Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS14370 and HER17_RS14375 are separated by 21 nucleotides HER17_RS14375 and HER17_RS14380 are separated by 77 nucleotides HER17_RS14380 and HER17_RS14385 overlap by 1 nucleotides HER17_RS14385 and HER17_RS14390 are separated by 102 nucleotides
HER17_RS14370: HER17_RS14370 - iron ABC transporter permease, at 3,231,161 to 3,232,858
_RS14370
HER17_RS14375: HER17_RS14375 - ABC transporter ATP-binding protein, at 3,232,880 to 3,233,569
_RS14375
HER17_RS14380: HER17_RS14380 - type II toxin-antitoxin system ParD family antitoxin, at 3,233,647 to 3,233,925
_RS14380
HER17_RS14385: HER17_RS14385 - type II toxin-antitoxin system RelE/ParE family toxin, at 3,233,925 to 3,234,245
_RS14385
HER17_RS14390: HER17_RS14390 - 5-deoxy-glucuronate isomerase, at 3,234,348 to 3,235,184
_RS14390
Position (kb)
3233
3234 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3232.754 kb on + strand at 3232.755 kb on - strand at 3232.755 kb on - strand at 3232.906 kb on + strand at 3232.906 kb on + strand at 3232.937 kb on - strand at 3232.937 kb on - strand at 3232.937 kb on - strand at 3232.937 kb on - strand at 3232.937 kb on - strand at 3233.020 kb on + strand, within HER17_RS14375 at 3233.020 kb on + strand, within HER17_RS14375 at 3233.159 kb on + strand, within HER17_RS14375 at 3233.160 kb on - strand, within HER17_RS14375 at 3233.160 kb on - strand, within HER17_RS14375 at 3233.194 kb on + strand, within HER17_RS14375 at 3233.194 kb on + strand, within HER17_RS14375 at 3233.194 kb on + strand, within HER17_RS14375 at 3233.326 kb on + strand, within HER17_RS14375 at 3233.326 kb on + strand, within HER17_RS14375 at 3233.470 kb on - strand, within HER17_RS14375 at 3233.470 kb on - strand, within HER17_RS14375 at 3233.512 kb on + strand at 3233.513 kb on - strand at 3233.513 kb on - strand at 3233.545 kb on + strand at 3233.545 kb on + strand at 3233.546 kb on - strand at 3233.546 kb on - strand at 3233.546 kb on - strand at 3233.546 kb on - strand at 3233.609 kb on + strand at 3233.610 kb on - strand at 3233.631 kb on - strand at 3233.633 kb on + strand at 3233.633 kb on + strand at 3233.671 kb on - strand at 3233.671 kb on - strand at 3233.878 kb on - strand, within HER17_RS14380 at 3233.923 kb on + strand at 3233.924 kb on - strand at 3233.932 kb on - strand at 3233.932 kb on - strand at 3233.985 kb on + strand, within HER17_RS14385 at 3234.045 kb on + strand, within HER17_RS14385 at 3234.120 kb on - strand, within HER17_RS14385 at 3234.149 kb on + strand, within HER17_RS14385 at 3234.244 kb on - strand at 3234.250 kb on - strand at 3234.257 kb on + strand at 3234.278 kb on + strand at 3234.283 kb on + strand at 3234.283 kb on + strand at 3234.303 kb on + strand at 3234.327 kb on + strand at 3234.327 kb on + strand at 3234.327 kb on + strand at 3234.328 kb on - strand at 3234.328 kb on - strand at 3234.349 kb on + strand at 3234.366 kb on - strand at 3234.380 kb on - strand at 3234.460 kb on + strand, within HER17_RS14390 at 3234.460 kb on + strand, within HER17_RS14390 at 3234.460 kb on + strand, within HER17_RS14390 at 3234.461 kb on - strand, within HER17_RS14390 at 3234.463 kb on + strand, within HER17_RS14390 at 3234.464 kb on - strand, within HER17_RS14390 at 3234.472 kb on + strand, within HER17_RS14390 at 3234.473 kb on - strand, within HER17_RS14390 at 3234.473 kb on - strand, within HER17_RS14390 at 3234.473 kb on - strand, within HER17_RS14390 at 3234.500 kb on - strand, within HER17_RS14390 at 3234.527 kb on + strand, within HER17_RS14390 at 3234.532 kb on + strand, within HER17_RS14390 at 3234.552 kb on + strand, within HER17_RS14390 at 3234.552 kb on + strand, within HER17_RS14390 at 3234.552 kb on + strand, within HER17_RS14390 at 3234.552 kb on + strand, within HER17_RS14390 