Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS08445 and HER17_RS08450 are separated by 77 nucleotides HER17_RS08450 and HER17_RS08455 are separated by 137 nucleotides HER17_RS08455 and HER17_RS08460 are separated by 194 nucleotides HER17_RS08460 and HER17_RS08465 are separated by 82 nucleotides
HER17_RS08445: HER17_RS08445 - DNA helicase IV, at 1,862,221 to 1,864,278
_RS08445
HER17_RS08450: HER17_RS08450 - methylglyoxal synthase, at 1,864,356 to 1,864,814
_RS08450
HER17_RS08455: HER17_RS08455 - DUF2057 family protein, at 1,864,952 to 1,865,476
_RS08455
HER17_RS08460: HER17_RS08460 - CoA-binding protein, at 1,865,671 to 1,866,084
_RS08460
HER17_RS08465: HER17_RS08465 - heat shock protein HspQ, at 1,866,167 to 1,866,475
_RS08465
Position (kb)
1864
1865
1866 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1864.030 kb on + strand, within HER17_RS08445 at 1864.031 kb on - strand, within HER17_RS08445 at 1864.113 kb on - strand at 1864.154 kb on + strand at 1864.154 kb on + strand at 1864.159 kb on + strand at 1864.159 kb on + strand at 1864.160 kb on - strand at 1864.247 kb on + strand at 1864.267 kb on - strand at 1864.294 kb on - strand at 1864.322 kb on + strand at 1864.322 kb on + strand at 1864.322 kb on + strand at 1864.322 kb on + strand at 1864.325 kb on + strand at 1864.325 kb on + strand at 1864.339 kb on - strand at 1864.339 kb on - strand at 1864.379 kb on - strand at 1864.387 kb on + strand at 1864.418 kb on + strand, within HER17_RS08450 at 1864.419 kb on - strand, within HER17_RS08450 at 1864.422 kb on + strand, within HER17_RS08450 at 1864.422 kb on + strand, within HER17_RS08450 at 1864.422 kb on + strand, within HER17_RS08450 at 1864.423 kb on - strand, within HER17_RS08450 at 1864.433 kb on + strand, within HER17_RS08450 at 1864.464 kb on - strand, within HER17_RS08450 at 1864.509 kb on + strand, within HER17_RS08450 at 1864.509 kb on + strand, within HER17_RS08450 at 1864.510 kb on - strand, within HER17_RS08450 at 1864.560 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.623 kb on + strand, within HER17_RS08450 at 1864.624 kb on - strand, within HER17_RS08450 at 1864.624 kb on - strand, within HER17_RS08450 at 1864.688 kb on - strand, within HER17_RS08450 at 1864.688 kb on - strand, within HER17_RS08450 at 1864.721 kb on + strand, within HER17_RS08450 at 1864.734 kb on + strand, within HER17_RS08450 at 1864.735 kb on - strand, within HER17_RS08450 at 1864.749 kb on - strand, within HER17_RS08450 at 1864.749 kb on - strand, within HER17_RS08450 at 1864.796 kb on + strand at 1864.818 kb on + strand at 1864.837 kb on + strand at 1864.837 kb on + strand at 1864.837 kb on + strand at 1864.838 kb on - strand at 1864.841 kb on - strand at 1864.841 kb on - strand at 1864.859 kb on + strand at 1864.860 kb on - strand at 1864.860 kb on - strand at 1864.897 kb on + strand at 1865.022 kb on + strand, within HER17_RS08455 at 1865.023 kb on - strand, within HER17_RS08455 at 1865.042 kb on + strand, within HER17_RS08455 at 1865.043 kb on - strand, within HER17_RS08455 at 1865.043 kb on - strand, within HER17_RS08455 at 1865.043 kb on - strand, within HER17_RS08455 at 1865.051 kb on + strand, within HER17_RS08455 at 1865.051 kb on + strand, within HER17_RS08455 at 1865.051 kb on + strand, within HER17_RS08455 at 1865.052 kb on - strand, within HER17_RS08455 at 1865.117 kb on + strand, within HER17_RS08455 at 1865.117 kb on + strand, within HER17_RS08455 