Experiment: M9+glucose+arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS01130 and HER17_RS01135 are separated by 118 nucleotides HER17_RS01135 and HER17_RS01140 are separated by 495 nucleotides HER17_RS01140 and HER17_RS01145 are separated by 206 nucleotides
HER17_RS01130: HER17_RS01130 - ATP-dependent RNA helicase RhlB, at 263,418 to 264,707
_RS01130
HER17_RS01135: HER17_RS01135 - thioredoxin TrxA, at 264,826 to 265,152
_RS01135
HER17_RS01140: HER17_RS01140 - transcription termination factor Rho, at 265,648 to 266,907
_RS01140
HER17_RS01145: HER17_RS01145 - UDP-N-acetylglucosamine--undecaprenyl-phosphate N-acetylglucosaminephosphotransferase, at 267,114 to 268,211
_RS01145
Position (kb) 265
266
267 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 264.690 kb on + strand at 264.690 kb on + strand at 264.707 kb on + strand at 264.708 kb on - strand at 264.715 kb on + strand at 264.721 kb on - strand at 264.727 kb on + strand at 264.727 kb on + strand at 264.727 kb on + strand at 264.731 kb on + strand at 264.731 kb on + strand at 264.732 kb on - strand at 264.732 kb on - strand at 264.749 kb on + strand at 264.761 kb on + strand at 264.761 kb on + strand at 264.761 kb on + strand at 264.761 kb on + strand at 264.762 kb on - strand at 264.762 kb on - strand at 264.788 kb on + strand at 264.788 kb on + strand at 264.789 kb on - strand at 264.789 kb on - strand at 264.821 kb on - strand at 264.839 kb on + strand at 264.839 kb on + strand at 264.839 kb on + strand at 264.894 kb on + strand, within HER17_RS01135 at 264.948 kb on + strand, within HER17_RS01135 at 264.948 kb on + strand, within HER17_RS01135 at 265.036 kb on + strand, within HER17_RS01135 at 265.037 kb on - strand, within HER17_RS01135 at 265.056 kb on + strand, within HER17_RS01135 at 265.056 kb on + strand, within HER17_RS01135 at 265.057 kb on - strand, within HER17_RS01135 at 265.057 kb on - strand, within HER17_RS01135 at 265.154 kb on + strand at 265.154 kb on + strand at 265.425 kb on + strand at 265.428 kb on + strand at 265.428 kb on + strand at 265.434 kb on + strand at 265.434 kb on + strand at 265.439 kb on + strand at 265.439 kb on + strand at 265.449 kb on + strand at 265.449 kb on + strand at 265.493 kb on + strand at 265.519 kb on + strand at 265.519 kb on + strand at 265.570 kb on + strand at 265.570 kb on + strand at 265.610 kb on + strand at 265.610 kb on + strand at 265.632 kb on + strand at 266.906 kb on - strand at 266.932 kb on + strand at 266.977 kb on + strand at 266.978 kb on - strand at 266.978 kb on - strand at 266.978 kb on - strand at 266.978 kb on - strand at 267.004 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.062 kb on + strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.063 kb on - strand at 267.064 kb on + strand at 267.064 kb on + strand at 267.065 kb on - strand at 267.065 kb on - strand at 267.065 kb on - strand at 267.065 kb on - strand at 267.065 kb on - strand at 267.065 kb on - strand at 267.082 kb on + strand at 267.082 kb on + strand at 267.082 kb on + strand at 267.083 kb on - strand at 267.095 kb on + strand at 267.096 kb on - strand at 267.121 kb on + strand at 267.121 kb on + strand at 267.128 kb on + strand at 267.143 kb on - strand at 267.228 kb on + strand, within HER17_RS01145 at 267.229 kb on - strand, within HER17_RS01145 at 267.229 kb on - strand, within HER17_RS01145 at 267.269 kb on + strand, within HER17_RS01145 at 267.269 kb on + strand, within HER17_RS01145 at 267.270 kb on - strand, within HER17_RS01145 at 267.270 kb on - strand, within HER17_RS01145 at 267.270 kb on - strand, within