Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS14980 and HER17_RS14985 overlap by 7 nucleotides HER17_RS14985 and HER17_RS14990 are separated by 156 nucleotides HER17_RS14990 and HER17_RS14995 overlap by 8 nucleotides
HER17_RS14980: HER17_RS14980 - biotin-dependent carboxyltransferase family protein, at 3,364,569 to 3,365,513
_RS14980
HER17_RS14985: HER17_RS14985 - 5-oxoprolinase subunit PxpB, at 3,365,507 to 3,366,163
_RS14985
HER17_RS14990: HER17_RS14990 - type 2 GTP cyclohydrolase I, at 3,366,320 to 3,367,063
_RS14990
HER17_RS14995: HER17_RS14995 - deoxyribodipyrimidine photo-lyase, at 3,367,056 to 3,368,534
_RS14995
Position (kb)
3366
3367
3368 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3365.348 kb on + strand, within HER17_RS14980 at 3365.483 kb on + strand at 3365.484 kb on - strand at 3365.484 kb on - strand at 3365.484 kb on - strand at 3365.488 kb on - strand at 3365.488 kb on - strand at 3365.488 kb on - strand at 3365.513 kb on + strand at 3365.513 kb on + strand at 3365.514 kb on - strand at 3365.514 kb on - strand at 3365.514 kb on - strand at 3365.514 kb on - strand at 3365.587 kb on + strand, within HER17_RS14985 at 3365.588 kb on - strand, within HER17_RS14985 at 3365.722 kb on + strand, within HER17_RS14985 at 3365.722 kb on + strand, within HER17_RS14985 at 3365.723 kb on - strand, within HER17_RS14985 at 3365.752 kb on - strand, within HER17_RS14985 at 3365.790 kb on + strand, within HER17_RS14985 at 3365.791 kb on - strand, within HER17_RS14985 at 3365.791 kb on - strand, within HER17_RS14985 at 3365.791 kb on - strand, within HER17_RS14985 at 3365.791 kb on - strand, within HER17_RS14985 at 3365.791 kb on - strand, within HER17_RS14985 at 3365.883 kb on + strand, within HER17_RS14985 at 3365.884 kb on - strand, within HER17_RS14985 at 3365.884 kb on - strand, within HER17_RS14985 at 3366.144 kb on + strand at 3366.145 kb on - strand at 3366.186 kb on + strand at 3366.187 kb on - strand at 3366.227 kb on - strand at 3366.227 kb on - strand at 3366.227 kb on - strand at 3366.235 kb on - strand at 3366.235 kb on - strand at 3366.253 kb on - strand at 3366.253 kb on - strand at 3366.321 kb on + strand at 3366.322 kb on - strand at 3366.322 kb on - strand at 3366.332 kb on - strand at 3366.343 kb on + strand at 3366.344 kb on - strand at 3366.344 kb on - strand at 3366.494 kb on - strand, within HER17_RS14990 at 3366.529 kb on + strand, within HER17_RS14990 at 3366.570 kb on - strand, within HER17_RS14990 at 3366.607 kb on + strand, within HER17_RS14990 at 3366.626 kb on + strand, within HER17_RS14990 at 3366.676 kb on - strand, within HER17_RS14990 at 3366.784 kb on - strand, within HER17_RS14990 at 3366.823 kb on + strand, within HER17_RS14990 at 3366.833 kb on - strand, within HER17_RS14990 at 3366.952 kb on + strand, within HER17_RS14990 at 3366.952 kb on + strand, within HER17_RS14990 at 3366.953 kb on - strand, within HER17_RS14990 at 3366.953 kb on - strand, within HER17_RS14990 at 3366.960 kb on - strand, within HER17_RS14990 at 3366.960 kb on - strand, within HER17_RS14990 at 3366.984 kb on - strand, within HER17_RS14990 at 3367.000 kb on - strand at 3367.000 kb on - strand at 3367.063 kb on + strand at 3367.063 kb on + strand at 3367.063 kb on + strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.064 kb on - strand at 3367.067 kb on + strand at 3367.068 kb on - strand at 3367.068 kb on - strand at 3367.103 kb on + strand at 3367.133 kb on + strand at 3367.174 kb on + strand at 3367.224 kb on - strand, within HER17_RS14995 at 3367.224 kb on - strand, within HER17_RS14995 at 3367.268 kb on + strand, within HER17_RS14995 at 3367.269 kb on - strand, within HER17_RS14995 at 3367.327 kb on + strand, within HER17_RS14995 at 3367.412 kb on + strand, within