Experiment: M9+glucose+arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS17180 and HER17_RS17185 are separated by 148 nucleotides HER17_RS17185 and HER17_RS17190 are separated by 66 nucleotides HER17_RS17190 and HER17_RS17195 overlap by 1 nucleotides HER17_RS17195 and HER17_RS17200 are separated by 250 nucleotides
HER17_RS17180: HER17_RS17180 - phosphoenolpyruvate-protein phosphotransferase PtsI, at 3,867,665 to 3,869,392
_RS17180
HER17_RS17185: HER17_RS17185 - PTS glucose transporter subunit IIA, at 3,869,541 to 3,870,050
_RS17185
HER17_RS17190: HER17_RS17190 - ATP-binding protein, at 3,870,117 to 3,871,484
_RS17190
HER17_RS17195: HER17_RS17195 - response regulator transcription factor, at 3,871,484 to 3,872,164
_RS17195
HER17_RS17200: HER17_RS17200 - efflux RND transporter periplasmic adaptor subunit, at 3,872,415 to 3,873,614
_RS17200
Position (kb)
3870
3871
3872 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3869.197 kb on + strand, within HER17_RS17180 at 3869.197 kb on + strand, within HER17_RS17180 at 3869.198 kb on - strand, within HER17_RS17180 at 3869.198 kb on - strand, within HER17_RS17180 at 3869.202 kb on + strand, within HER17_RS17180 at 3869.202 kb on + strand, within HER17_RS17180 at 3869.202 kb on + strand, within HER17_RS17180 at 3869.288 kb on + strand at 3869.289 kb on - strand at 3869.346 kb on + strand at 3869.369 kb on - strand at 3869.401 kb on + strand at 3869.405 kb on + strand at 3869.406 kb on - strand at 3869.406 kb on - strand at 3869.499 kb on + strand at 3869.499 kb on + strand at 3869.504 kb on + strand at 3869.504 kb on + strand at 3869.507 kb on - strand at 3869.507 kb on - strand at 3869.507 kb on - strand at 3869.520 kb on - strand at 3869.520 kb on - strand at 3869.522 kb on + strand at 3869.529 kb on + strand at 3869.529 kb on + strand at 3869.530 kb on - strand at 3870.006 kb on - strand at 3870.060 kb on + strand at 3870.061 kb on - strand at 3870.061 kb on - strand at 3870.072 kb on - strand at 3870.072 kb on - strand at 3870.074 kb on + strand at 3870.075 kb on - strand at 3870.075 kb on - strand at 3870.154 kb on - strand at 3870.186 kb on - strand at 3870.186 kb on - strand at 3870.238 kb on + strand at 3870.288 kb on - strand, within HER17_RS17190 at 3870.299 kb on + strand, within HER17_RS17190 at 3870.299 kb on + strand, within HER17_RS17190 at 3870.403 kb on - strand, within HER17_RS17190 at 3870.403 kb on - strand, within HER17_RS17190 at 3870.524 kb on - strand, within HER17_RS17190 at 3870.572 kb on - strand, within HER17_RS17190 at 3870.585 kb on - strand, within HER17_RS17190 at 3870.632 kb on + strand, within HER17_RS17190 at 3870.632 kb on + strand, within HER17_RS17190 at 3870.632 kb on + strand, within HER17_RS17190 at 3870.633 kb on - strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.678 kb on + strand, within HER17_RS17190 at 3870.679 kb on - strand, within HER17_RS17190 at 3870.679 kb on - strand, within HER17_RS17190 at 3870.679 kb on - strand, within HER17_RS17190 at 3870.680 kb on + strand, within HER17_RS17190 at 3870.680 kb on + strand, within HER17_RS17190 at 3870.680 kb on + strand, within HER17_RS17190 at 3870.680 kb on + strand, within HER17_RS17190 at 3870.681 kb on - strand, within HER17_RS17190 at 3870.681 kb on - strand, within HER17_RS17190 at 3870.681 kb on - strand, within HER17_RS17190 at 3870.681 kb on - strand, within HER17_RS17190 at 3870.854 kb on + strand, within HER17_RS17190 at 3870.854 kb on + strand, within HER17_RS17190 at 3871.074 kb on + strand, within HER17_RS17190 at 3871.075 kb on - strand, within HER17_RS17190 at 3871.075 kb on - strand, within HER17_RS17190 at 3871.075 kb on - strand, within HER17_RS17190 at 3871.075 kb on - strand, within HER17_RS17190 at 3871.075 kb on - strand, within HER17_RS17190 at 3871.116 kb on + strand, within HER17_RS17190 at 3871.119 kb on - strand, within HER17_RS17190 at 3871.119 kb on - strand, within HER17_RS17190 at 3871.119 kb on - strand, within HER17_RS17190 at 3871.119 kb on - strand, within HER17_RS17190 at 3871.240 kb on + strand, within HER17_RS17190 at 3871.240 kb on + strand, within HER17_RS17190 at 3871.240 kb on + strand, within HER17_RS17190 at 3871.241 kb on - strand, within HER17_RS17190 at 3871.241 kb on - strand, within HER17_RS17190 at 3871.565 kb on - strand, within HER17_RS17195 at 3871.565 kb on - strand, within HER17_RS17195 at 3871.596 kb on + strand, within HER17_RS17195 at 3871.796 kb on - strand, within HER17_RS17195 at 3871.796 kb on - strand, within HER17_RS17195 at 3871.821 kb on - strand, within HER17_RS17195 at 3871.821 kb on - strand, within HER17_RS17195 at 3871.867 kb on + strand, within HER17_RS17195 at 3871.873 kb on + strand, within HER17_RS17195 at 3871.873 kb on + strand, within HER17_RS17195 at 3871.873 kb on + strand, within HER17_RS17195 at 3871.873 kb on + strand, within HER17_RS17195 at 3871.873 kb on + strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.874 kb on - strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.875 kb on + strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.876 kb on - strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.939 kb on + strand, within HER17_RS17195 at 3871.940 kb on - strand, within HER17_RS17195 at 3871.940 kb on - strand, within HER17_RS17195 at 3871.940 kb on - strand, within HER17_RS17195 at 3871.940 kb on - strand, within HER17_RS17195 at 3872.031 kb on + strand, within HER17_RS17195 at 3872.031 kb on + strand, within HER17_RS17195 at 3872.032 kb on - strand, within HER17_RS17195 at 3872.032 kb on - strand, within HER17_RS17195 at 3872.109 kb on + strand at 3872.109 kb on + strand at 3872.135 kb on + strand at 3872.135 kb on + strand at 3872.135 kb on + strand at 3872.136 kb on - strand at 3872.136 kb on - strand at 3872.136 kb on - strand at 3872.153 kb on + strand at 3872.159 kb on - strand at 3872.165 kb on - strand at 3872.188 kb on + strand at 3872.188 kb on + strand at 3872.188 kb on + strand at 3872.189 kb on - strand at 3872.193 kb on + strand at 3872.193 kb on + strand at 3872.193 kb on + strand at 3872.193 kb on + strand at 3872.194 kb on - strand at 3872.194 kb on - strand at 3872.194 kb on - strand at 3872.197 kb on + strand at 3872.198 kb on - strand at 3872.237 kb on + strand at 3872.237 kb on + strand at 3872.237 kb on + strand at 3872.237 kb on + strand at 3872.237 kb on + strand at 3872.237 kb on + strand at 3872.238 kb on - strand at 3872.238 kb on - strand at 3872.238 kb on - strand at 3872.242 kb on + strand at 3872.242 kb on + strand at 3872.243 kb on - strand at 3872.243 kb on - strand at 3872.243 kb on - strand at 3872.243 kb on - strand at 3872.243 kb on - strand at 3872.246 kb on + strand at 3872.246 kb on + strand at 3872.247 