Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS19420 and DZA65_RS22985 are separated by 68 nucleotides DZA65_RS22985 and DZA65_RS19430 are separated by 118 nucleotides DZA65_RS19430 and DZA65_RS19435 are separated by 184 nucleotides
DZA65_RS19420: DZA65_RS19420 - bifunctional riboflavin kinase/FAD synthetase, at 4,201,987 to 4,202,928
_RS19420
DZA65_RS22985: DZA65_RS22985 - hypothetical protein, at 4,202,997 to 4,203,206
_RS22985
DZA65_RS19430: DZA65_RS19430 - 30S ribosomal protein S20, at 4,203,325 to 4,203,588
_RS19430
DZA65_RS19435: DZA65_RS19435 - Na+/H+ antiporter NhaA, at 4,203,773 to 4,204,948
_RS19435
Position (kb)
4202
4203
4204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4202.929 kb on - strand at 4202.929 kb on - strand at 4202.963 kb on - strand at 4202.963 kb on - strand at 4202.963 kb on - strand at 4202.963 kb on - strand at 4202.963 kb on - strand at 4202.969 kb on + strand at 4202.970 kb on - strand at 4203.054 kb on + strand, within DZA65_RS22985 at 4203.055 kb on - strand, within DZA65_RS22985 at 4203.057 kb on - strand, within DZA65_RS22985 at 4203.131 kb on - strand, within DZA65_RS22985 at 4203.143 kb on + strand, within DZA65_RS22985 at 4203.711 kb on - strand at 4203.714 kb on - strand at 4203.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 4,202,929 - +0.2 4,202,929 - -0.8 4,202,963 - +2.8 4,202,963 - -0.2 4,202,963 - -2.5 4,202,963 - +0.0 4,202,963 - -0.1 4,202,969 + -0.6 4,202,970 - +1.0 4,203,054 + DZA65_RS22985 0.27 -0.2 4,203,055 - DZA65_RS22985 0.28 -1.3 4,203,057 - DZA65_RS22985 0.29 -1.1 4,203,131 - DZA65_RS22985 0.64 -0.5 4,203,143 + DZA65_RS22985 0.70 -1.2 4,203,711 - +1.4 4,203,714 - +0.8 4,203,742 - -3.0
Or see this region's nucleotide sequence