Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS12030 and HER17_RS12035 are separated by 97 nucleotides HER17_RS12035 and HER17_RS12040 are separated by 86 nucleotides HER17_RS12040 and HER17_RS12045 are separated by 178 nucleotides
HER17_RS12030: HER17_RS12030 - aspartate/tyrosine/aromatic aminotransferase, at 2,677,375 to 2,678,565
_RS12030
HER17_RS12035: HER17_RS12035 - MBL fold metallo-hydrolase, at 2,678,663 to 2,679,310
_RS12035
HER17_RS12040: HER17_RS12040 - YcbK family protein, at 2,679,397 to 2,679,945
_RS12040
HER17_RS12045: HER17_RS12045 - L,D-transpeptidase, at 2,680,124 to 2,681,863
_RS12045
Position (kb)
2678
2679
2680 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2677.777 kb on - strand, within HER17_RS12030 at 2677.822 kb on + strand, within HER17_RS12030 at 2678.061 kb on + strand, within HER17_RS12030 at 2678.093 kb on + strand, within HER17_RS12030 at 2678.094 kb on - strand, within HER17_RS12030 at 2678.104 kb on + strand, within HER17_RS12030 at 2678.221 kb on + strand, within HER17_RS12030 at 2678.221 kb on + strand, within HER17_RS12030 at 2678.221 kb on + strand, within HER17_RS12030 at 2678.335 kb on - strand, within HER17_RS12030 at 2678.355 kb on + strand, within HER17_RS12030 at 2678.356 kb on - strand, within HER17_RS12030 at 2678.648 kb on + strand at 2678.704 kb on + strand at 2678.705 kb on - strand at 2678.717 kb on + strand at 2678.780 kb on - strand, within HER17_RS12035 at 2678.855 kb on - strand, within HER17_RS12035 at 2678.914 kb on + strand, within HER17_RS12035 at 2678.914 kb on + strand, within HER17_RS12035 at 2678.915 kb on - strand, within HER17_RS12035 at 2678.915 kb on - strand, within HER17_RS12035 at 2679.042 kb on - strand, within HER17_RS12035 at 2679.257 kb on + strand at 2679.257 kb on + strand at 2679.258 kb on - strand at 2679.303 kb on + strand at 2679.328 kb on - strand at 2679.449 kb on + strand at 2679.452 kb on + strand, within HER17_RS12040 at 2679.452 kb on + strand, within HER17_RS12040 at 2679.453 kb on - strand, within HER17_RS12040 at 2679.453 kb on - strand, within HER17_RS12040 at 2679.587 kb on - strand, within HER17_RS12040 at 2679.600 kb on + strand, within HER17_RS12040 at 2679.600 kb on + strand, within HER17_RS12040 at 2679.601 kb on - strand, within HER17_RS12040 at 2679.601 kb on - strand, within HER17_RS12040 at 2679.601 kb on - strand, within HER17_RS12040 at 2679.605 kb on - strand, within HER17_RS12040 at 2679.605 kb on - strand, within HER17_RS12040 at 2679.632 kb on - strand, within HER17_RS12040 at 2679.708 kb on + strand, within HER17_RS12040 at 2679.762 kb on + strand, within HER17_RS12040 at 2679.763 kb on - strand, within HER17_RS12040 at 2679.763 kb on - strand, within HER17_RS12040 at 2679.945 kb on + strand at 2679.954 kb on + strand at 2679.976 kb on - strand at 2680.009 kb on + strand at 2680.091 kb on + strand at 2680.092 kb on - strand at 2680.092 kb on - strand at 2680.112 kb on + strand at 2680.122 kb on - strand at 2680.125 kb on + strand at 2680.125 kb on + strand at 2680.126 kb on - strand at 2680.126 kb on - strand at 2680.185 kb on + strand at 2680.185 kb on + strand at 2680.186 kb on - strand at 2680.191 kb on + strand at 2680.242 kb on - strand at 2680.245 kb on + strand at 2680.245 kb on + strand at 2680.249 kb on - strand at 2680.249 kb on - strand at 2680.249 kb on - strand at 2680.249 kb on - strand at 2680.249 kb on - strand at 2680.249 kb on - strand at 2680.291 kb on + strand at 2680.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 2,677,777 - HER17_RS12030 0.34 +0.3 2,677,822 + HER17_RS12030 0.38 -1.2 2,678,061 + HER17_RS12030 0.58 -1.4 2,678,093 + HER17_RS12030 0.60 -1.7 2,678,094 - HER17_RS12030 0.60 -0.4 2,678,104 + HER17_RS12030 0.61 -1.2 2,678,221 + HER17_RS12030 0.71 -0.3 2,678,221 + HER17_RS12030 0.71 -1.8 2,678,221 + HER17_RS12030 0.71 -1.4 2,678,335 - HER17_RS12030 0.81 -2.4 2,678,355 + HER17_RS12030 0.82 +0.5 2,678,356 - HER17_RS12030 0.82 +0.1 2,678,648 + -1.2 2,678,704 + -0.1 2,678,705 - +1.8 2,678,717 + +0.3 2,678,780 - HER17_RS12035 0.18 -0.8 2,678,855 - HER17_RS12035 0.30 -0.6 2,678,914 + HER17_RS12035 0.39 -0.6 2,678,914 + HER17_RS12035 0.39 +1.2 2,678,915 - HER17_RS12035 0.39 +0.6 2,678,915 - HER17_RS12035 0.39 -0.2 2,679,042 - HER17_RS12035 0.58 +0.1 2,679,257 + -1.1 2,679,257 + +1.2 2,679,258 - -2.0 2,679,303 + -0.2 2,679,328 - -1.0 2,679,449 + -1.4 2,679,452 + HER17_RS12040 0.10 +0.5 2,679,452 + HER17_RS12040 0.10 -0.3 2,679,453 - HER17_RS12040 0.10 +0.1 2,679,453 - HER17_RS12040 0.10 +0.1 2,679,587 - HER17_RS12040 0.35 -0.1 2,679,600 + HER17_RS12040 0.37 -0.2 2,679,600 + HER17_RS12040 0.37 +0.1 2,679,601 - HER17_RS12040 0.37 -1.5 2,679,601 - HER17_RS12040 0.37 +0.2 2,679,601 - HER17_RS12040 0.37 -0.6 2,679,605 - HER17_RS12040 0.38 +0.7 2,679,605 - HER17_RS12040 0.38 -0.7 2,679,632 - HER17_RS12040 0.43 -1.9 2,679,708 + HER17_RS12040 0.57 +1.1 2,679,762 + HER17_RS12040 0.66 +0.9 2,679,763 - HER17_RS12040 0.67 +0.3 2,679,763 - HER17_RS12040 0.67 +1.1 2,679,945 + +0.1 2,679,954 + +0.1 2,679,976 - -0.0 2,680,009 + +0.4 2,680,091 + +0.3 2,680,092 - +0.8 2,680,092 - -0.5 2,680,112 + -1.2 2,680,122 - +0.4 2,680,125 + -0.4 2,680,125 + -0.3 2,680,126 - +0.1 2,680,126 - -1.2 2,680,185 + -2.1 2,680,185 + +0.1 2,680,186 - -0.5 2,680,191 + +0.7 2,680,242 - -1.5 2,680,245 + +0.8 2,680,245 + -0.1 2,680,249 - +0.7 2,680,249 - -0.7 2,680,249 - +0.3 2,680,249 - +0.4 2,680,249 - -1.7 2,680,249 - +0.3 2,680,291 + -1.3 2,680,292 - +0.0
Or see this region's nucleotide sequence