Experiment: M9+polygalacturonate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS01745 and HER17_RS01750 are separated by 111 nucleotides HER17_RS01750 and HER17_RS01755 are separated by 2 nucleotides HER17_RS01755 and HER17_RS01760 overlap by 8 nucleotides HER17_RS01760 and HER17_RS01765 overlap by 4 nucleotides
HER17_RS01745: HER17_RS01745 - peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA, at 410,375 to 412,930
_RS01745
HER17_RS01750: HER17_RS01750 - pilus assembly protein PilM, at 413,042 to 413,893
_RS01750
HER17_RS01755: HER17_RS01755 - PilN domain-containing protein, at 413,896 to 414,465
_RS01755
HER17_RS01760: HER17_RS01760 - hypothetical protein, at 414,458 to 415,045
_RS01760
HER17_RS01765: HER17_RS01765 - DNA utilization family protein, at 415,042 to 415,419
_RS01765
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 412.910 kb on + strand at 412.910 kb on + strand at 412.910 kb on + strand at 412.910 kb on + strand at 412.910 kb on + strand at 412.910 kb on + strand at 412.910 kb on + strand at 412.911 kb on - strand at 412.911 kb on - strand at 412.914 kb on + strand at 412.914 kb on + strand at 412.914 kb on + strand at 412.914 kb on + strand at 412.915 kb on - strand at 412.915 kb on - strand at 412.919 kb on + strand at 412.950 kb on - strand at 412.965 kb on + strand at 412.966 kb on - strand at 412.966 kb on - strand at 412.967 kb on + strand at 412.968 kb on - strand at 412.983 kb on - strand at 412.983 kb on - strand at 412.983 kb on - strand at 412.985 kb on + strand at 412.985 kb on + strand at 412.985 kb on + strand at 412.985 kb on + strand at 412.986 kb on - strand at 412.986 kb on - strand at 412.986 kb on - strand at 412.987 kb on + strand at 412.987 kb on + strand at 412.988 kb on - strand at 412.988 kb on - strand at 412.988 kb on - strand at 412.994 kb on - strand at 412.994 kb on - strand at 413.010 kb on + strand at 413.017 kb on - strand at 413.049 kb on - strand at 413.049 kb on - strand at 413.049 kb on - strand at 413.070 kb on + strand at 413.070 kb on + strand at 413.071 kb on - strand at 413.074 kb on + strand at 413.074 kb on + strand at 413.074 kb on + strand at 413.075 kb on - strand at 413.075 kb on - strand at 413.075 kb on - strand at 413.075 kb on - strand at 413.076 kb on + strand at 413.076 kb on + strand at 413.076 kb on + strand at 413.077 kb on - strand at 413.077 kb on - strand at 413.077 kb on - strand at 413.077 kb on - strand at 413.157 kb on + strand, within HER17_RS01750 at 413.167 kb on + strand, within HER17_RS01750 at 413.168 kb on - strand, within HER17_RS01750 at 413.187 kb on + strand, within HER17_RS01750 at 413.187 kb on + strand, within HER17_RS01750 at 413.187 kb on + strand, within HER17_RS01750 at 413.187 kb on + strand, within HER17_RS01750 at 413.187 kb on + strand, within HER17_RS01750 at 413.188 kb on - strand, within HER17_RS01750 at 413.188 kb on - strand, within HER17_RS01750 at 413.188 kb on - strand, within HER17_RS01750 at 413.298 kb on + strand, within HER17_RS01750 at 413.319 kb on + strand, within HER17_RS01750 at 413.335 kb on + strand, within HER17_RS01750 at 413.336 kb on - strand, within HER17_RS01750 at 413.336 kb on - strand, within HER17_RS01750 at 413.342 kb on + strand, within HER17_RS01750 at 413.343 kb on - strand, within HER17_RS01750 at 413.354 kb on - strand, within HER17_RS01750 at 413.354 kb on - strand, within HER17_RS01750 at 413.365 kb on + strand, within HER17_RS01750 at 413.365 kb on + strand, within HER17_RS01750 at 413.371 kb on - strand, within HER17_RS01750 at 413.371 kb on - strand, within HER17_RS01750 at 413.398 kb on - strand, within HER17_RS01750 at 413.398 kb on - strand, within HER17_RS01750 at 413.398 kb on - strand, within HER17_RS01750 at 413.649 kb on + strand, within HER17_RS01750 at 413.649 kb on + strand, within HER17_RS01750 at 413.650 kb on - strand, within HER17_RS01750 at 413.694 kb on + strand, within HER17_RS01750 at 413.695 kb on - strand, within HER17_RS01750 at 413.699 kb on - strand, within HER17_RS01750 at 413.728 kb on - strand, within HER17_RS01750 at 413.732 kb on + strand, within HER17_RS01750 at 413.732 kb on + strand, within HER17_RS01750 at 413.733 kb on - strand, within HER17_RS01750 at 413.778 kb on + strand, within HER17_RS01750 at 413.779 kb on - strand, within HER17_RS01750 at 413.779 kb on - strand, within HER17_RS01750 at 413.779 kb on - strand, within HER17_RS01750 at 413.782 kb on - strand, within HER17_RS01750 at 413.895 kb on + strand at 413.895 kb on + strand at 413.895 kb on + strand at 413.895 kb on + strand at 413.895 kb on + strand at 413.895 kb on + strand at 413.896 kb on - strand at 413.896 kb on - strand at 413.896 kb on - strand at 413.896 kb on - strand at 413.908 kb on - strand at 413.976 kb on - strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on + strand, within HER17_RS01755 at 413.993 kb on - strand, within HER17_RS01755 at 414.013 kb on + strand, within HER17_RS01755 at 414.062 kb on + strand, within HER17_RS01755 at 414.062 kb on + strand, within HER17_RS01755 at 414.063 kb on - strand, within HER17_RS01755 at 414.097 kb on + strand, within HER17_RS01755 at 414.098 kb on - strand, within HER17_RS01755 at 414.098 kb on - strand, within HER17_RS01755 at 414.326 kb on + strand, within HER17_RS01755 at 414.329 kb on + strand, within HER17_RS01755 at 414.330 kb on - strand, within HER17_RS01755 at 414.354 kb on - strand, within HER17_RS01755 at 414.354 kb on - strand, within HER17_RS01755 at 414.463 kb on + strand at 414.514 kb on + strand at 414.573 kb on + strand, within HER17_RS01760 at 414.574 kb on - strand, within HER17_RS01760 at 414.574 kb on - strand, within HER17_RS01760 at 414.622 kb on - strand, within HER17_RS01760 at 414.680 kb on - strand, within HER17_RS01760 at 414.714 kb on + strand, within HER17_RS01760 at 414.715 kb on - strand, within HER17_RS01760 at 414.715 kb on - strand, within HER17_RS01760 at 414.769 kb on + strand, within HER17_RS01760 at 414.770 kb on - strand, within HER17_RS01760 at 414.807 kb on + strand, within HER17_RS01760 at 414.807 kb on + strand, within HER17_RS01760 at 414.807 kb on + strand, within HER17_RS01760 at 414.881 kb on + strand, within HER17_RS01760 at 414.882 kb on - strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.926 kb on + strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.927 kb on - strand, within HER17_RS01760 at 414.954 kb on + strand, within HER17_RS01760 at 414.993 kb on + strand at 414.994 kb on - strand at 415.014 kb on + strand at 415.015 kb on - strand at 415.051 kb on - strand at 415.051 kb on - strand at 415.146 kb on + strand, within HER17_RS01765 at 415.191 kb on - strand, within HER17_RS01765 at 415.191 kb on - strand, within HER17_RS01765 at 415.191 kb on - strand, within HER17_RS01765 at 415.191 kb on - strand, within HER17_RS01765 at 415.314 kb on + strand, within HER17_RS01765 at 415.314 kb on + strand, within HER17_RS01765 at 415.374 kb on + strand, within HER17_RS01765 at 415.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+polygalacturonate remove 412,910 + +0.3 412,910 + -0.2 412,910 + +0.3 412,910 + +0.2 412,910 + +0.1 412,910 + +0.1 412,910 + +2.5 412,911 - -0.1 412,911 - -0.7 412,914 + +1.1 412,914 + +0.1 412,914 + +0.3 412,914 + +1.1 412,915 - -1.1 412,915 - +0.4 412,919 + +1.3 412,950 - +0.1 412,965 + -0.7 412,966 - -0.3 412,966 - +1.5 412,967 + +0.7 412,968 - +0.2 412,983 - -0.2 412,983 - +0.7 412,983 - +1.3 412,985 + +1.0 412,985 + -0.1 412,985 + +0.2 412,985 + +0.0 412,986 - +0.9 412,986 - -0.3 412,986 - +0.9 412,987 + +0.3 412,987 + +1.2 412,988 - +1.3 412,988 - -0.2 412,988 - -0.5 412,994 - +0.3 412,994 - +0.0 413,010 + -0.2 413,017 - +0.0 413,049 - -2.1 413,049 - -0.4 413,049 - -0.0 413,070 + -0.4 413,070 + -0.1 413,071 - -0.7 413,074 + -0.1 413,074 + -1.1 413,074 + +0.8 413,075 - -0.0 413,075 - +0.5 413,075 - -0.0 413,075 - -1.3 413,076 + +0.6 413,076 + -0.8 413,076 + +0.1 413,077 - -0.4 413,077 - -1.0 413,077 - -0.5 413,077 - -0.3 413,157 + HER17_RS01750 0.13 -0.2 413,167 + HER17_RS01750 0.15 +0.7 413,168 - HER17_RS01750 0.15 -0.1 413,187 + HER17_RS01750 0.17 +0.1 413,187 + HER17_RS01750 0.17 +1.3 413,187 + HER17_RS01750 0.17 +0.2 413,187 + HER17_RS01750 0.17 -0.8 413,187 + HER17_RS01750 0.17 -0.2 413,188 - HER17_RS01750 0.17 -0.5 413,188 - HER17_RS01750 0.17 +0.5 413,188 - HER17_RS01750 0.17 +0.3 413,298 + HER17_RS01750 0.30 -0.1 413,319 + HER17_RS01750 0.33 -0.9 413,335 + HER17_RS01750 0.34 +0.5 413,336 - HER17_RS01750 0.35 +1.2 413,336 - HER17_RS01750 0.35 +0.3 413,342 + HER17_RS01750 0.35 -0.4 413,343 - HER17_RS01750 0.35 +0.7 413,354 - HER17_RS01750 0.37 -1.7 413,354 - HER17_RS01750 0.37 -0.4 413,365 + HER17_RS01750 0.38 -0.3 413,365 + HER17_RS01750 0.38 -0.4 413,371 - HER17_RS01750 0.39 +0.4 413,371 - HER17_RS01750 0.39 +0.7 413,398 - HER17_RS01750 0.42 -0.0 413,398 - HER17_RS01750 0.42 -0.1 413,398 - HER17_RS01750 0.42 +0.3 413,649 + HER17_RS01750 0.71 -0.3 413,649 + HER17_RS01750 0.71 -0.3 