Strain Fitness in Pectobacterium carotovorum WPP14 around HER17_RS09975

Experiment: M9+sucrose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHER17_RS09965 and HER17_RS09970 are separated by 10 nucleotidesHER17_RS09970 and HER17_RS09975 are separated by 6 nucleotidesHER17_RS09975 and HER17_RS09980 are separated by 7 nucleotidesHER17_RS09980 and HER17_RS09985 overlap by 4 nucleotides HER17_RS09965: HER17_RS09965 - type III secretion system export apparatus subunit SctU, at 2,204,928 to 2,206,007 _RS09965 HER17_RS09970: HER17_RS09970 - type III secretion system export apparatus subunit SctT, at 2,206,018 to 2,206,821 _RS09970 HER17_RS09975: HER17_RS09975 - type III secretion system export apparatus subunit SctS, at 2,206,828 to 2,207,088 _RS09975 HER17_RS09980: HER17_RS09980 - type III secretion system export apparatus subunit SctR, at 2,207,096 to 2,207,749 _RS09980 HER17_RS09985: HER17_RS09985 - type III secretion system cytoplasmic ring protein SctQ, at 2,207,746 to 2,208,900 _RS09985 Position (kb) 2206 2207 2208Strain fitness (log2 ratio) -2 -1 0 1 2at 2205.840 kb on + strand, within HER17_RS09965at 2205.841 kb on - strand, within HER17_RS09965at 2205.841 kb on - strand, within HER17_RS09965at 2205.886 kb on - strand, within HER17_RS09965at 2206.039 kb on - strandat 2206.096 kb on + strandat 2206.101 kb on - strand, within HER17_RS09970at 2206.191 kb on + strand, within HER17_RS09970at 2206.191 kb on + strand, within HER17_RS09970at 2206.204 kb on + strand, within HER17_RS09970at 2206.298 kb on + strand, within HER17_RS09970at 2206.299 kb on - strand, within HER17_RS09970at 2206.359 kb on - strand, within HER17_RS09970at 2206.584 kb on + strand, within HER17_RS09970at 2206.743 kb on + strandat 2206.776 kb on + strandat 2206.777 kb on - strandat 2206.831 kb on + strandat 2206.832 kb on - strandat 2206.832 kb on - strandat 2206.886 kb on - strand, within HER17_RS09975at 2206.985 kb on - strand, within HER17_RS09975at 2207.080 kb on + strandat 2207.081 kb on - strandat 2207.103 kb on + strandat 2207.103 kb on + strandat 2207.104 kb on - strandat 2207.104 kb on - strandat 2207.107 kb on + strandat 2207.107 kb on + strandat 2207.107 kb on + strandat 2207.107 kb on + strandat 2207.108 kb on - strandat 2207.109 kb on + strandat 2207.109 kb on + strandat 2207.109 kb on + strandat 2207.109 kb on + strandat 2207.109 kb on + strandat 2207.110 kb on - strandat 2207.110 kb on - strandat 2207.110 kb on - strandat 2207.110 kb on - strandat 2207.168 kb on + strand, within HER17_RS09980at 2207.262 kb on + strand, within HER17_RS09980at 2207.262 kb on + strand, within HER17_RS09980at 2207.262 kb on + strand, within HER17_RS09980at 2207.262 kb on + strand, within HER17_RS09980at 2207.263 kb on - strand, within HER17_RS09980at 2207.345 kb on + strand, within HER17_RS09980at 2207.346 kb on - strand, within HER17_RS09980at 2207.393 kb on + strand, within HER17_RS09980at 2207.393 kb on + strand, within HER17_RS09980at 2207.394 kb on - strand, within HER17_RS09980at 2207.394 kb on - strand, within HER17_RS09980at 2207.394 kb on - strand, within HER17_RS09980at 2207.431 kb on + strand, within HER17_RS09980at 2207.432 kb on - strand, within HER17_RS09980at 2207.774 kb on + strandat 2207.775 kb on - strandat 2207.834 kb on - strandat 2207.894 kb on + strand, within HER17_RS09985at 2207.894 kb on + strand, within HER17_RS09985at 2207.894 kb on + strand, within HER17_RS09985at 2207.895 kb on - strand, within HER17_RS09985at 2208.001 kb on + strand, within HER17_RS09985at 2208.002 kb on - strand, within HER17_RS09985at 2208.002 kb on - strand, within HER17_RS09985at 2208.057 kb on - strand, within HER17_RS09985

