within DZA65_RS07825 at 1631.251 kb on - strand, within DZA65_RS07825 at 1631.251 kb on - strand, within DZA65_RS07825 at 1631.251 kb on - strand, within DZA65_RS07825 at 1631.431 kb on + strand, within DZA65_RS07825 at 1631.431 kb on + strand, within DZA65_RS07825 at 1631.432 kb on - strand, within DZA65_RS07825 at 1631.432 kb on - strand, within DZA65_RS07825 at 1631.432 kb on - strand, within DZA65_RS07825 at 1631.466 kb on + strand, within DZA65_RS07825 at 1631.467 kb on - strand, within DZA65_RS07825 at 1631.517 kb on + strand at 1631.517 kb on + strand at 1631.563 kb on + strand at 1631.563 kb on + strand at 1631.563 kb on + strand at 1631.563 kb on + strand at 1631.564 kb on - strand at 1631.564 kb on - strand at 1631.564 kb on - strand at 1631.564 kb on - strand at 1631.564 kb on - strand at 1631.688 kb on - strand at 1631.695 kb on + strand at 1631.695 kb on + strand at 1631.695 kb on + strand at 1631.756 kb on + strand, within DZA65_RS07830 at 1631.756 kb on + strand, within DZA65_RS07830 at 1631.757 kb on - strand, within DZA65_RS07830 at 1631.757 kb on - strand, within DZA65_RS07830 at 1631.757 kb on - strand, within DZA65_RS07830 at 1631.757 kb on - strand, within DZA65_RS07830 at 1631.782 kb on + strand, within DZA65_RS07830 at 1631.783 kb on - strand, within DZA65_RS07830 at 1631.877 kb on + strand, within DZA65_RS07830 at 1631.877 kb on + strand, within DZA65_RS07830 at 1631.878 kb on - strand, within DZA65_RS07830 at 1631.899 kb on + strand, within DZA65_RS07830
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 1,629,243 + -1.7 1,629,243 + -0.6 1,629,257 - -1.6 1,629,257 - +0.5 1,629,272 + -0.0 1,629,288 + -0.5 1,629,289 - +0.9 1,629,289 - +2.2 1,629,304 + +0.1 1,629,304 + -0.5 1,629,304 + -0.2 1,629,305 - -1.2 1,629,305 - -1.0 1,629,305 - -0.0 1,629,424 + DZA65_RS07810 0.19 -0.8 1,629,424 + DZA65_RS07810 0.19 -0.2 1,629,424 + DZA65_RS07810 0.19 -1.3 1,629,425 - DZA65_RS07810 0.20 -0.3 1,629,425 - DZA65_RS07810 0.20 -2.6 1,629,425 - DZA65_RS07810 0.20 -0.9 1,629,425 - DZA65_RS07810 0.20 +2.8 1,629,499 + DZA65_RS07810 0.35 -2.6 1,629,499 + DZA65_RS07810 0.35 +0.8 1,629,499 + DZA65_RS07810 0.35 +0.4 1,629,499 + DZA65_RS07810 0.35 -2.0 1,629,500 - DZA65_RS07810 0.35 -1.0 1,629,500 - DZA65_RS07810 0.35 -0.2 1,629,500 - DZA65_RS07810 0.35 -1.1 1,629,500 - DZA65_RS07810 0.35 -1.6 1,629,500 - DZA65_RS07810 0.35 +0.8 1,629,521 + DZA65_RS07810 0.39 +0.8 1,629,787 + +1.8 1,629,787 + -2.2 1,629,787 + +1.0 1,629,787 + -0.6 1,629,788 - -0.0 1,629,788 - -0.6 1,629,788 - +0.8 1,629,788 - +1.8 1,629,788 - -0.6 1,629,788 - +0.2 1,629,788 - +0.5 