413,650 - HER17_RS01750 0.71 -0.5 413,694 + HER17_RS01750 0.77 -0.2 413,695 - HER17_RS01750 0.77 +0.3 413,699 - HER17_RS01750 0.77 -0.7 413,728 - HER17_RS01750 0.81 -0.3 413,732 + HER17_RS01750 0.81 -0.1 413,732 + HER17_RS01750 0.81 +0.3 413,733 - HER17_RS01750 0.81 +0.3 413,778 + HER17_RS01750 0.86 +0.2 413,779 - HER17_RS01750 0.87 -0.7 413,779 - HER17_RS01750 0.87 -0.7 413,779 - HER17_RS01750 0.87 -0.7 413,782 - HER17_RS01750 0.87 -0.2 413,895 + -1.7 413,895 + -1.2 413,895 + -0.4 413,895 + +0.3 413,895 + -0.1 413,895 + +0.1 413,896 - +1.8 413,896 - +0.3 413,896 - -0.8 413,896 - +0.3 413,908 - +0.4 413,976 - HER17_RS01755 0.14 +0.2 413,993 + HER17_RS01755 0.17 -0.2 413,993 + HER17_RS01755 0.17 +1.7 413,993 + HER17_RS01755 0.17 -0.1 413,993 + HER17_RS01755 0.17 +0.2 413,993 + HER17_RS01755 0.17 -0.7 413,993 + HER17_RS01755 0.17 +0.4 413,993 + HER17_RS01755 0.17 +2.7 413,993 - HER17_RS01755 0.17 -0.0 414,013 + HER17_RS01755 0.21 -3.1 414,062 + HER17_RS01755 0.29 -0.1 414,062 + HER17_RS01755 0.29 +0.1 414,063 - HER17_RS01755 0.29 +0.8 414,097 + HER17_RS01755 0.35 +0.4 414,098 - HER17_RS01755 0.35 +0.3 414,098 - HER17_RS01755 0.35 -0.2 414,326 + HER17_RS01755 0.75 +0.5 414,329 + HER17_RS01755 0.76 -1.1 414,330 - HER17_RS01755 0.76 +0.9 414,354 - HER17_RS01755 0.80 -0.6 414,354 - HER17_RS01755 0.80 +0.5 414,463 + -0.1 414,514 + +0.4 414,573 + HER17_RS01760 0.20 +0.6 414,574 - HER17_RS01760 0.20 +1.2 414,574 - HER17_RS01760 0.20 -0.0 414,622 - HER17_RS01760 0.28 +0.3 414,680 - HER17_RS01760 0.38 +0.1 414,714 + HER17_RS01760 0.44 +0.5 414,715 - HER17_RS01760 0.44 +0.6 414,715 - HER17_RS01760 0.44 -0.2 414,769 + HER17_RS01760 0.53 -0.4 414,770 - HER17_RS01760 0.53 +0.9 414,807 + HER17_RS01760 0.59 -1.7 414,807 + HER17_RS01760 0.59 +0.0 414,807 + HER17_RS01760 0.59 +0.2 414,881 + HER17_RS01760 0.72 -0.9 414,882 - HER17_RS01760 0.72 -0.6 414,926 + HER17_RS01760 0.80 -0.8 414,926 + HER17_RS01760 0.80 +2.3 414,926 + HER17_RS01760 0.80 -1.1 414,926 + HER17_RS01760 0.80 +0.5 414,926 + HER17_RS01760 0.80 -0.1 414,926 + HER17_RS01760 0.80 -0.5 414,926 + HER17_RS01760 0.80 +0.2 414,926 + HER17_RS01760 0.80 -0.3 414,926 + HER17_RS01760 0.80 +0.2 414,927 - HER17_RS01760 0.80 -0.4 414,927 - HER17_RS01760 0.80 +0.2 414,927 - HER17_RS01760 0.80 +0.2 414,927 - HER17_RS01760 0.80 +0.5 414,927 - HER17_RS01760 0.80 +0.8 414,927 - HER17_RS01760 0.80 +0.6 414,927 - HER17_RS01760 0.80 +0.2 414,927 - HER17_RS01760 0.80 +0.6 414,927 - HER17_RS01760 0.80 -0.3 414,927 - HER17_RS01760 0.80 +0.3 414,927 - HER17_RS01760 0.80 +0.9 414,954 + HER17_RS01760 0.84 +0.6 414,993 + -0.3 414,994 - +1.0 415,014 + +0.2 415,015 - -0.4 415,051 - -0.4 415,051 - +0.6 415,146 + HER17_RS01765 0.28 +1.0 415,191 - HER17_RS01765 0.39 +0.8 415,191 - HER17_RS01765 0.39 +0.5 415,191 - HER17_RS01765 0.39 +0.7 415,191 - HER17_RS01765 0.39 -0.4 415,314 + HER17_RS01765 0.72 -0.7 415,314 + HER17_RS01765 0.72 +0.2 415,374 + HER17_RS01765 0.88 -0.4 415,418 - -0.2
Or see this region's nucleotide sequence