Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS08660 and HER17_RS08665 are separated by 128 nucleotides HER17_RS08665 and HER17_RS08670 are separated by 14 nucleotides HER17_RS08670 and HER17_RS08675 overlap by 11 nucleotides HER17_RS08675 and HER17_RS08680 overlap by 1 nucleotides
HER17_RS08660: HER17_RS08660 - beta-ketoacyl-ACP synthase II, at 1,901,408 to 1,902,649
_RS08660
HER17_RS08665: HER17_RS08665 - aminodeoxychorismate lyase, at 1,902,778 to 1,903,575
_RS08665
HER17_RS08670: HER17_RS08670 - endolytic transglycosylase MltG, at 1,903,590 to 1,904,609
_RS08670
HER17_RS08675: HER17_RS08675 - dTMP kinase, at 1,904,599 to 1,905,258
_RS08675
HER17_RS08680: HER17_RS08680 - DNA polymerase III subunit delta', at 1,905,258 to 1,906,268
_RS08680
Position (kb)
1903
1904
1905 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1902.701 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.774 kb on + strand at 1902.775 kb on - strand at 1902.775 kb on - strand at 1902.775 kb on - strand at 1902.777 kb on + strand at 1902.777 kb on + strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.778 kb on - strand at 1902.789 kb on + strand at 1902.831 kb on + strand at 1902.839 kb on - strand at 1902.870 kb on - strand, within HER17_RS08665 at 1902.936 kb on - strand, within HER17_RS08665 at 1903.051 kb on + strand, within HER17_RS08665 at 1903.051 kb on + strand, within HER17_RS08665 at 1903.097 kb on + strand, within HER17_RS08665 at 1903.102 kb on + strand, within HER17_RS08665 at 1903.115 kb on - strand, within HER17_RS08665 at 1903.115 kb on - strand, within HER17_RS08665 at 1903.138 kb on - strand, within HER17_RS08665 at 1903.140 kb on + strand, within HER17_RS08665 at 1903.166 kb on + strand, within HER17_RS08665 at 1903.194 kb on - strand, within HER17_RS08665 at 1903.339 kb on + strand, within HER17_RS08665 at 1903.339 kb on + strand, within HER17_RS08665 at 1903.340 kb on - strand, within HER17_RS08665 at 1903.342 kb on - strand, within HER17_RS08665 at 1903.389 kb on + strand, within HER17_RS08665 at 1903.425 kb on - strand, within HER17_RS08665 at 1903.589 kb on + strand at 1903.619 kb on + strand at 1903.772 kb on + strand, within HER17_RS08670 at 1903.845 kb on + strand, within HER17_RS08670 at 1903.853 kb on + strand, within HER17_RS08670 at 1903.871 kb on - strand, within HER17_RS08670 at 1903.871 kb on - strand, within HER17_RS08670 at 1903.980 kb on + strand, within HER17_RS08670 at 1903.980 kb on + strand, within HER17_RS08670 at 1903.980 kb on + strand, within HER17_RS08670 at 1903.980 kb on + strand, within HER17_RS08670 at 1903.981 kb on - strand, within HER17_RS08670 at 1903.982 kb on + strand, within HER17_RS08670 at 1903.983 kb on - strand, within HER17_RS08670 at 1904.078 kb on + strand, within HER17_RS08670 at 1904.082 kb on + strand, within HER17_RS08670 at 1904.082 kb on + strand, within HER17_RS08670 at 1904.088 kb on + strand, within