Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS18210 and HER17_RS18215 are separated by 25 nucleotides HER17_RS18215 and HER17_RS18220 are separated by 21 nucleotides HER17_RS18220 and HER17_RS18225 are separated by 218 nucleotides
HER17_RS18210: HER17_RS18210 - translation initiation factor IF-2, at 4,100,333 to 4,103,032
_RS18210
HER17_RS18215: HER17_RS18215 - transcription termination factor NusA, at 4,103,058 to 4,104,587
_RS18215
HER17_RS18220: HER17_RS18220 - ribosome maturation factor RimP, at 4,104,609 to 4,105,061
_RS18220
HER17_RS18225: HER17_RS18225 - tRNA-Met, at 4,105,280 to 4,105,356
_RS18225
Position (kb)
4103
4104
4105 Strain fitness (log2 ratio)
-2
-1
0
1 at 4103.050 kb on - strand at 4103.088 kb on - strand at 4103.088 kb on - strand at 4103.091 kb on - strand at 4103.091 kb on - strand at 4103.142 kb on - strand at 4105.410 kb on + strand at 4105.435 kb on - strand at 4105.435 kb on - strand at 4105.442 kb on + strand at 4105.478 kb on - strand at 4105.501 kb on + strand at 4105.501 kb on + strand at 4105.501 kb on + strand at 4105.511 kb on + strand at 4105.511 kb on + strand at 4105.542 kb on + strand at 4105.543 kb on - strand at 4105.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 4,103,050 - +1.4 4,103,088 - +0.3 4,103,088 - -1.0 4,103,091 - -0.2 4,103,091 - +0.7 4,103,142 - -0.4 4,105,410 + -0.7 4,105,435 - +0.1 4,105,435 - +0.6 4,105,442 + +0.7 4,105,478 - +0.5 4,105,501 + +0.1 4,105,501 + -0.0 4,105,501 + -0.4 4,105,511 + +0.0 4,105,511 + -2.2 4,105,542 + +0.4 4,105,543 - +0.7 4,105,587 - +0.4
Or see this region's nucleotide sequence