Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15945 and HER17_RS15950 are separated by 68 nucleotides HER17_RS15950 and HER17_RS15955 are separated by 24 nucleotides HER17_RS15955 and HER17_RS15960 are separated by 110 nucleotides
HER17_RS15945: HER17_RS15945 - thiamine-phosphate kinase, at 3,581,418 to 3,582,398
_RS15945
HER17_RS15950: HER17_RS15950 - transcription antitermination factor NusB, at 3,582,467 to 3,582,886
_RS15950
HER17_RS15955: HER17_RS15955 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,582,911 to 3,583,387
_RS15955
HER17_RS15960: HER17_RS15960 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 3,583,498 to 3,584,634
_RS15960
Position (kb)
3582
3583 Strain fitness (log2 ratio)
-1
0
1 at 3583.485 kb on - strand at 3583.485 kb on - strand at 3583.485 kb on - strand at 3583.485 kb on - strand at 3583.488 kb on + strand at 3583.489 kb on - strand at 3583.489 kb on - strand at 3583.489 kb on - strand at 3583.489 kb on - strand at 3583.489 kb on - strand at 3583.492 kb on + strand at 3583.492 kb on + strand at 3583.492 kb on + strand at 3583.493 kb on - strand at 3583.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 3,583,485 - -0.2 3,583,485 - +0.1 3,583,485 - +0.0 3,583,485 - +0.2 3,583,488 + -0.3 3,583,489 - +0.0 3,583,489 - +0.7 3,583,489 - -0.1 3,583,489 - +0.1 3,583,489 - +0.4 3,583,492 + -0.2 3,583,492 + +0.6 3,583,492 + -0.1 3,583,493 - +0.3 3,583,507 - -0.6
Or see this region's nucleotide sequence