HER17_RS14995 at 3367.412 kb on + strand, within HER17_RS14995 at 3367.417 kb on + strand, within HER17_RS14995 at 3367.417 kb on + strand, within HER17_RS14995 at 3367.417 kb on + strand, within HER17_RS14995 at 3367.417 kb on + strand, within HER17_RS14995 at 3367.418 kb on - strand, within HER17_RS14995 at 3367.418 kb on - strand, within HER17_RS14995 at 3367.418 kb on - strand, within HER17_RS14995 at 3367.446 kb on + strand, within HER17_RS14995 at 3367.573 kb on - strand, within HER17_RS14995 at 3367.635 kb on - strand, within HER17_RS14995 at 3367.635 kb on - strand, within HER17_RS14995 at 3367.670 kb on - strand, within HER17_RS14995 at 3367.798 kb on + strand, within HER17_RS14995 at 3367.798 kb on + strand, within HER17_RS14995 at 3367.852 kb on + strand, within HER17_RS14995 at 3367.948 kb on + strand, within HER17_RS14995
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 3,365,348 + HER17_RS14980 0.82 +0.4 3,365,483 + +0.5 3,365,484 - -1.1 3,365,484 - +0.1 3,365,484 - -0.4 3,365,488 - -0.4 3,365,488 - -0.3 3,365,488 - +0.1 3,365,513 + +0.3 3,365,513 + -0.3 3,365,514 - +0.0 3,365,514 - +0.6 3,365,514 - -0.5 3,365,514 - -0.1 3,365,587 + HER17_RS14985 0.12 +0.6 3,365,588 - HER17_RS14985 0.12 +0.5 3,365,722 + HER17_RS14985 0.33 +0.6 3,365,722 + HER17_RS14985 0.33 +0.5 3,365,723 - HER17_RS14985 0.33 -0.3 3,365,752 - HER17_RS14985 0.37 +0.1 3,365,790 + HER17_RS14985 0.43 -0.1 3,365,791 - HER17_RS14985 0.43 +1.1 3,365,791 - HER17_RS14985 0.43 -1.0 3,365,791 - HER17_RS14985 0.43 +0.1 3,365,791 - HER17_RS14985 0.43 -0.9 3,365,791 - HER17_RS14985 0.43 -0.5 3,365,883 + HER17_RS14985 0.57 +0.0 3,365,884 - HER17_RS14985 0.57 -0.5 3,365,884 - HER17_RS14985 0.57 +0.5 3,366,144 + +1.0 3,366,145 - -0.2 3,366,186 + +0.6 3,366,187 - -0.3 3,366,227 - +0.3 3,366,227 - +0.4 3,366,227 - -1.3 3,366,235 - +0.6 3,366,235 - +0.3 3,366,253 - -0.4 3,366,253 - +0.0 3,366,321 + -0.6 3,366,322 - +0.1 3,366,322 - +0.3 3,366,332 - -0.1 3,366,343 + +0.8 3,366,344 - -0.1 3,366,344 - +0.0 3,366,494 - HER17_RS14990 0.23 -0.1 3,366,529 + HER17_RS14990 0.28 +0.3 3,366,570 - HER17_RS14990 0.34 +0.5 3,366,607 + HER17_RS14990 0.39 +0.3 3,366,626 + HER17_RS14990 0.41 -0.4 3,366,676 - HER17_RS14990 0.48 +1.3 3,366,784 - HER17_RS14990 0.62 +0.5 3,366,823 + HER17_RS14990 0.68 -0.9 3,366,833 - HER17_RS14990 0.69 +0.2 3,366,952 + HER17_RS14990 0.85 +0.1 3,366,952 + HER17_RS14990 0.85 -1.7 3,366,953 - HER17_RS14990 0.85 +0.6 3,366,953 - HER17_RS14990 0.85 +1.1 3,366,960 - HER17_RS14990 0.86 +0.0 3,366,960 - HER17_RS14990 0.86 +0.5 3,366,984 - HER17_RS14990 0.89 -0.7 3,367,000 - +1.8 3,367,000 - -0.2 3,367,063 + +0.5 3,367,063 + -0.2 3,367,063 + -0.6 3,367,064 - +1.3 3,367,064 - -0.0 3,367,064 - -0.0 3,367,064 - -0.1 3,367,064 - -0.7 3,367,064 - -0.1 3,367,064 - +0.3 3,367,067 + +0.6 3,367,068 - -0.4 3,367,068 - -0.4 3,367,103 + -0.1 3,367,133 + +0.3 3,367,174 + -0.2 3,367,224 - HER17_RS14995 0.11 -0.3 3,367,224 - HER17_RS14995 0.11 +0.4 3,367,268 + HER17_RS14995 0.14 -1.1 3,367,269 - HER17_RS14995 0.14 +0.3 3,367,327 + HER17_RS14995 0.18 +0.9 3,367,412 + HER17_RS14995 0.24 -2.5 3,367,412 + HER17_RS14995 0.24 -2.7 3,367,417 + HER17_RS14995 0.24 -0.1 3,367,417 + HER17_RS14995 0.24 +0.3 3,367,417 + HER17_RS14995 0.24 +0.0 3,367,417 + HER17_RS14995 0.24 +0.2 3,367,418 - HER17_RS14995 0.24 -0.5 3,367,418 - HER17_RS14995 0.24 +0.5 3,367,418 - HER17_RS14995 0.24 -0.3 3,367,446 + HER17_RS14995 0.26 -0.8 3,367,573 - HER17_RS14995 0.35 -0.5 3,367,635 - HER17_RS14995 0.39 -0.3 3,367,635 - HER17_RS14995 0.39 +0.6 3,367,670 - HER17_RS14995 0.42 +0.7 3,367,798 + HER17_RS14995 0.50 +0.7 3,367,798 + HER17_RS14995 0.50 -0.3 3,367,852 + HER17_RS14995 0.54 +0.3 3,367,948 + HER17_RS14995 0.60 +0.2
Or see this region's nucleotide sequence