kb on - strand at 3872.247 kb on - strand at 3872.247 kb on - strand at 3872.247 kb on - strand at 3872.247 kb on - strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.248 kb on + strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.249 kb on - strand at 3872.257 kb on + strand at 3872.258 kb on - strand at 3872.258 kb on - strand at 3872.258 kb on - strand at 3872.278 kb on + strand at 3872.278 kb on + strand at 3872.294 kb on + strand at 3872.299 kb on - strand at 3872.299 kb on - strand at 3872.299 kb on - strand at 3872.357 kb on + strand at 3872.357 kb on + strand at 3872.370 kb on + strand at 3872.374 kb on + strand at 3872.375 kb on - strand at 3872.375 kb on - strand at 3872.397 kb on + strand at 3872.397 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glucose+arginine remove 3,869,197 + HER17_RS17180 0.89 -1.7 3,869,197 + HER17_RS17180 0.89 -0.9 3,869,198 - HER17_RS17180 0.89 -1.3 3,869,198 - HER17_RS17180 0.89 -0.7 3,869,202 + HER17_RS17180 0.89 -2.3 3,869,202 + HER17_RS17180 0.89 -2.6 3,869,202 + HER17_RS17180 0.89 -1.3 3,869,288 + -0.3 3,869,289 - -2.3 3,869,346 + -0.6 3,869,369 - +0.5 3,869,401 + +0.6 3,869,405 + -0.5 3,869,406 - +0.8 3,869,406 - -1.3 3,869,499 + -1.1 3,869,499 + +1.0 3,869,504 + -0.3 3,869,504 + +0.1 3,869,507 - -0.0 3,869,507 - -1.7 3,869,507 - +2.7 3,869,520 - -2.7 3,869,520 - -0.6 3,869,522 + -0.7 3,869,529 + -0.9 3,869,529 + -2.8 3,869,530 - +0.8 3,870,006 - +0.3 3,870,060 + -1.6 3,870,061 - +1.0 3,870,061 - +0.9 3,870,072 - +0.8 3,870,072 - -1.8 3,870,074 + +0.4 3,870,075 - +1.0 3,870,075 - +0.5 3,870,154 - +0.3 3,870,186 - -0.9 3,870,186 - -0.2 3,870,238 + -1.2 3,870,288 - HER17_RS17190 0.12 +0.1 3,870,299 + HER17_RS17190 0.13 -3.0 3,870,299 + HER17_RS17190 0.13 +0.2 3,870,403 - HER17_RS17190 0.21 +0.3 3,870,403 - HER17_RS17190 0.21 +0.2 3,870,524 - HER17_RS17190 0.30 +0.2 3,870,572 - HER17_RS17190 0.33 -2.2 3,870,585 - HER17_RS17190 0.34 +0.1 3,870,632 + HER17_RS17190 0.38 +0.8 3,870,632 + HER17_RS17190 0.38 +0.5 3,870,632 + HER17_RS17190 0.38 +0.5 3,870,633 - HER17_RS17190 0.38 +1.5 3,870,678 + HER17_RS17190 0.41 -1.3 3,870,678 + HER17_RS17190 0.41 +0.7 3,870,678 + HER17_RS17190 0.41 -0.2 3,870,678 + HER17_RS17190 0.41 +1.7 3,870,678 + HER17_RS17190 0.41 -2.0 3,870,678 + HER17_RS17190 0.41 -1.6 3,870,679 - HER17_RS17190 0.41 -1.4 3,870,679 - HER17_RS17190 0.41 +0.6 3,870,679 - HER17_RS17190 0.41 -0.5 3,870,680 + HER17_RS17190 0.41 -1.5 3,870,680 + HER17_RS17190 0.41 -0.1 3,870,680 + HER17_RS17190 0.41 -0.0 3,870,680 + HER17_RS17190 0.41 +0.6 3,870,681 - HER17_RS17190 0.41 -1.8 3,870,681 - HER17_RS17190 0.41 -0.5 3,870,681 - HER17_RS17190 0.41 +1.5 3,870,681 - HER17_RS17190 0.41 -0.5 3,870,854 + HER17_RS17190 0.54 -0.8 3,870,854 + HER17_RS17190 0.54 -2.7 3,871,074 + HER17_RS17190 0.70 -0.7 3,871,075 - HER17_RS17190 0.70 +0.8 3,871,075 - HER17_RS17190 0.70 +1.3 3,871,075 - HER17_RS17190 0.70 +0.8 3,871,075 - HER17_RS17190 0.70 -0.9 3,871,075 - HER17_RS17190 0.70 +0.4 3,871,116 + HER17_RS17190 0.73 -0.3 3,871,119 - HER17_RS17190 0.73 +0.8 3,871,119 - HER17_RS17190 0.73 -0.3 3,871,119 - HER17_RS17190 0.73 +0.2 3,871,119 - HER17_RS17190 0.73 +0.1 3,871,240 + HER17_RS17190 0.82 +0.9 3,871,240 + HER17_RS17190 0.82 -0.6 3,871,240 + HER17_RS17190 0.82 -0.3 3,871,241 - HER17_RS17190 0.82 +0.4 3,871,241 - HER17_RS17190 0.82 -0.5 3,871,565 - HER17_RS17195 0.12 +0.9 3,871,565 - HER17_RS17195 0.12 +1.3 3,871,596 + HER17_RS17195 0.16 -2.8 3,871,796 - HER17_RS17195 0.46 +0.5 3,871,796 - HER17_RS17195 0.46 +0.1 3,871,821 - HER17_RS17195 0.49 +1.0 3,871,821 - HER17_RS17195 0.49 -0.1 3,871,867 + HER17_RS17195 0.56 -1.9 3,871,873 + HER17_RS17195 0.57 +0.2 3,871,873 + HER17_RS17195 0.57 +1.7 3,871,873 + HER17_RS17195 0.57 +0.2 3,871,873 + HER17_RS17195 0.57 +0.9 3,871,873 + HER17_RS17195 0.57 -0.6 3,871,874 - HER17_RS17195 0.57 +0.1 3,871,874 - HER17_RS17195 0.57 -0.2 3,871,874 - HER17_RS17195 0.57 -0.0 3,871,874 - HER17_RS17195 0.57 -1.6 3,871,874 - HER17_RS17195 0.57 +1.0 3,871,874 - HER17_RS17195 0.57 +1.2 3,871,875 + HER17_RS17195 0.57 +0.4 3,871,875 + HER17_RS17195 0.57 -0.8 3,871,875 + HER17_RS17195 0.57 -1.5 3,871,875 + HER17_RS17195 0.57 -0.0 3,871,875 + HER17_RS17195 0.57 -1.7 3,871,875 + HER17_RS17195 0.57 -2.3 3,871,876 - HER17_RS17195 0.58 -1.7 3,871,876 - HER17_RS17195 0.58 +0.5 3,871,876 - HER17_RS17195 0.58 -0.4 3,871,876 - HER17_RS17195 0.58 +0.0 3,871,876 - HER17_RS17195 0.58 -0.8 3,871,876 - HER17_RS17195 0.58 +0.4 3,871,876 - HER17_RS17195 0.58 +0.8 3,871,939 + HER17_RS17195 0.67 -0.7 3,871,939 + HER17_RS17195 0.67 +0.7 3,871,939 + HER17_RS17195 0.67 -1.5 3,871,939 + HER17_RS17195 0.67 +0.6 3,871,939 + HER17_RS17195 0.67 +0.1 3,871,939 + HER17_RS17195 0.67 +0.6 3,871,940 - HER17_RS17195 0.67 -0.6 3,871,940 - HER17_RS17195 0.67 +0.8 3,871,940 - HER17_RS17195 0.67 -1.8 3,871,940 - HER17_RS17195 0.67 +1.0 3,872,031 + HER17_RS17195 0.80 +0.8 3,872,031 + HER17_RS17195 0.80 -1.8 3,872,032 - HER17_RS17195 0.80 -1.0 3,872,032 - HER17_RS17195 0.80 -1.4 3,872,109 + -1.0 3,872,109 + +0.2 3,872,135 + -2.1 3,872,135 + -0.9 3,872,135 + +0.2 3,872,136 - +0.5 3,872,136 - -0.1 3,872,136 - -1.0 3,872,153 + -1.8 3,872,159 - +0.2 3,872,165 - +0.1 3,872,188 + -1.4 3,872,188 + -3.6 3,872,188 + -0.0 3,872,189 - +0.6 3,872,193 + +0.8 3,872,193 + -0.3 3,872,193 + -1.8 3,872,193 + +0.4 3,872,194 - -0.5 3,872,194 - +0.5 3,872,194 - +0.6 3,872,197 + +1.4 3,872,198 - +0.2 3,872,237 + -0.4 3,872,237 + -0.9 3,872,237 + -1.5 3,872,237 + +0.9 3,872,237 + -0.5 3,872,237 + +0.4 3,872,238 - +0.7 3,872,238 - +1.3 3,872,238 - -0.5 3,872,242 + +0.7 3,872,242 + +0.1 3,872,243 - +0.3 3,872,243 - +0.9 3,872,243 - +1.6 3,872,243 - -1.8 3,872,243 - -0.2 3,872,246 + +2.8 3,872,246 + +0.7 3,872,247 - -0.2 3,872,247 - -2.6 3,872,247 - +0.6 3,872,247 - +0.4 3,872,247 - +0.1 3,872,248 + +0.3 3,872,248 + -1.7 3,872,248 + -1.0 3,872,248 + -1.4 3,872,248 + -0.8 3,872,248 + -1.0 3,872,248 + +0.3 3,872,248 + +1.0 3,872,248 + +1.5 3,872,248 + +0.7 3,872,248 + -0.6 3,872,248 + +0.4 3,872,248 + +0.4 3,872,248 + -1.2 3,872,248 + +1.0 3,872,248 + +1.1 3,872,248 + -0.5 3,872,249 - -1.1 3,872,249 - +0.3 3,872,249 - +0.3 3,872,249 - +0.8 3,872,249 - -0.8 3,872,249 - -0.2 3,872,249 - -1.7 3,872,249 - +0.4 3,872,249 - +0.3 3,872,249 - -1.1 3,872,249 - -1.4 3,872,249 - +0.7 3,872,249 - +0.5 3,872,249 - +0.3 3,872,249 - +0.5 3,872,249 - -1.0 3,872,249 - -0.1 3,872,249 - +0.6 3,872,257 + +0.4 3,872,258 - -0.7 3,872,258 - -1.1 3,872,258 - +0.5 3,872,278 + -1.1 3,872,278 + -0.1 3,872,294 + +0.1 3,872,299 - +0.5 3,872,299 - +0.9 3,872,299 - -1.5 3,872,357 + -0.7 3,872,357 + -3.1 3,872,370 + -0.1 3,872,374 + -1.3 3,872,375 - -3.0 3,872,375 - -2.5 3,872,397 + +0.5 3,872,397 + +0.5
Or see this region's nucleotide sequence