Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS14555 and HER17_RS14560 overlap by 4 nucleotides HER17_RS14560 and HER17_RS14565 are separated by 66 nucleotides HER17_RS14565 and HER17_RS14570 are separated by 1 nucleotides HER17_RS14570 and HER17_RS14575 are separated by 48 nucleotides
HER17_RS14555: HER17_RS14555 - glycosyltransferase, at 3,274,246 to 3,275,433
_RS14555
HER17_RS14560: HER17_RS14560 - transferase, at 3,275,430 to 3,275,921
_RS14560
HER17_RS14565: HER17_RS14565 - oligosaccharide repeat unit polymerase, at 3,275,988 to 3,277,193
_RS14565
HER17_RS14570: HER17_RS14570 - glycosyltransferase, at 3,277,195 to 3,278,055
_RS14570
HER17_RS14575: HER17_RS14575 - O-antigen translocase, at 3,278,104 to 3,279,369
_RS14575
Position (kb) 3275
3276
3277
3278 Strain fitness (log2 ratio) -1
0
1 at 3275.020 kb on + strand, within HER17_RS14555 at 3275.127 kb on + strand, within HER17_RS14555 at 3275.338 kb on + strand at 3275.519 kb on + strand, within HER17_RS14560 at 3275.553 kb on + strand, within HER17_RS14560 at 3275.594 kb on + strand, within HER17_RS14560 at 3275.935 kb on - strand at 3276.097 kb on + strand at 3276.101 kb on + strand at 3276.143 kb on - strand, within HER17_RS14565 at 3276.242 kb on - strand, within HER17_RS14565 at 3276.282 kb on - strand, within HER17_RS14565 at 3276.282 kb on - strand, within HER17_RS14565 at 3276.300 kb on - strand, within HER17_RS14565 at 3276.327 kb on - strand, within HER17_RS14565 at 3277.405 kb on + strand, within HER17_RS14570 at 3277.516 kb on + strand, within HER17_RS14570 at 3277.599 kb on + strand, within HER17_RS14570 at 3277.650 kb on + strand, within HER17_RS14570
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 3,275,020 + HER17_RS14555 0.65 +0.2 3,275,127 + HER17_RS14555 0.74 -0.5 3,275,338 + -0.7 3,275,519 + HER17_RS14560 0.18 -0.6 3,275,553 + HER17_RS14560 0.25 +0.9 3,275,594 + HER17_RS14560 0.33 +0.6 3,275,935 - -0.5 3,276,097 + -0.6 3,276,101 + -0.7 3,276,143 - HER17_RS14565 0.13 +0.2 3,276,242 - HER17_RS14565 0.21 +0.6 3,276,282 - HER17_RS14565 0.24 +0.3 3,276,282 - HER17_RS14565 0.24 -0.1 3,276,300 - HER17_RS14565 0.26 +0.4 3,276,327 - HER17_RS14565 0.28 -0.4 3,277,405 + HER17_RS14570 0.24 +0.2 3,277,516 + HER17_RS14570 0.37 +0.6 3,277,599 + HER17_RS14570 0.47 +1.3 3,277,650 + HER17_RS14570 0.53 +1.3
Or see this region's nucleotide sequence