Experiment: M9+starch
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS06465 and HER17_RS06470 are separated by 517 nucleotides HER17_RS06470 and HER17_RS06475 overlap by 4 nucleotides HER17_RS06475 and HER17_RS06480 are separated by 178 nucleotides
HER17_RS06465: HER17_RS06465 - HAAAP family serine/threonine permease, at 1,439,993 to 1,441,282
_RS06465
HER17_RS06470: HER17_RS06470 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,441,800 to 1,442,870
_RS06470
HER17_RS06475: HER17_RS06475 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 1,442,867 to 1,443,376
_RS06475
HER17_RS06480: HER17_RS06480 - UDP-2,3-diacylglucosamine diphosphatase, at 1,443,555 to 1,444,277
_RS06480
Position (kb)
1441
1442
1443 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1440.829 kb on + strand, within HER17_RS06465 at 1440.829 kb on + strand, within HER17_RS06465 at 1440.829 kb on + strand, within HER17_RS06465 at 1440.830 kb on - strand, within HER17_RS06465 at 1440.830 kb on - strand, within HER17_RS06465 at 1440.852 kb on - strand, within HER17_RS06465 at 1440.910 kb on + strand, within HER17_RS06465 at 1440.911 kb on - strand, within HER17_RS06465 at 1440.914 kb on + strand, within HER17_RS06465 at 1440.915 kb on - strand, within HER17_RS06465 at 1440.938 kb on - strand, within HER17_RS06465 at 1441.089 kb on + strand, within HER17_RS06465 at 1441.092 kb on + strand, within HER17_RS06465 at 1441.092 kb on + strand, within HER17_RS06465 at 1441.092 kb on + strand, within HER17_RS06465 at 1441.092 kb on + strand, within HER17_RS06465 at 1441.093 kb on - strand, within HER17_RS06465 at 1441.093 kb on - strand, within HER17_RS06465 at 1441.124 kb on - strand, within HER17_RS06465 at 1441.163 kb on + strand at 1441.169 kb on + strand at 1441.170 kb on - strand at 1441.228 kb on + strand at 1441.282 kb on + strand at 1441.282 kb on + strand at 1441.283 kb on - strand at 1441.284 kb on + strand at 1441.284 kb on + strand at 1441.308 kb on - strand at 1441.308 kb on - strand at 1441.310 kb on + strand at 1441.311 kb on - strand at 1441.414 kb on - strand at 1441.414 kb on - strand at 1441.433 kb on + strand at 1441.454 kb on + strand at 1441.454 kb on + strand at 1441.455 kb on - strand at 1441.455 kb on - strand at 1441.455 kb on - strand at 1441.455 kb on - strand at 1441.555 kb on + strand at 1441.629 kb on + strand at 1441.629 kb on + strand at 1441.655 kb on - strand at 1441.684 kb on + strand at 1441.712 kb on + strand at 1441.843 kb on + strand at 1441.844 kb on - strand at 1441.854 kb on + strand at 1441.855 kb on - strand at 1441.911 kb on + strand, within HER17_RS06470 at 1441.912 kb on - strand, within HER17_RS06470 at 1441.912 kb on - strand, within HER17_RS06470 at 1441.971 kb on + strand, within HER17_RS06470 at 1441.972 kb on - strand, within HER17_RS06470 at 1442.162 kb on + strand, within HER17_RS06470 at 1442.165 kb on + strand, within HER17_RS06470 at 1442.166 kb on - strand, within HER17_RS06470 at 1442.166 kb on - strand, within HER17_RS06470 at 1442.215 kb on + strand, within HER17_RS06470 at 1442.232 kb on + strand, within HER17_RS06470 at 1442.232 kb on + strand, within HER17_RS06470 at 1442.532 kb on + strand, within