at 3234.552 kb on + strand, within HER17_RS14390 at 3234.571 kb on + strand, within HER17_RS14390 at 3234.616 kb on + strand, within HER17_RS14390 at 3234.619 kb on + strand, within HER17_RS14390 at 3234.619 kb on + strand, within HER17_RS14390 at 3234.619 kb on + strand, within HER17_RS14390 at 3234.620 kb on - strand, within HER17_RS14390 at 3234.620 kb on - strand, within HER17_RS14390 at 3234.635 kb on - strand, within HER17_RS14390 at 3234.706 kb on + strand, within HER17_RS14390 at 3234.706 kb on + strand, within HER17_RS14390 at 3234.756 kb on - strand, within HER17_RS14390 at 3234.756 kb on - strand, within HER17_RS14390 at 3234.758 kb on + strand, within HER17_RS14390 at 3234.766 kb on + strand, within HER17_RS14390 at 3234.766 kb on + strand, within HER17_RS14390 at 3234.766 kb on + strand, within HER17_RS14390 at 3234.767 kb on - strand, within HER17_RS14390 at 3234.767 kb on - strand, within HER17_RS14390 at 3234.920 kb on - strand, within HER17_RS14390
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 3,232,754 + +1.0 3,232,755 - -0.2 3,232,755 - -0.6 3,232,906 + -1.0 3,232,906 + +0.3 3,232,937 - +0.1 3,232,937 - +0.2 3,232,937 - -0.2 3,232,937 - +0.1 3,232,937 - -0.2 3,233,020 + HER17_RS14375 0.20 -0.0 3,233,020 + HER17_RS14375 0.20 -0.5 3,233,159 + HER17_RS14375 0.40 -0.4 3,233,160 - HER17_RS14375 0.41 +0.1 3,233,160 - HER17_RS14375 0.41 -0.1 3,233,194 + HER17_RS14375 0.46 +0.4 3,233,194 + HER17_RS14375 0.46 +0.1 3,233,194 + HER17_RS14375 0.46 +0.4 3,233,326 + HER17_RS14375 0.65 -1.1 3,233,326 + HER17_RS14375 0.65 -0.0 3,233,470 - HER17_RS14375 0.86 +0.9 3,233,470 - HER17_RS14375 0.86 -1.2 3,233,512 + -0.3 3,233,513 - -0.3 3,233,513 - +0.1 3,233,545 + -1.3 3,233,545 + -0.8 3,233,546 - +1.9 3,233,546 - -0.7 3,233,546 - -0.8 3,233,546 - -1.5 3,233,609 + -0.7 3,233,610 - -0.8 3,233,631 - +0.4 3,233,633 + -0.2 3,233,633 + -0.5 3,233,671 - +1.2 3,233,671 - +1.9 3,233,878 - HER17_RS14380 0.83 -0.1 3,233,923 + +0.1 3,233,924 - +0.5 3,233,932 - -0.5 3,233,932 - -0.3 3,233,985 + HER17_RS14385 0.19 -0.1 3,234,045 + HER17_RS14385 0.37 -0.1 3,234,120 - HER17_RS14385 0.61 -1.4 3,234,149 + HER17_RS14385 0.70 -0.1 3,234,244 - -0.3 3,234,250 - +1.9 3,234,257 + -0.1 3,234,278 + -0.0 3,234,283 + +0.2 3,234,283 + -0.3 3,234,303 + -0.1 3,234,327 + -0.3 3,234,327 + -0.4 3,234,327 + +0.1 3,234,328 - -0.2 3,234,328 - -0.1 3,234,349 + -1.7 3,234,366 - +0.3 3,234,380 - -0.3 3,234,460 + HER17_RS14390 0.13 +0.7 3,234,460 + HER17_RS14390 0.13 -0.9 3,234,460 + HER17_RS14390 0.13 +0.9 3,234,461 - HER17_RS14390 0.14 +0.3 3,234,463 + HER17_RS14390 0.14 -0.9 3,234,464 - HER17_RS14390 0.14 -0.1 3,234,472 + HER17_RS14390 0.15 +0.3 3,234,473 - HER17_RS14390 0.15 -0.1 3,234,473 - HER17_RS14390 0.15 -1.3 3,234,473 - HER17_RS14390 0.15 +0.9 3,234,500 - HER17_RS14390 0.18 -0.6 3,234,527 + HER17_RS14390 0.21 -0.8 3,234,532 + HER17_RS14390 0.22 -2.0 3,234,552 + HER17_RS14390 0.24 -0.1 3,234,552 + HER17_RS14390 0.24 -1.6 3,234,552 + HER17_RS14390 0.24 -1.0 3,234,552 + HER17_RS14390 0.24 +0.0 3,234,552 + HER17_RS14390 0.24 -0.1 3,234,571 + HER17_RS14390 0.27 +0.5 3,234,616 + HER17_RS14390 0.32 -0.8 3,234,619 + HER17_RS14390 0.32 +0.9 3,234,619 + HER17_RS14390 0.32 -0.4 3,234,619 + HER17_RS14390 0.32 +0.3 3,234,620 - HER17_RS14390 0.32 -0.3 3,234,620 - HER17_RS14390 0.32 +0.8 3,234,635 - HER17_RS14390 0.34 -1.4 3,234,706 + HER17_RS14390 0.43 +0.8 3,234,706 + HER17_RS14390 0.43 -1.0 3,234,756 - HER17_RS14390 0.49 -0.2 3,234,756 - HER17_RS14390 0.49 +0.5 3,234,758 + HER17_RS14390 0.49 +0.2 3,234,766 + HER17_RS14390 0.50 +0.4 3,234,766 + HER17_RS14390 0.50 +0.5 3,234,766 + HER17_RS14390 0.50 +1.6 3,234,767 - HER17_RS14390 0.50 -0.1 3,234,767 - HER17_RS14390 0.50 +0.7 3,234,920 - HER17_RS14390 0.68 -0.3
Or see this region's nucleotide sequence