1,629,788 - -1.6 1,629,789 + -0.8 1,629,790 - -0.2 1,629,808 - +0.0 1,629,814 + +0.1 1,629,845 + -0.6 1,629,845 + -0.2 1,629,845 + +2.3 1,629,845 + +0.0 1,629,845 + -1.0 1,629,845 + -1.0 1,629,845 + -1.1 1,629,846 - -0.6 1,629,846 - -1.4 1,629,846 - +0.8 1,629,846 - -0.2 1,629,846 - -1.6 1,629,846 - +0.5 1,629,846 - -0.6 1,629,849 + -1.4 1,629,878 + DZA65_RS07815 0.15 -1.0 1,629,879 - DZA65_RS07815 0.16 +0.7 1,629,912 + DZA65_RS07815 0.24 +1.1 1,629,912 + DZA65_RS07815 0.24 -2.7 1,629,912 + DZA65_RS07815 0.24 -1.0 1,629,913 - DZA65_RS07815 0.24 +0.2 1,629,913 - DZA65_RS07815 0.24 -0.6 1,630,052 + DZA65_RS07815 0.59 +0.0 1,630,052 + DZA65_RS07815 0.59 -0.8 1,630,052 + DZA65_RS07815 0.59 -0.0 1,630,127 + DZA65_RS07815 0.78 -0.6 1,630,128 - DZA65_RS07815 0.78 -0.4 1,630,128 - DZA65_RS07815 0.78 -0.1 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + DZA65_RS07815 0.78 +1.8 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + DZA65_RS07815 0.78 -2.7 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + +0.8 1,630,130 + DZA65_RS07815 0.78 -0.2 1,630,130 + DZA65_RS07815 0.78 -0.4 1,630,130 + DZA65_RS07815 0.78 +1.0 1,630,130 + DZA65_RS07815 0.78 -2.0 1,630,130 + DZA65_RS07815 0.78 +0.8 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + DZA65_RS07815 0.78 -0.8 1,630,130 + DZA65_RS07815 0.78 -0.1 1,630,130 + DZA65_RS07815 0.78 -0.6 1,630,130 + DZA65_RS07815 0.78 +0.3 1,630,130 + DZA65_RS07815 0.78 -0.7 1,630,130 + DZA65_RS07815 0.78 -0.2 1,630,130 + DZA65_RS07815 0.78 +1.8 1,630,130 + DZA65_RS07815 0.78 +0.6 1,630,130 + DZA65_RS07815 0.78 -0.3 1,630,130 + DZA65_RS07815 0.78 -0.2 1,630,130 + DZA65_RS07815 0.78 -1.8 1,630,130 + DZA65_RS07815 0.78 +1.8 1,630,131 - DZA65_RS07815 0.79 +1.1 1,630,131 - DZA65_RS07815 0.79 -0.2 1,630,131 - DZA65_RS07815 0.79 +0.8 1,630,131 - DZA65_RS07815 0.79 -0.2 1,630,131 - DZA65_RS07815 0.79 +1.5 1,630,131 - DZA65_RS07815 0.79 -0.5 1,630,131 - DZA65_RS07815 0.79 -1.2 1,630,131 - DZA65_RS07815 0.79 -0.2 1,630,131 - DZA65_RS07815 0.79 -0.3 1,630,131 - DZA65_RS07815 0.79 +0.4 1,630,131 - DZA65_RS07815 0.79 +1.0 1,630,131 - DZA65_RS07815 0.79 +0.8 1,630,156 + DZA65_RS07815 0.85 +0.3 1,630,156 + DZA65_RS07815 0.85 +0.3 1,630,157 - DZA65_RS07815 0.85 +1.8 1,630,157 - DZA65_RS07815 0.85 -0.0 1,630,196 + +0.3 1,630,247 - +0.2 1,630,294 + DZA65_RS07820 0.10 -0.8 1,630,295 - DZA65_RS07820 0.10 -0.7 1,630,585 + DZA65_RS07820 0.47 +1.8 1,630,585 + DZA65_RS07820 0.47 +0.4 1,630,585 + DZA65_RS07820 0.47 -1.2 1,630,585 + DZA65_RS07820 0.47 -0.8 1,630,585 + DZA65_RS07820 0.47 +1.5 1,630,585 + DZA65_RS07820 0.47 +0.5 1,630,585 + DZA65_RS07820 