at 1865.118 kb on - strand, within HER17_RS08455 at 1865.118 kb on - strand, within HER17_RS08455 at 1865.118 kb on - strand, within HER17_RS08455 at 1865.125 kb on - strand, within HER17_RS08455 at 1865.125 kb on - strand, within HER17_RS08455 at 1865.141 kb on - strand, within HER17_RS08455 at 1865.182 kb on + strand, within HER17_RS08455 at 1865.182 kb on + strand, within HER17_RS08455 at 1865.225 kb on - strand, within HER17_RS08455 at 1865.241 kb on + strand, within HER17_RS08455 at 1865.242 kb on - strand, within HER17_RS08455 at 1865.276 kb on - strand, within HER17_RS08455 at 1865.397 kb on - strand, within HER17_RS08455 at 1865.428 kb on + strand at 1865.428 kb on + strand at 1865.429 kb on - strand at 1865.432 kb on + strand at 1865.476 kb on + strand at 1865.477 kb on - strand at 1865.496 kb on + strand at 1865.497 kb on - strand at 1865.497 kb on - strand at 1865.578 kb on + strand at 1865.578 kb on + strand at 1865.578 kb on + strand at 1865.578 kb on + strand at 1865.579 kb on - strand at 1865.579 kb on - strand at 1865.579 kb on - strand at 1865.579 kb on - strand at 1865.595 kb on + strand at 1865.595 kb on + strand at 1865.595 kb on + strand at 1865.596 kb on - strand at 1865.602 kb on - strand at 1865.616 kb on + strand at 1865.616 kb on + strand at 1865.623 kb on - strand at 1865.641 kb on + strand at 1865.641 kb on + strand at 1865.641 kb on + strand at 1865.641 kb on + strand at 1865.642 kb on - strand at 1865.697 kb on - strand at 1865.758 kb on + strand, within HER17_RS08460 at 1865.781 kb on + strand, within HER17_RS08460 at 1865.837 kb on + strand, within HER17_RS08460 at 1865.868 kb on + strand, within HER17_RS08460 at 1865.925 kb on + strand, within HER17_RS08460 at 1866.083 kb on - strand at 1866.094 kb on - strand at 1866.094 kb on - strand at 1866.094 kb on - strand at 1866.096 kb on + strand at 1866.128 kb on - strand at 1866.276 kb on + strand, within HER17_RS08465 at 1866.276 kb on + strand, within HER17_RS08465 at 1866.276 kb on + strand, within HER17_RS08465 at 1866.276 kb on + strand, within HER17_RS08465 at 1866.276 kb on + strand, within HER17_RS08465 at 1866.276 kb on + strand, within HER17_RS08465 at 1866.277 kb on - strand, within HER17_RS08465 at 1866.277 kb on - strand, within HER17_RS08465 at 1866.280 kb on + strand, within HER17_RS08465 at 1866.281 kb on - strand, within HER17_RS08465 at 1866.281 kb on - strand, within HER17_RS08465 at 1866.349 kb on + strand, within HER17_RS08465 at 1866.355 kb on + strand, within HER17_RS08465 at 1866.387 kb on + strand, within HER17_RS08465 at 1866.388 kb on - strand, within HER17_RS08465 at 1866.407 kb on - strand, within HER17_RS08465 at 1866.434 kb on - strand, within HER17_RS08465 at 1866.475 kb on + strand at 1866.475 kb on + strand at 1866.476 kb on - strand at 1866.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 1,864,030 + HER17_RS08445 0.88 +0.1 1,864,031 - HER17_RS08445 0.88 -0.2 1,864,113 - +1.6 1,864,154 + -1.0 1,864,154 + +0.2 1,864,159 + -0.5 1,864,159 + +0.2 1,864,160 - +0.3 1,864,247 + -0.6 1,864,267 - -0.6 1,864,294 - +1.0 1,864,322 + -0.2 1,864,322 + +1.0 1,864,322 + +0.2 1,864,322 + +0.2 1,864,325 + +0.6 1,864,325 + +0.8 1,864,339 - +0.1 1,864,339 - -1.1 1,864,379 - -0.1 1,864,387 + -0.1 1,864,418 + HER17_RS08450 0.14 -0.2 1,864,419 - HER17_RS08450 0.14 -0.3 1,864,422 + HER17_RS08450 0.14 -0.0 1,864,422 + HER17_RS08450 0.14 +0.4 1,864,422 + HER17_RS08450 0.14 -1.6 1,864,423 - HER17_RS08450 0.15 -0.1 1,864,433 + HER17_RS08450 0.17 -0.0 1,864,464 - HER17_RS08450 0.24 +0.1 1,864,509 + HER17_RS08450 0.33 -0.9 1,864,509 + HER17_RS08450 0.33 -0.2 1,864,510 - HER17_RS08450 0.34 +0.6 1,864,560 + HER17_RS08450 0.44 +1.2 1,864,623 + HER17_RS08450 0.58 +0.0 1,864,623 + HER17_RS08450 0.58 -0.4 1,864,623 + HER17_RS08450 0.58 +0.2 1,864,623 + HER17_RS08450 0.58 -0.5 1,864,623 + HER17_RS08450 0.58 -0.5 1,864,623 + HER17_RS08450 0.58 +1.0 1,864,624 - HER17_RS08450 0.58 +0.6 1,864,624 - HER17_RS08450 0.58 +0.0 1,864,688 - HER17_RS08450 0.72 -0.2 1,864,688 - HER17_RS08450 0.72 -0.4 1,864,721 + HER17_RS08450 0.80 -0.9 1,864,734 + HER17_RS08450 0.82 -0.1 1,864,735 - HER17_RS08450 0.83 -0.1 1,864,749 - HER17_RS08450 0.86 +2.7 1,864,749 - HER17_RS08450 0.86 +0.4 1,864,796 + -1.3 1,864,818 + -0.7 1,864,837 + +0.6 1,864,837 + -1.1 1,864,837 + +0.4 1,864,838 - -0.3 1,864,841 - +0.4 1,864,841 - +1.0 1,864,859 + +0.1 1,864,860 - -0.0 1,864,860 - -0.5 1,864,897 + +0.7 1,865,022 + HER17_RS08455 0.13 -0.3 1,865,023 - HER17_RS08455 0.14 +1.0 1,865,042 + HER17_RS08455 0.17 -1.2 1,865,043 - HER17_RS08455 0.17 -0.4 1,865,043 - HER17_RS08455 0.17 -0.7 1,865,043 - HER17_RS08455 0.17 +0.3 1,865,051 + HER17_RS08455 0.19 -1.4 1,865,051 + HER17_RS08455 0.19 -0.5 1,865,051 + HER17_RS08455 0.19 -0.5 1,865,052 - HER17_RS08455 0.19 +0.6 1,865,117 + HER17_RS08455 0.31 +0.2 1,865,117 + HER17_RS08455 0.31 +0.9 1,865,118 - HER17_RS08455 0.32 -0.6 1,865,118 - HER17_RS08455 0.32 -0.4 1,865,118 - HER17_RS08455 0.32 -2.0 1,865,125 - HER17_RS08455 0.33 -1.4 1,865,125 - HER17_RS08455 0.33 +0.4 1,865,141 - HER17_RS08455 0.36 +0.2 1,865,182 + HER17_RS08455 0.44 +1.4 1,865,182 + HER17_RS08455 0.44 -0.6 1,865,225 - HER17_RS08455 0.52 -0.4 1,865,241 + HER17_RS08455 0.55 +0.1 1,865,242 - HER17_RS08455 0.55 -0.4 1,865,276 - HER17_RS08455 0.62 +0.1 1,865,397 - HER17_RS08455 0.85 +0.3 1,865,428 + +0.1 1,865,428 + -0.8 1,865,429 - -1.0 1,865,432 + -1.0 1,865,476 + +0.4 1,865,477 - +0.4 1,865,496 + +0.2 1,865,497 - -1.3 1,865,497 - -0.9 1,865,578 + +0.1 1,865,578 + -0.1 1,865,578 + -0.4 1,865,578 + -0.4 1,865,579 - -1.0 1,865,579 - +0.5 1,865,579 - -0.6 1,865,579 - +0.0 1,865,595 + -0.9 1,865,595 + +0.1 1,865,595 + +0.3 1,865,596 - -1.6 1,865,602 - -0.4 1,865,616 + +0.7 1,865,616 + -0.0 1,865,623 - -0.9 1,865,641 + +0.1 1,865,641 + +0.3 1,865,641 + +0.4 1,865,641 + +0.2 1,865,642 - -0.3 1,865,697 - -1.3 1,865,758 + HER17_RS08460 0.21 -0.7 1,865,781 + HER17_RS08460 0.27 -0.6 1,865,837 + HER17_RS08460 0.40 +0.1 1,865,868 + HER17_RS08460 0.48 +0.1 1,865,925 + HER17_RS08460 0.61 -0.5 1,866,083 - -0.1 1,866,094 - +0.6 1,866,094 - -1.6 1,866,094 - -1.6 1,866,096 + +0.0 1,866,128 - +0.3 1,866,276 + HER17_RS08465 0.35 +0.6 1,866,276 + HER17_RS08465 0.35 +1.1 1,866,276 + HER17_RS08465 0.35 +0.3 1,866,276 + HER17_RS08465 0.35 +0.1 1,866,276 + HER17_RS08465 0.35 -0.8 1,866,276 + HER17_RS08465 0.35 -0.5 1,866,277 - HER17_RS08465 0.36 +0.0 1,866,277 - HER17_RS08465 0.36 -0.6 1,866,280 + HER17_RS08465 0.37 -0.3 1,866,281 - HER17_RS08465 0.37 -0.3 1,866,281 - HER17_RS08465 0.37 -0.5 1,866,349 + HER17_RS08465 0.59 +0.4 1,866,355 + HER17_RS08465 0.61 +0.8 1,866,387 + HER17_RS08465 0.71 +0.5 1,866,388 - HER17_RS08465 0.72 +0.5 1,866,407 - HER17_RS08465 0.78 -1.7 1,866,434 - HER17_RS08465 0.86 -0.6 1,866,475 + +0.5 1,866,475 + +0.4 1,866,476 - +0.3 1,866,476 - -0.1
Or see this region's nucleotide sequence