Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS05705 and HER17_RS05715 are separated by 325 nucleotides HER17_RS05715 and HER17_RS05720 are separated by 7 nucleotides HER17_RS05720 and HER17_RS05725 are separated by 55 nucleotides HER17_RS05725 and HER17_RS05730 are separated by 53 nucleotides HER17_RS05730 and HER17_RS05735 are separated by 291 nucleotides
HER17_RS05705: HER17_RS05705 - NADH:flavin oxidoreductase, at 1,258,703 to 1,259,809
_RS05705
HER17_RS05715: HER17_RS05715 - tRNA-Lys, at 1,260,135 to 1,260,210
_RS05715
HER17_RS05720: HER17_RS05720 - tRNA-Val, at 1,260,218 to 1,260,293
_RS05720
HER17_RS05725: HER17_RS05725 - tRNA-Val, at 1,260,349 to 1,260,424
_RS05725
HER17_RS05730: HER17_RS05730 - tRNA-Val, at 1,260,478 to 1,260,553
_RS05730
HER17_RS05735: HER17_RS05735 - glutamate--tRNA ligase, at 1,260,845 to 1,262,260
_RS05735
Position (kb)
1260
1261 Strain fitness (log2 ratio)
-2
-1
0
1 at 1259.390 kb on + strand, within HER17_RS05705 at 1259.391 kb on - strand, within HER17_RS05705 at 1259.464 kb on + strand, within HER17_RS05705 at 1259.663 kb on + strand, within HER17_RS05705 at 1259.664 kb on - strand, within HER17_RS05705 at 1259.765 kb on - strand at 1259.765 kb on - strand at 1259.810 kb on + strand at 1259.810 kb on + strand at 1259.811 kb on - strand at 1259.814 kb on + strand at 1259.814 kb on + strand at 1259.823 kb on + strand at 1259.824 kb on - strand at 1259.832 kb on - strand at 1259.842 kb on + strand at 1259.843 kb on - strand at 1259.843 kb on - strand at 1259.855 kb on - strand at 1259.857 kb on + strand at 1259.857 kb on + strand at 1259.867 kb on - strand at 1259.871 kb on + strand at 1259.912 kb on + strand at 1259.912 kb on + strand at 1259.913 kb on - strand at 1259.923 kb on + strand at 1259.933 kb on - strand at 1260.070 kb on + strand at 1260.086 kb on + strand at 1260.086 kb on + strand at 1260.087 kb on - strand at 1260.087 kb on - strand at 1260.087 kb on - strand at 1260.090 kb on + strand at 1260.260 kb on - strand, within HER17_RS05720 at 1260.305 kb on - strand at 1260.311 kb on - strand at 1260.446 kb on - strand at 1260.636 kb on + strand at 1260.651 kb on - strand at 1260.655 kb on - strand at 1260.655 kb on - strand at 1260.694 kb on + strand at 1260.694 kb on + strand at 1260.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 1,259,390 + HER17_RS05705 0.62 -1.7 1,259,391 - HER17_RS05705 0.62 -1.1 1,259,464 + HER17_RS05705 0.69 -1.3 1,259,663 + HER17_RS05705 0.87 -0.3 1,259,664 - HER17_RS05705 0.87 -0.6 1,259,765 - +0.8 1,259,765 - -0.2 1,259,810 + -0.3 1,259,810 + -0.5 1,259,811 - +0.3 1,259,814 + -0.2 1,259,814 + +0.1 1,259,823 + +0.1 1,259,824 - -0.7 1,259,832 - -1.2 1,259,842 + +0.8 1,259,843 - +0.6 1,259,843 - +0.8 1,259,855 - -0.1 1,259,857 + +0.9 1,259,857 + -0.5 1,259,867 - -2.0 1,259,871 + +0.0 1,259,912 + +1.2 1,259,912 + -0.4 1,259,913 - -0.5 1,259,923 + +0.8 1,259,933 - +0.8 1,260,070 + -0.0 1,260,086 + +0.5 1,260,086 + +0.1 1,260,087 - +0.2 1,260,087 - -1.6 1,260,087 - +0.7 1,260,090 + -0.9 1,260,260 - HER17_RS05720 0.55 +0.2 1,260,305 - +1.5 1,260,311 - -0.3 1,260,446 - +0.8 1,260,636 + +0.3 1,260,651 - +0.5 1,260,655 - -0.9 1,260,655 - -0.2 1,260,694 + +0.0 1,260,694 + -0.2 1,260,737 - -0.8
Or see this region's nucleotide sequence