Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS17140 and HER17_RS17145 are separated by 103 nucleotides HER17_RS17145 and HER17_RS17150 are separated by 485 nucleotides HER17_RS17150 and HER17_RS17155 are separated by 135 nucleotides
HER17_RS17140: HER17_RS17140 - SAM-dependent methyltransferase TehB, at 3,859,797 to 3,860,657
_RS17140
HER17_RS17145: HER17_RS17145 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 3,860,761 to 3,861,414
_RS17145
HER17_RS17150: HER17_RS17150 - ubiquinone biosynthesis accessory factor UbiK, at 3,861,900 to 3,862,190
_RS17150
HER17_RS17155: HER17_RS17155 - L-methionine/branched-chain amino acid transporter, at 3,862,326 to 3,863,579
_RS17155
Position (kb)
3860
3861
3862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3859.815 kb on + strand at 3859.815 kb on + strand at 3859.818 kb on - strand at 3859.818 kb on - strand at 3859.955 kb on + strand, within HER17_RS17140 at 3860.089 kb on - strand, within HER17_RS17140 at 3860.094 kb on - strand, within HER17_RS17140 at 3860.094 kb on - strand, within HER17_RS17140 at 3860.174 kb on - strand, within HER17_RS17140 at 3860.203 kb on - strand, within HER17_RS17140 at 3860.203 kb on - strand, within HER17_RS17140 at 3860.270 kb on + strand, within HER17_RS17140 at 3860.271 kb on - strand, within HER17_RS17140 at 3860.349 kb on + strand, within HER17_RS17140 at 3860.420 kb on + strand, within HER17_RS17140 at 3860.420 kb on + strand, within HER17_RS17140 at 3860.448 kb on + strand, within HER17_RS17140 at 3860.451 kb on - strand, within HER17_RS17140 at 3860.517 kb on - strand, within HER17_RS17140 at 3860.538 kb on + strand, within HER17_RS17140 at 3860.538 kb on + strand, within HER17_RS17140 at 3860.538 kb on + strand, within HER17_RS17140 at 3860.539 kb on - strand, within HER17_RS17140 at 3860.539 kb on - strand, within HER17_RS17140 at 3860.542 kb on - strand, within HER17_RS17140 at 3860.565 kb on + strand, within HER17_RS17140 at 3860.565 kb on + strand, within HER17_RS17140 at 3860.566 kb on - strand, within HER17_RS17140 at 3860.566 kb on - strand, within HER17_RS17140 at 3860.568 kb on - strand, within HER17_RS17140 at 3860.587 kb on + strand at 3860.588 kb on - strand at 3860.738 kb on + strand at 3860.746 kb on + strand at 3860.748 kb on + strand at 3860.748 kb on + strand at 3860.749 kb on - strand at 3860.749 kb on - strand at 3861.497 kb on + strand at 3861.667 kb on - strand at 3861.731 kb on - strand at 3861.740 kb on - strand at 3861.773 kb on + strand at 3861.800 kb on + strand at 3861.806 kb on + strand at 3861.815 kb on + strand at 3861.815 kb on + strand at 3861.815 kb on + strand at 3861.815 kb on + strand at 3861.815 kb on + strand at 3861.816 kb on - strand at 3861.816 kb on - strand at 3861.816 kb on - strand at 3861.816 kb on - strand at 3861.819 kb on + strand at 3861.819 kb on + strand at 3861.820 kb on - strand at 3861.820 kb on - strand at 3861.820 kb on - strand at 3861.845 kb on - strand at 3861.845 kb on - strand at 3861.846 kb on + strand at 3861.847 kb on - strand at 3861.864 kb on + strand at 3861.864 kb on + strand at 3861.864 kb on + strand at 3861.867 kb on + strand at 3861.867 kb on + strand at 3861.868 kb on - strand at 3861.868 kb on - strand at 3861.870 kb on + strand at 3861.871 kb on - strand at 3861.871 kb on - strand at 3861.871 kb on - strand at 3861.875 kb on + strand at 3861.876 kb on - strand at 3861.884 kb on + strand at 3862.115 kb on + strand, within HER17_RS17150 at 3862.116 kb on - strand, within HER17_RS17150 at 3862.116 kb on - strand, within HER17_RS17150 at 3862.188 kb on + strand at 3862.188 kb on + strand at 3862.189 kb on - strand at 3862.228 kb on - strand at 3862.338 kb on + strand at 3862.338 kb on + strand at 3862.338 kb on + strand at 3862.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 3,859,815 + -0.2 3,859,815 + +1.0 3,859,818 - +0.0 3,859,818 - +0.2 3,859,955 + HER17_RS17140 0.18 +0.2 3,860,089 - HER17_RS17140 0.34 -0.9 3,860,094 - HER17_RS17140 0.34 +0.4 3,860,094 - HER17_RS17140 0.34 -0.5 3,860,174 - HER17_RS17140 0.44 -0.4 3,860,203 - HER17_RS17140 0.47 +0.1 3,860,203 - HER17_RS17140 0.47 +1.7 3,860,270 + HER17_RS17140 0.55 +0.2 3,860,271 - HER17_RS17140 0.55 -0.9 3,860,349 + HER17_RS17140 0.64 -0.2 3,860,420 + HER17_RS17140 0.72 +0.2 3,860,420 + HER17_RS17140 0.72 +0.1 3,860,448 + HER17_RS17140 0.76 -0.4 3,860,451 - HER17_RS17140 0.76 +0.5 3,860,517 - HER17_RS17140 0.84 -0.0 3,860,538 + HER17_RS17140 0.86 +0.2 3,860,538 + HER17_RS17140 0.86 -0.8 3,860,538 + HER17_RS17140 0.86 +1.2 3,860,539 - HER17_RS17140 0.86 +0.3 3,860,539 - HER17_RS17140 0.86 +0.0 3,860,542 - HER17_RS17140 0.87 -0.3 3,860,565 + HER17_RS17140 0.89 +0.7 3,860,565 + HER17_RS17140 0.89 +0.3 3,860,566 - HER17_RS17140 0.89 +0.7 3,860,566 - HER17_RS17140 0.89 +0.5 3,860,568 - HER17_RS17140 0.90 -0.1 3,860,587 + -0.2 3,860,588 - -1.4 3,860,738 + -0.4 3,860,746 + +0.3 3,860,748 + +0.2 3,860,748 + +0.9 3,860,749 - -0.3 3,860,749 - -0.7 3,861,497 + -2.0 3,861,667 - -0.4 3,861,731 - -1.0 3,861,740 - -0.6 3,861,773 + -0.1 3,861,800 + -0.3 3,861,806 + +0.1 3,861,815 + +0.9 3,861,815 + -0.0 3,861,815 + +0.2 3,861,815 + -0.2 3,861,815 + -0.6 3,861,816 - +1.9 3,861,816 - +0.3 3,861,816 - -0.3 3,861,816 - +0.3 3,861,819 + +0.4 3,861,819 + -0.6 3,861,820 - +0.7 3,861,820 - -0.3 3,861,820 - -2.6 3,861,845 - -0.8 3,861,845 - -0.3 3,861,846 + +0.2 3,861,847 - +0.4 3,861,864 + -0.2 3,861,864 + -0.0 3,861,864 + -0.1 3,861,867 + -0.5 3,861,867 + +0.3 3,861,868 - +0.0 3,861,868 - -0.3 3,861,870 + +1.5 3,861,871 - +0.3 3,861,871 - -0.5 3,861,871 - -0.2 3,861,875 + +0.8 3,861,876 - +0.0 3,861,884 + +0.1 3,862,115 + HER17_RS17150 0.74 -0.1 3,862,116 - HER17_RS17150 0.74 +1.0 3,862,116 - HER17_RS17150 0.74 +0.6 3,862,188 + +0.0 3,862,188 + +0.4 3,862,189 - -0.8 3,862,228 - +0.3 3,862,338 + -2.8 3,862,338 + +0.6 3,862,338 + -0.3 3,862,339 - +0.1
Or see this region's nucleotide sequence