Experiment: M9+glucose+arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS02190 and HER17_RS02195 are separated by 40 nucleotides HER17_RS02195 and HER17_RS02200 are separated by 207 nucleotides HER17_RS02200 and HER17_RS02205 are separated by 27 nucleotides HER17_RS02205 and HER17_RS02210 are separated by 91 nucleotides
HER17_RS02190: HER17_RS02190 - DNA-directed RNA polymerase subunit alpha, at 488,891 to 489,880
_RS02190
HER17_RS02195: HER17_RS02195 - 50S ribosomal protein L17, at 489,921 to 490,313
_RS02195
HER17_RS02200: HER17_RS02200 - alternative ribosome-rescue factor A, at 490,521 to 490,736
_RS02200
HER17_RS02205: HER17_RS02205 - large-conductance mechanosensitive channel protein MscL, at 490,764 to 491,177
_RS02205
HER17_RS02210: HER17_RS02210 - Trk system potassium transporter TrkA, at 491,269 to 492,645
_RS02210
Position (kb) 490
491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 490.366 kb on - strand at 490.382 kb on + strand at 490.382 kb on + strand at 490.382 kb on + strand at 490.403 kb on + strand at 490.403 kb on + strand at 490.403 kb on + strand at 490.404 kb on - strand at 490.404 kb on - strand at 490.404 kb on - strand at 490.404 kb on - strand at 490.409 kb on + strand at 490.410 kb on - strand at 490.410 kb on - strand at 490.446 kb on + strand at 490.446 kb on + strand at 490.446 kb on + strand at 490.446 kb on + strand at 490.447 kb on - strand at 490.447 kb on - strand at 490.447 kb on - strand at 490.451 kb on - strand at 490.451 kb on - strand at 490.465 kb on + strand at 490.465 kb on + strand at 490.466 kb on - strand at 490.467 kb on + strand at 490.467 kb on + strand at 490.467 kb on + strand at 490.467 kb on + strand at 490.467 kb on + strand at 490.468 kb on - strand at 490.468 kb on - strand at 490.468 kb on - strand at 490.482 kb on - strand at 490.490 kb on - strand at 490.490 kb on - strand at 490.490 kb on - strand at 490.490 kb on - strand at 490.490 kb on - strand at 490.493 kb on + strand at 490.494 kb on - strand at 490.511 kb on - strand at 490.530 kb on + strand at 490.530 kb on + strand at 490.530 kb on + strand at 490.530 kb on + strand at 490.531 kb on - strand at 490.531 kb on - strand at 490.580 kb on - strand, within HER17_RS02200 at 490.580 kb on - strand, within HER17_RS02200 at 490.580 kb on - strand, within HER17_RS02200 at 490.580 kb on - strand, within HER17_RS02200 at 490.636 kb on - strand, within HER17_RS02200 at 490.667 kb on + strand, within HER17_RS02200 at 490.720 kb on + strand at 490.739 kb on + strand at 490.751 kb on - strand at 490.751 kb on - strand at 490.809 kb on + strand, within HER17_RS02205 at 490.809 kb on + strand, within HER17_RS02205 at 490.809 kb on + strand, within HER17_RS02205 at 490.812 kb on + strand, within HER17_RS02205 at 490.812 kb on + strand, within HER17_RS02205 at 490.813 kb on - strand, within HER17_RS02205 at 490.813 kb on - strand, within HER17_RS02205 at 490.853 kb on + strand, within HER17_RS02205 at 490.854 kb on - strand, within HER17_RS02205 at 490.877 kb on + strand, within HER17_RS02205 at 490.883 kb on + strand, within HER17_RS02205 at 490.884 kb on - strand, within HER17_RS02205 at 490.922 kb on + strand, within HER17_RS02205 at 490.923 kb on - strand, within HER17_RS02205 at 490.923 kb on - strand, within HER17_RS02205 at 490.955 kb on - strand, within HER17_RS02205 