Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS09965 and HER17_RS09970 are separated by 10 nucleotides HER17_RS09970 and HER17_RS09975 are separated by 6 nucleotides HER17_RS09975 and HER17_RS09980 are separated by 7 nucleotides HER17_RS09980 and HER17_RS09985 overlap by 4 nucleotides
HER17_RS09965: HER17_RS09965 - type III secretion system export apparatus subunit SctU, at 2,204,928 to 2,206,007
_RS09965
HER17_RS09970: HER17_RS09970 - type III secretion system export apparatus subunit SctT, at 2,206,018 to 2,206,821
_RS09970
HER17_RS09975: HER17_RS09975 - type III secretion system export apparatus subunit SctS, at 2,206,828 to 2,207,088
_RS09975
HER17_RS09980: HER17_RS09980 - type III secretion system export apparatus subunit SctR, at 2,207,096 to 2,207,749
_RS09980
HER17_RS09985: HER17_RS09985 - type III secretion system cytoplasmic ring protein SctQ, at 2,207,746 to 2,208,900
_RS09985
Position (kb)
2206
2207
2208 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2205.840 kb on + strand, within HER17_RS09965 at 2205.841 kb on - strand, within HER17_RS09965 at 2205.841 kb on - strand, within HER17_RS09965 at 2205.886 kb on - strand, within HER17_RS09965 at 2206.039 kb on - strand at 2206.096 kb on + strand at 2206.101 kb on - strand, within HER17_RS09970 at 2206.191 kb on + strand, within HER17_RS09970 at 2206.191 kb on + strand, within HER17_RS09970 at 2206.204 kb on + strand, within HER17_RS09970 at 2206.298 kb on + strand, within HER17_RS09970 at 2206.299 kb on - strand, within HER17_RS09970 at 2206.359 kb on - strand, within HER17_RS09970 at 2206.584 kb on + strand, within HER17_RS09970 at 2206.743 kb on + strand at 2206.776 kb on + strand at 2206.777 kb on - strand at 2206.831 kb on + strand at 2206.832 kb on - strand at 2206.832 kb on - strand at 2206.886 kb on - strand, within HER17_RS09975 at 2206.985 kb on - strand, within HER17_RS09975 at 2207.080 kb on + strand at 2207.081 kb on - strand at 2207.103 kb on + strand at 2207.103 kb on + strand at 2207.104 kb on - strand at 2207.104 kb on - strand at 2207.107 kb on + strand at 2207.107 kb on + strand at 2207.107 kb on + strand at 2207.107 kb on + strand at 2207.108 kb on - strand at 2207.109 kb on + strand at 2207.109 kb on + strand at 2207.109 kb on + strand at 2207.109 kb on + strand at 2207.109 kb on + strand at 2207.110 kb on - strand at 2207.110 kb on - strand at 2207.110 kb on - strand at 2207.110 kb on - strand at 2207.168 kb on + strand, within HER17_RS09980 at 2207.262 kb on + strand, within HER17_RS09980 at 2207.262 kb on + strand, within HER17_RS09980 at 2207.262 kb on + strand, within HER17_RS09980 at 2207.262 kb on + strand, within HER17_RS09980 at 2207.263 kb on - strand, within HER17_RS09980 at 2207.345 kb on + strand, within HER17_RS09980 at 2207.346 kb on - strand, within HER17_RS09980 at 2207.393 kb on + strand, within HER17_RS09980 at 2207.393 kb on + strand, within HER17_RS09980 at 2207.394 kb on - strand, within HER17_RS09980 at 2207.394 kb on - strand, within HER17_RS09980 at 2207.394 kb on - strand, within HER17_RS09980 at 2207.431 kb on + strand, within HER17_RS09980 at 2207.432 kb on - strand, within HER17_RS09980 at 2207.774 kb on + strand at 2207.775 kb on - strand at 2207.834 kb on - strand at 2207.894 kb on + strand, within HER17_RS09985 at 2207.894 kb on + strand, within HER17_RS09985 at 2207.894 kb on + strand, within HER17_RS09985 at 2207.895 kb on - strand, within HER17_RS09985 at 2208.001 kb on + strand, within HER17_RS09985 at 2208.002 kb on - strand, within HER17_RS09985 at 2208.002 kb on - strand, within HER17_RS09985 at 2208.057 kb on - strand, within HER17_RS09985
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 2,205,840 + HER17_RS09965 0.84 -1.2 2,205,841 - HER17_RS09965 0.85 -0.1 2,205,841 - HER17_RS09965 0.85 -0.4 2,205,886 - HER17_RS09965 0.89 -1.9 2,206,039 - -0.4 2,206,096 + +0.2 2,206,101 - HER17_RS09970 0.10 +0.8 2,206,191 + HER17_RS09970 0.22 +1.9 2,206,191 + HER17_RS09970 0.22 +0.6 2,206,204 + HER17_RS09970 0.23 -2.7 2,206,298 + HER17_RS09970 0.35 +0.2 2,206,299 - HER17_RS09970 0.35 +0.5 2,206,359 - HER17_RS09970 0.42 -0.7 2,206,584 + HER17_RS09970 0.70 -0.3 2,206,743 + +1.0 2,206,776 + -0.3 2,206,777 - -1.7 2,206,831 + -0.2 2,206,832 - +1.4 2,206,832 - +1.7 2,206,886 - HER17_RS09975 0.22 +0.5 2,206,985 - HER17_RS09975 0.60 +1.4 2,207,080 + +0.3 2,207,081 - -0.2 2,207,103 + -0.8 2,207,103 + +0.3 2,207,104 - +0.9 2,207,104 - -0.2 2,207,107 + +1.0 2,207,107 + +1.2 2,207,107 + -0.1 2,207,107 + +0.1 2,207,108 - -0.6 2,207,109 + +0.2 2,207,109 + +0.9 2,207,109 + +0.0 2,207,109 + -0.6 2,207,109 + +0.3 2,207,110 - -2.5 2,207,110 - +0.0 2,207,110 - -0.4 2,207,110 - +0.0 2,207,168 + HER17_RS09980 0.11 +1.9 2,207,262 + HER17_RS09980 0.25 -0.3 2,207,262 + HER17_RS09980 0.25 +0.4 2,207,262 + HER17_RS09980 0.25 +0.4 2,207,262 + HER17_RS09980 0.25 -0.5 2,207,263 - HER17_RS09980 0.26 -0.0 2,207,345 + HER17_RS09980 0.38 +0.8 2,207,346 - HER17_RS09980 0.38 -1.3 2,207,393 + HER17_RS09980 0.45 -0.1 2,207,393 + HER17_RS09980 0.45 +0.5 2,207,394 - HER17_RS09980 0.46 -0.1 2,207,394 - HER17_RS09980 0.46 +0.3 2,207,394 - HER17_RS09980 0.46 -1.6 2,207,431 + HER17_RS09980 0.51 +1.4 2,207,432 - HER17_RS09980 0.51 -0.1 2,207,774 + -0.3 2,207,775 - -0.3 2,207,834 - -0.3 2,207,894 + HER17_RS09985 0.13 -0.8 2,207,894 + HER17_RS09985 0.13 -0.1 2,207,894 + HER17_RS09985 0.13 -0.7 2,207,895 - HER17_RS09985 0.13 -0.3 2,208,001 + HER17_RS09985 0.22 +0.4 2,208,002 - HER17_RS09985 0.22 +1.6 2,208,002 - HER17_RS09985 0.22 -0.3 2,208,057 - HER17_RS09985 0.27 -0.1
Or see this region's nucleotide sequence