HER17_RS06470 at 1442.532 kb on + strand, within HER17_RS06470 at 1442.533 kb on - strand, within HER17_RS06470 at 1442.745 kb on - strand, within HER17_RS06470 at 1442.851 kb on - strand at 1442.853 kb on + strand at 1442.854 kb on - strand at 1443.063 kb on + strand, within HER17_RS06475 at 1443.063 kb on + strand, within HER17_RS06475 at 1443.063 kb on + strand, within HER17_RS06475 at 1443.224 kb on + strand, within HER17_RS06475 at 1443.224 kb on + strand, within HER17_RS06475 at 1443.224 kb on + strand, within HER17_RS06475 at 1443.225 kb on - strand, within HER17_RS06475 at 1443.399 kb on + strand at 1443.400 kb on - strand at 1443.463 kb on + strand at 1443.463 kb on + strand at 1443.463 kb on + strand at 1443.464 kb on - strand at 1443.464 kb on - strand at 1443.475 kb on - strand at 1443.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+starch remove 1,440,829 + HER17_RS06465 0.65 +1.2 1,440,829 + HER17_RS06465 0.65 +0.4 1,440,829 + HER17_RS06465 0.65 +0.5 1,440,830 - HER17_RS06465 0.65 -0.1 1,440,830 - HER17_RS06465 0.65 +0.7 1,440,852 - HER17_RS06465 0.67 -0.0 1,440,910 + HER17_RS06465 0.71 -0.6 1,440,911 - HER17_RS06465 0.71 -0.4 1,440,914 + HER17_RS06465 0.71 +1.4 1,440,915 - HER17_RS06465 0.71 +1.1 1,440,938 - HER17_RS06465 0.73 -0.4 1,441,089 + HER17_RS06465 0.85 -0.7 1,441,092 + HER17_RS06465 0.85 +0.2 1,441,092 + HER17_RS06465 0.85 -0.4 1,441,092 + HER17_RS06465 0.85 -0.2 1,441,092 + HER17_RS06465 0.85 -0.2 1,441,093 - HER17_RS06465 0.85 +0.1 1,441,093 - HER17_RS06465 0.85 +0.1 1,441,124 - HER17_RS06465 0.88 +0.7 1,441,163 + -0.5 1,441,169 + +0.6 1,441,170 - +0.3 1,441,228 + -0.1 1,441,282 + -0.1 1,441,282 + -0.3 1,441,283 - -0.1 1,441,284 + +1.0 1,441,284 + +0.8 1,441,308 - +0.3 1,441,308 - -1.2 1,441,310 + +2.4 1,441,311 - +0.6 1,441,414 - -0.1 1,441,414 - +0.2 1,441,433 + -0.8 1,441,454 + -0.6 1,441,454 + -1.0 1,441,455 - -0.4 1,441,455 - -0.4 1,441,455 - -1.0 1,441,455 - -0.8 1,441,555 + -1.2 1,441,629 + -0.7 1,441,629 + -0.6 1,441,655 - +0.4 1,441,684 + -2.9 1,441,712 + -0.3 1,441,843 + -2.3 1,441,844 - -1.2 1,441,854 + -1.8 1,441,855 - -3.2 1,441,911 + HER17_RS06470 0.10 -1.2 1,441,912 - HER17_RS06470 0.10 -2.4 1,441,912 - HER17_RS06470 0.10 -2.8 1,441,971 + HER17_RS06470 0.16 -2.0 1,441,972 - HER17_RS06470 0.16 -3.0 1,442,162 + HER17_RS06470 0.34 -1.9 1,442,165 + HER17_RS06470 0.34 -2.3 1,442,166 - HER17_RS06470 0.34 -4.0 1,442,166 - HER17_RS06470 0.34 -1.3 1,442,215 + HER17_RS06470 0.39 -2.9 1,442,232 + HER17_RS06470 0.40 -3.2 1,442,232 + HER17_RS06470 0.40 -2.0 1,442,532 + HER17_RS06470 0.68 -2.9 1,442,532 + HER17_RS06470 0.68 -0.8 1,442,533 - HER17_RS06470 0.68 -2.2 1,442,745 - HER17_RS06470 0.88 -2.5 1,442,851 - -3.2 1,442,853 + -1.2 1,442,854 - -2.4 1,443,063 + HER17_RS06475 0.38 -1.9 1,443,063 + HER17_RS06475 0.38 -2.4 1,443,063 + HER17_RS06475 0.38 -3.2 1,443,224 + HER17_RS06475 0.70 -2.8 1,443,224 + HER17_RS06475 0.70 -0.9 1,443,224 + HER17_RS06475 0.70 -1.7 1,443,225 - HER17_RS06475 0.70 -2.6 1,443,399 + -2.1 1,443,400 - -1.2 1,443,463 + -2.6 1,443,463 + -3.4 1,443,463 + -1.9 1,443,464 - -2.3 1,443,464 - -0.6 1,443,475 - -0.4 1,443,475 - -2.5
Or see this region's nucleotide sequence