HER17_RS08670 at 1904.089 kb on - strand, within HER17_RS08670 at 1904.161 kb on + strand, within HER17_RS08670 at 1904.192 kb on + strand, within HER17_RS08670 at 1904.193 kb on - strand, within HER17_RS08670 at 1904.334 kb on + strand, within HER17_RS08670 at 1904.380 kb on + strand, within HER17_RS08670 at 1904.402 kb on + strand, within HER17_RS08670 at 1904.402 kb on + strand, within HER17_RS08670 at 1904.402 kb on + strand, within HER17_RS08670 at 1904.402 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.406 kb on + strand, within HER17_RS08670 at 1904.422 kb on + strand, within HER17_RS08670 at 1904.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 1,902,701 + +0.5 1,902,774 + +0.7 1,902,774 + -2.3 1,902,774 + +0.3 1,902,775 - +0.4 1,902,775 - +0.3 1,902,775 - +0.2 1,902,777 + +0.1 1,902,777 + -1.0 1,902,778 - -0.1 1,902,778 - -0.5 1,902,778 - -1.0 1,902,778 - -1.0 1,902,789 + -0.2 1,902,831 + -1.8 1,902,839 - +0.2 1,902,870 - HER17_RS08665 0.12 -0.1 1,902,936 - HER17_RS08665 0.20 -0.3 1,903,051 + HER17_RS08665 0.34 +0.0 1,903,051 + HER17_RS08665 0.34 -0.8 1,903,097 + HER17_RS08665 0.40 -0.9 1,903,102 + HER17_RS08665 0.41 +0.3 1,903,115 - HER17_RS08665 0.42 -0.5 1,903,115 - HER17_RS08665 0.42 +0.2 1,903,138 - HER17_RS08665 0.45 +0.4 1,903,140 + HER17_RS08665 0.45 -0.4 1,903,166 + HER17_RS08665 0.49 -1.0 1,903,194 - HER17_RS08665 0.52 +0.3 1,903,339 + HER17_RS08665 0.70 -0.2 1,903,339 + HER17_RS08665 0.70 -1.4 1,903,340 - HER17_RS08665 0.70 -0.3 1,903,342 - HER17_RS08665 0.71 +0.9 1,903,389 + HER17_RS08665 0.77 +0.5 1,903,425 - HER17_RS08665 0.81 +0.3 1,903,589 + +0.2 1,903,619 + +0.5 1,903,772 + HER17_RS08670 0.18 -2.0 1,903,845 + HER17_RS08670 0.25 +0.3 1,903,853 + HER17_RS08670 0.26 +0.6 1,903,871 - HER17_RS08670 0.28 -0.0 1,903,871 - HER17_RS08670 0.28 -1.1 1,903,980 + HER17_RS08670 0.38 -0.1 1,903,980 + HER17_RS08670 0.38 -0.1 1,903,980 + HER17_RS08670 0.38 +1.1 1,903,980 + HER17_RS08670 0.38 -0.8 1,903,981 - HER17_RS08670 0.38 -1.7 1,903,982 + HER17_RS08670 0.38 -0.6 1,903,983 - HER17_RS08670 0.39 -0.7 1,904,078 + HER17_RS08670 0.48 +0.7 1,904,082 + HER17_RS08670 0.48 -1.0 1,904,082 + HER17_RS08670 0.48 +1.3 1,904,088 + HER17_RS08670 0.49 +0.3 1,904,089 - HER17_RS08670 0.49 +2.3 1,904,161 + HER17_RS08670 0.56 +0.6 1,904,192 + HER17_RS08670 0.59 +1.9 1,904,193 - HER17_RS08670 0.59 -0.4 1,904,334 + HER17_RS08670 0.73 -0.2 1,904,380 + HER17_RS08670 0.77 +0.1 1,904,402 + HER17_RS08670 0.80 -0.3 1,904,402 + HER17_RS08670 0.80 -1.3 1,904,402 + HER17_RS08670 0.80 +0.5 1,904,402 + HER17_RS08670 0.80 +0.5 1,904,406 + HER17_RS08670 0.80 -0.1 1,904,406 + HER17_RS08670 0.80 +0.9 1,904,406 + HER17_RS08670 0.80 +0.7 1,904,406 + HER17_RS08670 0.80 +0.3 1,904,406 + HER17_RS08670 0.80 -1.6 1,904,406 + HER17_RS08670 0.80 -0.4 1,904,406 + HER17_RS08670 0.80 -0.4 1,904,422 + HER17_RS08670 0.82 +0.1 1,904,540 + +0.1
Or see this region's nucleotide sequence