HER17_RS01145 at 267.279 kb on + strand, within HER17_RS01145 at 267.296 kb on + strand, within HER17_RS01145 at 267.297 kb on - strand, within HER17_RS01145 at 267.297 kb on - strand, within HER17_RS01145 at 267.300 kb on + strand, within HER17_RS01145 at 267.300 kb on + strand, within HER17_RS01145 at 267.300 kb on + strand, within HER17_RS01145 at 267.300 kb on + strand, within HER17_RS01145 at 267.308 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.318 kb on + strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.319 kb on - strand, within HER17_RS01145 at 267.320 kb on + strand, within HER17_RS01145 at 267.320 kb on + strand, within HER17_RS01145 at 267.320 kb on + strand, within HER17_RS01145 at 267.321 kb on - strand, within HER17_RS01145 at 267.321 kb on - strand, within HER17_RS01145 at 267.321 kb on - strand, within HER17_RS01145 at 267.339 kb on + strand, within HER17_RS01145 at 267.364 kb on + strand, within HER17_RS01145 at 267.365 kb on - strand, within HER17_RS01145 at 267.368 kb on + strand, within HER17_RS01145 at 267.470 kb on - strand, within HER17_RS01145 at 267.573 kb on - strand, within HER17_RS01145 at 267.573 kb on - strand, within HER17_RS01145 at 267.573 kb on - strand, within HER17_RS01145 at 267.646 kb on - strand, within HER17_RS01145 at 267.659 kb on + strand, within HER17_RS01145 at 267.659 kb on + strand, within HER17_RS01145 at 267.660 kb on - strand, within HER17_RS01145 at 267.703 kb on + strand, within HER17_RS01145 at 267.703 kb on + strand, within HER17_RS01145 at 267.704 kb on - strand, within HER17_RS01145 at 267.704 kb on - strand, within HER17_RS01145 at 267.704 kb on - strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.708 kb on + strand, within HER17_RS01145 at 267.709 kb on - strand, within HER17_RS01145 at 267.720 kb on - strand, within HER17_RS01145 at 267.744 kb on + strand, within HER17_RS01145 at 267.744 kb on + strand, within HER17_RS01145 at 267.840 kb on - strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.896 kb on + strand, within HER17_RS01145 at 267.897 kb on - strand, within HER17_RS01145 at 267.897 kb on - strand, within HER17_RS01145 at 267.897 kb on - strand, within HER17_RS01145 at 267.897 kb on - strand, within HER17_RS01145 at 267.900 kb on + strand, within HER17_RS01145 at 267.901 kb on - strand, within HER17_RS01145 at 267.901 kb on - strand, within HER17_RS01145
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glucose+arginine remove 264,690 + -2.4 264,690 + -0.6 264,707 + -0.0 264,708 - -0.8 264,715 + -2.0 264,721 - +1.3 264,727 + +1.7 264,727 + -0.2 264,727 + +0.4 264,731 + -2.8 264,731 + -1.0 264,732 - +0.0 264,732 - +1.3 264,749 + +1.0 264,761 + +0.6 264,761 + -1.6 264,761 + +0.1 264,761 + -0.8 264,762 - -0.3 264,762 - -1.3 264,788 + +1.6 264,788 + +0.8 264,789 - -0.6 264,789 - +0.2 264,821 - -1.5 264,839 + -0.4 264,839 + -1.8 264,839 + -0.4 264,894 + HER17_RS01135 0.21 -0.3 264,948 + HER17_RS01135 0.37 -1.4 264,948 + HER17_RS01135 0.37 -1.3 265,036 + HER17_RS01135 0.64 +0.6 265,037 - HER17_RS01135 0.65 -3.2 265,056 + HER17_RS01135 0.70 -0.5 265,056 + HER17_RS01135 0.70 -0.8 265,057 - HER17_RS01135 0.71 -0.9 265,057 - HER17_RS01135 0.71 -1.4 265,154 + +0.2 265,154 + -0.1 265,425 + -1.5 265,428 + +0.3 265,428 + +0.9 265,434 + -0.0 265,434 + -2.5 265,439 + +0.3 265,439 + -2.2 265,449 + -0.9 265,449 + -0.8 265,493 + +0.8 265,519 + +0.9 265,519 + +0.5 265,570 + +0.2 265,570 + -0.8 265,610 + +1.1 265,610 + +0.8 265,632 + +0.6 266,906 - +0.0 266,932 + +0.4 266,977 + +0.6 266,978 - -0.8 266,978 - -1.7 266,978 - -0.1 266,978 - +0.8 267,004 + -1.3 267,062 + +0.1 267,062 + +0.3 267,062 + -0.1 267,062 + -0.4 267,062 + +0.9 267,062 + -2.7 267,062 + +0.5 267,062 + -1.9 267,063 - +2.1 267,063 - -2.4 267,063 - -1.3 267,063 - -0.5 267,063 - +1.6 267,063 - +0.2 267,063 - +0.2 267,063 - -0.6 267,064 + -0.8 267,064 + -1.0 267,065 - +0.6 267,065 - +1.2 267,065 - +1.3 267,065 - +0.5 267,065 - +1.1 267,065 - -0.2 267,082 + -1.4 267,082 + +0.4 267,082 + +0.4 267,083 - -1.4 267,095 + +1.8 267,096 - -0.8 267,121 + +0.7 267,121 + -2.1 267,128 + -0.2 267,143 - +1.2 267,228 + HER17_RS01145 0.10 -0.6 267,229 - HER17_RS01145 0.10 -2.9 267,229 - HER17_RS01145 0.10 -1.4 267,269 + HER17_RS01145 0.14 +0.4 267,269 + HER17_RS01145 0.14 +1.3 267,270 - HER17_RS01145 0.14 +2.0 267,270 - HER17_RS01145 0.14 -0.9 267,270 - HER17_RS01145 0.14 +0.4 267,279 + HER17_RS01145 0.15 -0.2 267,296 + HER17_RS01145 0.17 +0.4 267,297 - HER17_RS01145 0.17 +1.1 267,297 - HER17_RS01145 0.17 -0.8 267,300 + HER17_RS01145 0.17 -3.4 267,300 + HER17_RS01145 0.17 +0.2 267,300 + HER17_RS01145 0.17 -2.3 267,300 + HER17_RS01145 0.17 +1.1 267,308 + HER17_RS01145 0.18 -1.0 267,318 + HER17_RS01145 0.19 -0.8 267,318 + HER17_RS01145 0.19 +1.0 267,318 + HER17_RS01145 0.19 -0.1 267,318 + HER17_RS01145 0.19 +0.3 267,318 + HER17_RS01145 0.19 -1.1 267,318 + HER17_RS01145 0.19 -1.1 267,318 + HER17_RS01145 0.19 -1.4 267,318 + HER17_RS01145 0.19 -0.8 267,318 + HER17_RS01145 0.19 +0.6 267,318 + HER17_RS01145 0.19 +0.7 267,319 - HER17_RS01145 0.19 +0.5 267,319 - HER17_RS01145 0.19 -0.7 267,319 - HER17_RS01145 0.19 +0.2 267,319 - HER17_RS01145 0.19 +0.3 267,319 - HER17_RS01145 0.19 -0.0 267,319 - HER17_RS01145 0.19 +0.8 267,319 - HER17_RS01145 0.19 +1.1 267,319 - HER17_RS01145 0.19 +0.9 267,319 - HER17_RS01145 0.19 +1.0 267,319 - HER17_RS01145 0.19 -1.4 267,319 - HER17_RS01145 0.19 -2.4 267,319 - HER17_RS01145 0.19 +1.8 267,319 - HER17_RS01145 0.19 -1.9 267,319 - HER17_RS01145 0.19 -0.4 267,319 - HER17_RS01145 0.19 +0.3 267,320 + HER17_RS01145 0.19 +0.6 267,320 + HER17_RS01145 0.19 +0.2 267,320 + HER17_RS01145 0.19 -2.4 267,321 - HER17_RS01145 0.19 -0.1 267,321 - HER17_RS01145 0.19 -1.1 267,321 - HER17_RS01145 0.19 -1.7 267,339 + HER17_RS01145 0.20 -1.6 267,364 + HER17_RS01145 0.23 -1.4 267,365 - HER17_RS01145 0.23 -1.8 267,368 + HER17_RS01145 0.23 -2.4 267,470 - HER17_RS01145 0.32 +0.7 267,573 - HER17_RS01145 0.42 +0.0 267,573 - HER17_RS01145 0.42 +0.6 267,573 - HER17_RS01145 0.42 -0.6 267,646 - HER17_RS01145 0.48 -0.3 267,659 + HER17_RS01145 0.50 +0.2 267,659 + HER17_RS01145 0.50 -1.1 267,660 - HER17_RS01145 0.50 -1.0 267,703 + HER17_RS01145 0.54 -2.0 267,703 + HER17_RS01145 0.54 -0.0 267,704 - HER17_RS01145 0.54 -0.4 267,704 - HER17_RS01145 0.54 +0.1 267,704 - HER17_RS01145 0.54 -1.7 267,708 + HER17_RS01145 0.54 -1.3 267,708 + HER17_RS01145 0.54 +0.9 267,708 + HER17_RS01145 0.54 -1.8 267,708 + HER17_RS01145 0.54 -1.4 267,708 + HER17_RS01145 0.54 +0.6 267,708 + HER17_RS01145 0.54 +2.0 267,709 - HER17_RS01145 0.54 +0.4 267,720 - HER17_RS01145 0.55 +0.2 267,744 + HER17_RS01145 0.57 -2.1 267,744 + HER17_RS01145 0.57 +0.7 267,840 - HER17_RS01145 0.66 +0.6 267,896 + HER17_RS01145 0.71 +1.6 267,896 + HER17_RS01145 0.71 -1.4 267,896 + HER17_RS01145 0.71 -0.3 267,896 + HER17_RS01145 0.71 -0.4 267,896 + HER17_RS01145 0.71 +1.0 267,896 + HER17_RS01145 0.71 +1.4 267,896 + HER17_RS01145 0.71 -0.6 267,897 - HER17_RS01145 0.71 +0.8 267,897 - HER17_RS01145 0.71 +0.2 267,897 - HER17_RS01145 0.71 +1.1 267,897 - HER17_RS01145 0.71 +2.1 267,900 + HER17_RS01145 0.72 -0.7 267,901 - HER17_RS01145 0.72 -1.8 267,901 - HER17_RS01145 0.72 +0.6
Or see this region's nucleotide sequence