0.47 +0.5 1,630,586 - DZA65_RS07820 0.47 -0.8 1,630,586 - DZA65_RS07820 0.47 -1.8 1,630,586 - DZA65_RS07820 0.47 -0.6 1,630,586 - DZA65_RS07820 0.47 -1.8 1,630,586 - DZA65_RS07820 0.47 +1.0 1,630,586 - DZA65_RS07820 0.47 +0.8 1,630,586 - DZA65_RS07820 0.47 +0.2 1,630,586 - DZA65_RS07820 0.47 -0.2 1,630,586 - DZA65_RS07820 0.47 +1.8 1,630,586 - DZA65_RS07820 0.47 +0.0 1,630,586 - DZA65_RS07820 0.47 +0.6 1,630,586 - DZA65_RS07820 0.47 +1.8 1,630,586 - DZA65_RS07820 0.47 +1.8 1,630,586 - DZA65_RS07820 0.47 +0.1 1,630,630 + DZA65_RS07820 0.53 +1.4 1,630,631 - DZA65_RS07820 0.53 +0.4 1,630,673 - DZA65_RS07820 0.58 +0.0 1,630,829 + DZA65_RS07820 0.78 +1.1 1,630,830 - DZA65_RS07820 0.78 -1.6 1,630,830 - DZA65_RS07820 0.78 -0.3 1,630,849 - DZA65_RS07820 0.80 -0.4 1,630,849 - DZA65_RS07820 0.80 -0.8 1,630,886 - DZA65_RS07820 0.85 +1.0 1,630,892 - DZA65_RS07820 0.86 -0.6 1,630,892 - DZA65_RS07820 0.86 -0.5 1,630,892 - DZA65_RS07820 0.86 +0.8 1,630,892 - DZA65_RS07820 0.86 +1.8 1,630,892 - DZA65_RS07820 0.86 +0.2 1,630,892 - DZA65_RS07820 0.86 +0.2 1,630,955 + +0.8 1,630,955 + -1.4 1,630,956 - -0.6 1,630,956 - +0.0 1,630,973 - +0.1 1,630,997 + -0.8 1,630,997 + +0.2 1,630,997 + +0.8 1,630,997 + -0.8 1,630,998 - -0.6 1,630,998 - -1.6 1,630,998 - -0.6 1,631,129 + DZA65_RS07825 0.23 -0.8 1,631,129 + DZA65_RS07825 0.23 -0.2 1,631,130 - DZA65_RS07825 0.23 -0.8 1,631,130 - DZA65_RS07825 0.23 -1.2 1,631,130 - DZA65_RS07825 0.23 -0.8 1,631,250 + DZA65_RS07825 0.44 -1.0 1,631,251 - DZA65_RS07825 0.44 +2.6 1,631,251 - DZA65_RS07825 0.44 -1.0 1,631,251 - DZA65_RS07825 0.44 -0.2 1,631,251 - DZA65_RS07825 0.44 -0.5 1,631,431 + DZA65_RS07825 0.76 -1.4 1,631,431 + DZA65_RS07825 0.76 +0.4 1,631,432 - DZA65_RS07825 0.76 +0.4 1,631,432 - DZA65_RS07825 0.76 +1.0 1,631,432 - DZA65_RS07825 0.76 +0.5 1,631,466 + DZA65_RS07825 0.82 -2.0 1,631,467 - DZA65_RS07825 0.82 -1.2 1,631,517 + +1.8 1,631,517 + +0.6 1,631,563 + -1.8 1,631,563 + -0.2 1,631,563 + -0.9 1,631,563 + -1.2 1,631,564 - +2.4 1,631,564 - -0.2 1,631,564 - -0.2 1,631,564 - -0.6 1,631,564 - -0.5 1,631,688 - -0.6 1,631,695 + +2.2 1,631,695 + +1.1 1,631,695 + -0.4 1,631,756 + DZA65_RS07830 0.10 -2.0 1,631,756 + DZA65_RS07830 0.10 +1.8 1,631,757 - DZA65_RS07830 0.10 +0.8 1,631,757 - DZA65_RS07830 0.10 -1.2 1,631,757 - DZA65_RS07830 0.10 -0.2 1,631,757 - DZA65_RS07830 0.10 +1.0 1,631,782 + DZA65_RS07830 0.12 +0.4 1,631,783 - DZA65_RS07830 0.12 -1.1 1,631,877 + DZA65_RS07830 0.17 -1.0 1,631,877 + DZA65_RS07830 0.17 +0.6 1,631,878 - DZA65_RS07830 0.17 +0.8 1,631,899 + DZA65_RS07830 0.18 -1.5
Or see this region's nucleotide sequence