Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DZA65_RS13390 and DZA65_RS13395 are separated by 323 nucleotides DZA65_RS13395 and DZA65_RS13400 are separated by 30 nucleotides
DZA65_RS13390: DZA65_RS13390 - quinone-dependent dihydroorotate dehydrogenase, at 2,870,072 to 2,871,079
_RS13390
DZA65_RS13395: DZA65_RS13395 - NADPH-dependent FMN reductase, at 2,871,403 to 2,871,957
_RS13395
DZA65_RS13400: DZA65_RS13400 - sulfonate ABC transporter substrate-binding protein, at 2,871,988 to 2,872,947
_RS13400
Position (kb)
2871
2872 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2870.451 kb on + strand, within DZA65_RS13390 at 2870.451 kb on + strand, within DZA65_RS13390 at 2870.566 kb on + strand, within DZA65_RS13390 at 2870.566 kb on + strand, within DZA65_RS13390 at 2870.578 kb on + strand, within DZA65_RS13390 at 2870.579 kb on - strand, within DZA65_RS13390 at 2870.579 kb on - strand, within DZA65_RS13390 at 2870.595 kb on + strand, within DZA65_RS13390 at 2870.595 kb on + strand, within DZA65_RS13390 at 2870.595 kb on + strand, within DZA65_RS13390 at 2870.595 kb on + strand, within DZA65_RS13390 at 2870.602 kb on + strand, within DZA65_RS13390 at 2870.608 kb on + strand, within DZA65_RS13390 at 2870.608 kb on + strand, within DZA65_RS13390 at 2870.608 kb on + strand, within DZA65_RS13390 at 2870.609 kb on - strand, within DZA65_RS13390 at 2870.609 kb on - strand, within DZA65_RS13390 at 2870.609 kb on - strand, within DZA65_RS13390 at 2870.609 kb on - strand, within DZA65_RS13390 at 2870.668 kb on + strand, within DZA65_RS13390 at 2870.669 kb on - strand, within DZA65_RS13390 at 2870.669 kb on - strand, within DZA65_RS13390 at 2870.669 kb on - strand, within DZA65_RS13390 at 2870.839 kb on + strand, within DZA65_RS13390 at 2870.840 kb on - strand, within DZA65_RS13390 at 2870.840 kb on - strand, within DZA65_RS13390 at 2871.020 kb on - strand at 2871.062 kb on - strand at 2871.062 kb on - strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.075 kb on + strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.076 kb on - strand at 2871.116 kb on + strand at 2871.116 kb on + strand at 2871.116 kb on + strand at 2871.117 kb on - strand at 2871.117 kb on - strand at 2871.120 kb on - strand at 2871.193 kb on + strand at 2871.217 kb on - strand at 2871.245 kb on + strand at 2871.245 kb on + strand at 2871.245 kb on + strand at 2871.246 kb on - strand at 2871.246 kb on - strand at 2871.246 kb on - strand at 2871.337 kb on - strand at 2871.378 kb on + strand at 2871.378 kb on + strand at 2871.378 kb on + strand at 2871.379 kb on - strand at 2871.379 kb on - strand at 2871.387 kb on + strand at 2871.387 kb on + strand at 2871.387 kb on + strand at 2871.387 kb on + strand at 2871.388 kb on - strand at 