at 490.955 kb on - strand, within HER17_RS02205 at 491.054 kb on + strand, within HER17_RS02205 at 491.057 kb on + strand, within HER17_RS02205 at 491.057 kb on + strand, within HER17_RS02205 at 491.057 kb on + strand, within HER17_RS02205 at 491.058 kb on - strand, within HER17_RS02205 at 491.060 kb on + strand, within HER17_RS02205 at 491.061 kb on - strand, within HER17_RS02205 at 491.105 kb on + strand, within HER17_RS02205 at 491.166 kb on - strand at 491.240 kb on + strand at 491.253 kb on + strand at 491.253 kb on + strand at 491.253 kb on + strand at 491.253 kb on + strand at 491.253 kb on + strand at 491.254 kb on - strand at 491.254 kb on - strand at 491.270 kb on + strand at 491.270 kb on + strand at 491.419 kb on - strand, within HER17_RS02210 at 491.419 kb on - strand, within HER17_RS02210 at 491.440 kb on - strand, within HER17_RS02210 at 491.527 kb on + strand, within HER17_RS02210 at 491.543 kb on - strand, within HER17_RS02210 at 491.701 kb on - strand, within HER17_RS02210
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glucose+arginine remove 490,366 - -0.2 490,382 + +1.2 490,382 + -0.3 490,382 + +1.1 490,403 + +1.1 490,403 + +1.0 490,403 + +2.2 490,404 - -0.6 490,404 - -0.5 490,404 - +1.7 490,404 - -0.4 490,409 + +1.1 490,410 - +2.0 490,410 - -0.7 490,446 + -2.4 490,446 + -0.5 490,446 + +0.3 490,446 + +0.4 490,447 - -2.5 490,447 - +0.4 490,447 - +0.9 490,451 - -1.8 490,451 - -0.1 490,465 + +0.9 490,465 + -0.9 490,466 - +0.8 490,467 + -0.8 490,467 + -2.9 490,467 + +1.5 490,467 + +0.5 490,467 + -0.9 490,468 - +1.1 490,468 - -1.1 490,468 - -0.4 490,482 - -0.8 490,490 - -2.6 490,490 - -3.6 490,490 - -0.1 490,490 - +0.3 490,490 - -0.4 490,493 + +1.8 490,494 - +0.4 490,511 - -1.4 490,530 + +1.0 490,530 + +2.0 490,530 + +1.5 490,530 + -1.2 490,531 - +0.3 490,531 - +0.6 490,580 - HER17_RS02200 0.27 +0.8 490,580 - HER17_RS02200 0.27 -0.4 490,580 - HER17_RS02200 0.27 -0.7 490,580 - HER17_RS02200 0.27 +0.8 490,636 - HER17_RS02200 0.53 -0.1 490,667 + HER17_RS02200 0.68 -1.8 490,720 + +1.4 490,739 + +1.6 490,751 - +0.6 490,751 - +1.8 490,809 + HER17_RS02205 0.11 +0.8 490,809 + HER17_RS02205 0.11 +0.8 490,809 + HER17_RS02205 0.11 +0.6 490,812 + HER17_RS02205 0.12 +1.1 490,812 + HER17_RS02205 0.12 -0.2 490,813 - HER17_RS02205 0.12 -0.7 490,813 - HER17_RS02205 0.12 +0.6 490,853 + HER17_RS02205 0.21 -0.2 490,854 - HER17_RS02205 0.22 +0.9 490,877 + HER17_RS02205 0.27 -1.2 490,883 + HER17_RS02205 0.29 -1.7 490,884 - HER17_RS02205 0.29 +1.1 490,922 + HER17_RS02205 0.38 -0.5 490,923 - HER17_RS02205 0.38 +0.2 490,923 - HER17_RS02205 0.38 +0.2 490,955 - HER17_RS02205 0.46 +0.3 490,955 - HER17_RS02205 0.46 +0.4 491,054 + HER17_RS02205 0.70 +0.2 491,057 + HER17_RS02205 0.71 -0.9 491,057 + HER17_RS02205 0.71 -0.7 491,057 + HER17_RS02205 0.71 +0.5 491,058 - HER17_RS02205 0.71 +0.5 491,060 + HER17_RS02205 0.71 -1.2 491,061 - HER17_RS02205 0.72 +1.6 491,105 + HER17_RS02205 0.82 +1.3 491,166 - +0.1 491,240 + -0.5 491,253 + -1.2 491,253 + -1.5 491,253 + +1.4 491,253 + +0.9 491,253 + +0.7 491,254 - -0.5 491,254 - +1.1 491,270 + +1.5 491,270 + -0.0 491,419 - HER17_RS02210 0.11 +0.1 491,419 - HER17_RS02210 0.11 -1.2 491,440 - HER17_RS02210 0.12 +0.4 491,527 + HER17_RS02210 0.19 -0.9 491,543 - HER17_RS02210 0.20 +0.1 491,701 - HER17_RS02210 0.31 -2.2
Or see this region's nucleotide sequence