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Per-strain Table

Position Strand Gene LocusTag Fraction M9+sucrose
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2,205,840 + HER17_RS09965 0.84 -1.9
2,205,841 - HER17_RS09965 0.85 -0.0
2,205,841 - HER17_RS09965 0.85 +0.6
2,205,886 - HER17_RS09965 0.89 -0.2
2,206,039 - -0.8
2,206,096 + -0.9
2,206,101 - HER17_RS09970 0.10 -0.3
2,206,191 + HER17_RS09970 0.22 +1.0
2,206,191 + HER17_RS09970 0.22 -0.3
2,206,204 + HER17_RS09970 0.23 +0.2
2,206,298 + HER17_RS09970 0.35 -2.2
2,206,299 - HER17_RS09970 0.35 +1.3
2,206,359 - HER17_RS09970 0.42 -0.4
2,206,584 + HER17_RS09970 0.70 -1.1
2,206,743 + +1.2
2,206,776 + +0.4
2,206,777 - -1.0
2,206,831 + +0.4
2,206,832 - +0.8
2,206,832 - +0.7
2,206,886 - HER17_RS09975 0.22 -0.5
2,206,985 - HER17_RS09975 0.60 +0.4
2,207,080 + +0.7
2,207,081 - -0.4
2,207,103 + -0.1
2,207,103 + -0.9
2,207,104 - +1.0
2,207,104 - -0.0
2,207,107 + +0.6
2,207,107 + +2.5
2,207,107 + -0.7
2,207,107 + +0.8
2,207,108 - +1.0
2,207,109 + -0.0
2,207,109 + +1.0
2,207,109 + +0.4
2,207,109 + -0.1
2,207,109 + +0.7
2,207,110 - -0.4
2,207,110 - -0.3
2,207,110 - -1.1
2,207,110 - +0.3
2,207,168 + HER17_RS09980 0.11 +1.0
2,207,262 + HER17_RS09980 0.25 -0.3
2,207,262 + HER17_RS09980 0.25 +1.1
2,207,262 + HER17_RS09980 0.25 +0.4
2,207,262 + HER17_RS09980 0.25 +0.2
2,207,263 - HER17_RS09980 0.26 -0.7
2,207,345 + HER17_RS09980 0.38 +0.2
2,207,346 - HER17_RS09980 0.38 -0.4
2,207,393 + HER17_RS09980 0.45 -0.5
2,207,393 + HER17_RS09980 0.45 +0.4
2,207,394 - HER17_RS09980 0.46 +2.0
2,207,394 - HER17_RS09980 0.46 -0.3
2,207,394 - HER17_RS09980 0.46 -0.9
2,207,431 + HER17_RS09980 0.51 +0.9
2,207,432 - HER17_RS09980 0.51 -0.2
2,207,774 + -1.8
2,207,775 - -0.2
2,207,834 - -0.5
2,207,894 + HER17_RS09985 0.13 -0.6
2,207,894 + HER17_RS09985 0.13 -0.4
2,207,894 + HER17_RS09985 0.13 +0.2
2,207,895 - HER17_RS09985 0.13 -2.2
2,208,001 + HER17_RS09985 0.22 +0.4
2,208,002 - HER17_RS09985 0.22 +1.2
2,208,002 - HER17_RS09985 0.22 +0.6
2,208,057 - HER17_RS09985 0.27 +0.3

Or see this region's nucleotide sequence