2871.388 kb on - strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.389 kb on + strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.390 kb on - strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.391 kb on + strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.392 kb on - strand at 2871.411 kb on + strand at 2871.411 kb on + strand at 2871.412 kb on - strand at 2871.414 kb on - strand at 2871.458 kb on + strand at 2871.458 kb on + strand at 2871.458 kb on + strand at 2871.459 kb on - strand, within DZA65_RS13395 at 2871.632 kb on - strand, within DZA65_RS13395 at 2871.758 kb on - strand, within DZA65_RS13395 at 2871.766 kb on - strand, within DZA65_RS13395 at 2871.833 kb on + strand, within DZA65_RS13395 at 2871.833 kb on + strand, within DZA65_RS13395 at 2871.833 kb on + strand, within DZA65_RS13395 at 2871.834 kb on - strand, within DZA65_RS13395 at 2871.834 kb on - strand, within DZA65_RS13395 at 2871.834 kb on - strand, within DZA65_RS13395 at 2871.841 kb on + strand, within DZA65_RS13395 at 2871.841 kb on + strand, within DZA65_RS13395 at 2871.841 kb on + strand, within DZA65_RS13395 at 2871.842 kb on - strand, within DZA65_RS13395 at 2871.850 kb on - strand, within DZA65_RS13395 at 2871.850 kb on - strand, within DZA65_RS13395 at 2871.900 kb on + strand, within DZA65_RS13395 at 2871.900 kb on + strand, within DZA65_RS13395 at 2871.901 kb on - strand, within DZA65_RS13395 at 2871.901 kb on - strand, within DZA65_RS13395 at 2871.901 kb on - strand, within DZA65_RS13395 at 2871.968 kb on + strand at 2871.968 kb on + strand at 2871.968 kb on + strand at 2871.969 kb on - strand at 2871.969 kb on - strand at 2871.970 kb on + strand at 2871.970 kb on + strand at 2871.971 kb on - strand at 2871.972 kb on + strand at 2871.972 kb on + strand at 2871.972 kb on + strand at 2871.973 kb on - strand at 2871.973 kb on - strand at 2871.973 kb on - strand at 2871.973 kb on - strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.987 kb on + strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2871.988 kb on - strand at 2872.002 kb on + strand at 2872.022 kb on + strand at 2872.022 kb on + strand at 2872.022 kb on + strand at 2872.022 kb on + strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.023 kb on - strand at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.297 kb on + strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.298 kb on - strand, within DZA65_RS13400 at 2872.300 kb on - strand, within DZA65_RS13400 at 2872.323 kb on + strand, within DZA65_RS13400 at 2872.323 kb on + strand, within DZA65_RS13400 at 2872.323 kb on + strand, within DZA65_RS13400 at 2872.484 kb on - strand, within DZA65_RS13400 at 2872.542 kb on + strand, within DZA65_RS13400 at 2872.542 kb on + strand, within DZA65_RS13400 at 2872.543 kb on - strand, within DZA65_RS13400 at 2872.543 kb on - strand, within DZA65_RS13400 at 2872.543 kb on - strand, within DZA65_RS13400 at 2872.555 kb on + strand, within DZA65_RS13400 at 2872.556 kb on - strand, within DZA65_RS13400 at 2872.558 kb on + strand, within DZA65_RS13400 at 2872.559 kb on - strand, within DZA65_RS13400 at 2872.633 kb on + strand, within DZA65_RS13400 at 2872.634 kb on - strand, within DZA65_RS13400 at 2872.634 kb on - strand, within DZA65_RS13400 at 2872.647 kb on + strand, within DZA65_RS13400 at 2872.647 kb on + strand, within DZA65_RS13400 at 2872.647 kb on + strand, within DZA65_RS13400 at 2872.647 kb on + strand, within DZA65_RS13400 at 2872.648 kb on - strand, within DZA65_RS13400 at 2872.648 kb on - strand, within DZA65_RS13400 at 2872.648 kb on - strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.651 kb on + strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.652 kb on - strand, within DZA65_RS13400 at 2872.663 kb on + strand, within DZA65_RS13400 at 2872.792 kb on + strand, within DZA65_RS13400 at 2872.792 kb on + strand, within DZA65_RS13400 at 2872.793 kb on - strand, within DZA65_RS13400 at 2872.945 kb on + strand at 2872.945 kb on + strand at 2872.945 kb on + strand at 2872.945 kb on + strand at 2872.946 kb on - strand at 2872.946 kb on - strand at 2872.946 kb on - strand at 2872.946 kb on - strand at 2872.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 2,870,451 + DZA65_RS13390 0.38 -2.2 2,870,451 + DZA65_RS13390 0.38 -1.2 2,870,566 + DZA65_RS13390 0.49 -2.2 2,870,566 + DZA65_RS13390 0.49 -2.3 2,870,578 + DZA65_RS13390 0.50 -2.4 2,870,579 - DZA65_RS13390 0.50 -1.2 2,870,579 - DZA65_RS13390 0.50 -3.3 2,870,595 + DZA65_RS13390 0.52 -3.0 2,870,595 + DZA65_RS13390 0.52 -3.1 2,870,595 + DZA65_RS13390 0.52 -4.1 2,870,595 + DZA65_RS13390 0.52 -2.3 2,870,602 + DZA65_RS13390 0.53 -2.0 2,870,608 + DZA65_RS13390 0.53 -3.2 2,870,608 + DZA65_RS13390 0.53 -0.2 2,870,608 + DZA65_RS13390 0.53 -1.7 2,870,609 - DZA65_RS13390 0.53 -3.6 2,870,609 - DZA65_RS13390 0.53 -3.2 2,870,609 - DZA65_RS13390 0.53 -2.3 2,870,609 - DZA65_RS13390 0.53 -3.9 2,870,668 + DZA65_RS13390 0.59 -3.0 2,870,669 - DZA65_RS13390 0.59 -4.9 2,870,669 - DZA65_RS13390 0.59 -3.0 2,870,669 - DZA65_RS13390 0.59 -3.6 2,870,839 + DZA65_RS13390 0.76 -0.2 2,870,840 - DZA65_RS13390 0.76 -3.0 2,870,840 - DZA65_RS13390 0.76 -1.7 2,871,020 - -2.5 2,871,062 - -1.7 2,871,062 - -3.0 2,871,075 + -2.2 2,871,075 + -2.7 2,871,075 + -4.8 2,871,075 + -2.2 2,871,075 + -0.4 2,871,075 + -2.2 2,871,075 + -0.9 2,871,075 + -2.2 2,871,075 + -1.7 2,871,075 + -0.3 2,871,076 - -2.2 2,871,076 - -2.6 2,871,076 - -2.2 2,871,076 - -3.2 2,871,076 - -2.9 2,871,076 - -2.7 2,871,076 - -3.5 2,871,076 - -1.2 2,871,076 - -3.5 2,871,076 - -0.2 2,871,116 + +0.7 2,871,116 + -0.0 2,871,116 + -2.9 2,871,117 - -1.0 2,871,117 - +0.6 2,871,120 - +0.6 2,871,193 + -0.2 2,871,217 - -0.2 2,871,245 + -0.0 2,871,245 + -0.2 2,871,245 + -0.8 2,871,246 - +0.8 2,871,246 - -0.5 2,871,246 - -0.2 2,871,337 - -1.5 2,871,378 + -2.6 2,871,378 + -2.7 2,871,378 + -0.1 2,871,379 - -0.5 2,871,379 - +1.1 2,871,387 + -0.4 2,871,387 + +0.0 2,871,387 + +0.4 2,871,387 + +0.9 2,871,388 - -1.7 2,871,388 - +0.2 2,871,389 + -0.6 2,871,389 + -0.2 2,871,389 + +0.1 2,871,389 + -0.2 2,871,389 + -0.2 2,871,389 + -1.3 2,871,389 + +1.7 2,871,389 + +0.9 2,871,389 + +0.4 2,871,389 + +0.1 2,871,389 + +0.7 2,871,389 + -0.2 2,871,389 + -0.2 2,871,389 + -0.5 2,871,389 + +0.5 2,871,390 - +0.2 2,871,390 - +0.3 2,871,390 - +0.5 2,871,390 - -2.0 2,871,390 - -0.4 2,871,390 - +1.2 2,871,390 - -0.6 2,871,390 - +0.4 2,871,390 - -0.7 2,871,391 + +0.0 2,871,391 + -0.5 2,871,391 + -0.2 2,871,391 + +2.7 2,871,391 + +0.6 2,871,391 + +0.3 2,871,391 + -2.2 2,871,391 + -1.2 2,871,392 - -2.5 2,871,392 - +2.0 2,871,392 - +0.6 2,871,392 - -0.2 2,871,392 - -0.2 2,871,392 - +0.0 2,871,392 - +1.0 2,871,392 - -0.7 2,871,392 - -0.8 2,871,392 - +1.6 2,871,392 - +0.9 2,871,392 - -0.6 2,871,392 - -1.3 2,871,392 - +0.6 2,871,392 - -0.6 2,871,392 - -1.5 2,871,392 - -0.9 2,871,392 - +0.5 2,871,392 - -0.7 2,871,411 + -0.2 2,871,411 + -1.9 2,871,412 - +1.4 2,871,414 - +0.4 2,871,458 + -0.3 2,871,458 + +2.2 2,871,458 + -0.8 2,871,459 - DZA65_RS13395 0.10 +0.7 2,871,632 - DZA65_RS13395 0.41 -2.5 2,871,758 - DZA65_RS13395 0.64 -0.5 2,871,766 - DZA65_RS13395 0.65 +0.2 2,871,833 + DZA65_RS13395 0.77 -0.9 2,871,833 + DZA65_RS13395 0.77 -0.2 2,871,833 + DZA65_RS13395 0.77 +0.0 2,871,834 - DZA65_RS13395 0.78 +1.2 2,871,834 - DZA65_RS13395 0.78 +0.4 2,871,834 - DZA65_RS13395 0.78 +1.0 2,871,841 + DZA65_RS13395 0.79 -2.7 2,871,841 + DZA65_RS13395 0.79 -0.2 2,871,841 + DZA65_RS13395 0.79 -0.4 2,871,842 - DZA65_RS13395 0.79 -1.0 2,871,850 - DZA65_RS13395 0.81 +0.0 2,871,850 - DZA65_RS13395 0.81 -1.7 2,871,900 + DZA65_RS13395 0.90 +0.1 2,871,900 + DZA65_RS13395 0.90 -1.5 2,871,901 - DZA65_RS13395 0.90 +0.2 2,871,901 - DZA65_RS13395 0.90 +2.0 2,871,901 - DZA65_RS13395 0.90 -0.6 2,871,968 + -2.0 2,871,968 + -2.2 2,871,968 + +0.8 2,871,969 - +0.4 2,871,969 - -1.2 2,871,970 + +0.1 2,871,970 + +0.3 2,871,971 - +0.0 2,871,972 + +0.8 2,871,972 + -0.2 2,871,972 + -0.9 2,871,973 - -1.2 2,871,973 - +0.5 2,871,973 - -1.2 2,871,973 - -2.7 2,871,987 + -0.0 2,871,987 + -0.7 2,871,987 + -0.8 2,871,987 + -0.4 2,871,987 + +1.7 2,871,987 + +1.0 2,871,987 + +0.8 2,871,987 + -0.2 2,871,987 + +1.1 2,871,987 + -0.6 2,871,987 + +0.3 2,871,987 + -1.5 2,871,987 + -2.0 2,871,987 + -2.5 2,871,988 - -0.4 2,871,988 - +0.4 2,871,988 - -0.2 2,871,988 - +2.5 2,871,988 - -1.4 2,871,988 - -0.5 2,871,988 - -0.2 2,871,988 - +1.1 2,871,988 - -0.4 2,871,988 - -0.3 2,871,988 - -0.6 2,872,002 + -0.3 2,872,022 + -0.1 2,872,022 + +0.7 2,872,022 + +0.8 2,872,022 + -0.2 2,872,023 - +0.1 2,872,023 - -0.3 2,872,023 - +0.8 2,872,023 - +0.6 2,872,023 - -0.2 2,872,023 - -0.3 2,872,023 - -0.6 2,872,023 - -0.6 2,872,023 - -1.0 2,872,023 - -0.0 2,872,297 + DZA65_RS13400 0.32 -0.7 2,872,297 + DZA65_RS13400 0.32 +2.2 2,872,297 + DZA65_RS13400 0.32 +0.8 2,872,297 + DZA65_RS13400 0.32 -0.9 2,872,297 + DZA65_RS13400 0.32 +0.2 2,872,297 + DZA65_RS13400 0.32 +0.1 2,872,297 + DZA65_RS13400 0.32 +0.7 2,872,298 - DZA65_RS13400 0.32 -0.7 2,872,298 - DZA65_RS13400 0.32 +0.6 2,872,298 - DZA65_RS13400 0.32 +0.5 2,872,298 - DZA65_RS13400 0.32 -0.3 2,872,298 - DZA65_RS13400 0.32 -1.3 2,872,298 - DZA65_RS13400 0.32 -0.7 2,872,300 - DZA65_RS13400 0.33 -0.3 2,872,323 + DZA65_RS13400 0.35 -1.7 2,872,323 + DZA65_RS13400 0.35 -0.7 2,872,323 + DZA65_RS13400 0.35 +1.1 2,872,484 - DZA65_RS13400 0.52 +0.4 2,872,542 + DZA65_RS13400 0.58 -0.7 2,872,542 + DZA65_RS13400 0.58 -1.2 2,872,543 - DZA65_RS13400 0.58 -1.7 2,872,543 - DZA65_RS13400 0.58 +0.2 2,872,543 - DZA65_RS13400 0.58 +0.5 2,872,555 + DZA65_RS13400 0.59 -0.2 2,872,556 - DZA65_RS13400 0.59 -0.2 2,872,558 + DZA65_RS13400 0.59 -0.4 2,872,559 - DZA65_RS13400 0.59 +0.4 2,872,633 + DZA65_RS13400 0.67 -0.9 2,872,634 - DZA65_RS13400 0.67 -1.0 2,872,634 - DZA65_RS13400 0.67 +1.1 2,872,647 + DZA65_RS13400 0.69 -1.4 2,872,647 + DZA65_RS13400 0.69 +0.1 2,872,647 + DZA65_RS13400 0.69 +0.1 2,872,647 + DZA65_RS13400 0.69 -3.2 2,872,648 - DZA65_RS13400 0.69 -0.1 2,872,648 - DZA65_RS13400 0.69 -0.2 2,872,648 - DZA65_RS13400 0.69 +0.2 2,872,651 + DZA65_RS13400 0.69 -0.2 2,872,651 + DZA65_RS13400 0.69 +0.8 2,872,651 + DZA65_RS13400 0.69 -0.7 2,872,651 + DZA65_RS13400 0.69 -0.2 2,872,651 + DZA65_RS13400 0.69 -1.5 2,872,651 + DZA65_RS13400 0.69 +0.5 2,872,651 + DZA65_RS13400 0.69 +0.4 2,872,651 + DZA65_RS13400 0.69 -1.5 2,872,651 + DZA65_RS13400 0.69 -0.9 2,872,651 + DZA65_RS13400 0.69 +0.5 2,872,652 - DZA65_RS13400 0.69 -2.2 2,872,652 - DZA65_RS13400 0.69 +0.8 2,872,652 - DZA65_RS13400 0.69 +0.3 2,872,652 - DZA65_RS13400 0.69 -0.1 2,872,652 - DZA65_RS13400 0.69 -0.6 2,872,652 - DZA65_RS13400 0.69 -1.4 2,872,652 - DZA65_RS13400 0.69 -0.7 2,872,652 - DZA65_RS13400 0.69 +0.3 2,872,652 - DZA65_RS13400 0.69 +0.3 2,872,663 + DZA65_RS13400 0.70 +0.3 2,872,792 + DZA65_RS13400 0.84 -0.5 2,872,792 + DZA65_RS13400 0.84 +0.5 2,872,793 - DZA65_RS13400 0.84 -1.4 2,872,945 + +1.1 2,872,945 + +0.5 2,872,945 + +0.0 2,872,945 + -0.6 2,872,946 - -0.3 2,872,946 - -0.8 2,872,946 - -0.5 2,872,946 - -0.5 2,872,946 - -0.5
Or see this region's nucleotide sequence