Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15220 and HER17_RS15225 overlap by 4 nucleotides HER17_RS15225 and HER17_RS15230 overlap by 34 nucleotides HER17_RS15230 and HER17_RS15235 are separated by 194 nucleotides HER17_RS15235 and HER17_RS15240 are separated by 1 nucleotides
HER17_RS15220: HER17_RS15220 - LPS assembly lipoprotein LptE, at 3,415,804 to 3,416,358
_RS15220
HER17_RS15225: HER17_RS15225 - DNA polymerase III subunit delta, at 3,416,355 to 3,417,380
_RS15225
HER17_RS15230: HER17_RS15230 - nicotinate-nucleotide adenylyltransferase, at 3,417,347 to 3,418,036
_RS15230
HER17_RS15235: HER17_RS15235 - ribosome silencing factor, at 3,418,231 to 3,418,548
_RS15235
HER17_RS15240: HER17_RS15240 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 3,418,550 to 3,419,020
_RS15240
Position (kb)
3417
3418
3419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3416.415 kb on + strand at 3417.382 kb on + strand at 3418.028 kb on + strand at 3418.035 kb on - strand at 3418.069 kb on + strand at 3418.069 kb on + strand at 3418.070 kb on - strand at 3418.070 kb on - strand at 3418.071 kb on + strand at 3418.094 kb on + strand at 3418.094 kb on + strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.266 kb on + strand, within HER17_RS15235 at 3418.266 kb on + strand, within HER17_RS15235 at 3418.266 kb on + strand, within HER17_RS15235 at 3418.280 kb on + strand, within HER17_RS15235 at 3418.280 kb on + strand, within HER17_RS15235 at 3418.305 kb on + strand, within HER17_RS15235 at 3418.336 kb on + strand, within HER17_RS15235 at 3418.336 kb on + strand, within HER17_RS15235 at 3418.344 kb on + strand, within HER17_RS15235 at 3418.362 kb on + strand, within HER17_RS15235 at 3418.380 kb on + strand, within HER17_RS15235 at 3418.475 kb on + strand, within HER17_RS15235 at 3418.522 kb on + strand at 3418.529 kb on + strand at 3418.549 kb on + strand at 3418.762 kb on + strand, within HER17_RS15240 at 3418.815 kb on + strand, within HER17_RS15240 at 3419.026 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 3,416,415 + +0.1 3,417,382 + +1.5 3,418,028 + -0.1 3,418,035 - +0.6 3,418,069 + -0.6 3,418,069 + -0.5 3,418,070 - -0.7 3,418,070 - +0.4 3,418,071 + -0.4 3,418,094 + +0.8 3,418,094 + -0.1 3,418,097 - -0.7 3,418,097 - -0.4 3,418,097 - +0.1 3,418,097 - -1.9 3,418,266 + HER17_RS15235 0.11 -0.2 3,418,266 + HER17_RS15235 0.11 -0.0 3,418,266 + HER17_RS15235 0.11 -0.0 3,418,280 + HER17_RS15235 0.15 +0.3 3,418,280 + HER17_RS15235 0.15 +0.3 3,418,305 + HER17_RS15235 0.23 +0.6 3,418,336 + HER17_RS15235 0.33 +0.4 3,418,336 + HER17_RS15235 0.33 +1.1 3,418,344 + HER17_RS15235 0.36 +1.1 3,418,362 + HER17_RS15235 0.41 +0.4 3,418,380 + HER17_RS15235 0.47 +0.8 3,418,475 + HER17_RS15235 0.77 +1.8 3,418,522 + +0.5 3,418,529 + +0.7 3,418,549 + +0.0 3,418,762 + HER17_RS15240 0.45 +0.0 3,418,815 + HER17_RS15240 0.56 -0.1 3,419,026 + -1.9
Or see this region's nucleotide sequence