Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS06640 and HER17_RS06645 are separated by 80 nucleotides HER17_RS06645 and HER17_RS06650 are separated by 42 nucleotides
HER17_RS06640: HER17_RS06640 - DUF3772 domain-containing protein, at 1,476,082 to 1,478,535
_RS06640
HER17_RS06645: HER17_RS06645 - D-amino-acid transaminase, at 1,478,616 to 1,479,476
_RS06645
HER17_RS06650: HER17_RS06650 - L-Ala-D/L-Glu epimerase, at 1,479,519 to 1,480,499
_RS06650
Position (kb)
1478
1479
1480 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1477.631 kb on + strand, within HER17_RS06640 at 1477.631 kb on + strand, within HER17_RS06640 at 1477.775 kb on - strand, within HER17_RS06640 at 1477.775 kb on - strand, within HER17_RS06640 at 1477.781 kb on + strand, within HER17_RS06640 at 1477.782 kb on - strand, within HER17_RS06640 at 1477.782 kb on - strand, within HER17_RS06640 at 1477.821 kb on + strand, within HER17_RS06640 at 1477.833 kb on - strand, within HER17_RS06640 at 1478.005 kb on - strand, within HER17_RS06640 at 1478.061 kb on + strand, within HER17_RS06640 at 1478.062 kb on - strand, within HER17_RS06640 at 1478.094 kb on + strand, within HER17_RS06640 at 1478.182 kb on - strand, within HER17_RS06640 at 1478.182 kb on - strand, within HER17_RS06640 at 1478.184 kb on + strand, within HER17_RS06640 at 1478.185 kb on - strand, within HER17_RS06640 at 1478.278 kb on + strand, within HER17_RS06640 at 1478.278 kb on + strand, within HER17_RS06640 at 1478.278 kb on + strand, within HER17_RS06640 at 1478.279 kb on - strand, within HER17_RS06640 at 1478.279 kb on - strand, within HER17_RS06640 at 1478.279 kb on - strand, within HER17_RS06640 at 1478.372 kb on - strand at 1478.530 kb on - strand at 1478.533 kb on + strand at 1478.534 kb on - strand at 1478.534 kb on - strand at 1478.534 kb on - strand at 1478.534 kb on - strand at 1478.534 kb on - strand at 1478.618 kb on - strand at 1478.633 kb on + strand at 1478.633 kb on + strand at 1478.647 kb on + strand at 1478.771 kb on - strand, within HER17_RS06645 at 1478.849 kb on + strand, within HER17_RS06645 at 1478.864 kb on + strand, within HER17_RS06645 at 1478.864 kb on + strand, within HER17_RS06645 at 1478.864 kb on + strand, within HER17_RS06645 at 1478.887 kb on - strand, within HER17_RS06645 at 1478.887 kb on - strand, within HER17_RS06645 at 1478.914 kb on + strand, within HER17_RS06645 at 1478.914 kb on + strand, within HER17_RS06645 at 1478.914 kb on + strand, within HER17_RS06645 at 1478.915 kb on - strand, within HER17_RS06645 at 1478.915 kb on - strand, within HER17_RS06645 at 1478.992 kb on + strand, within HER17_RS06645 at 1478.993 kb on - strand, within HER17_RS06645 at 1478.993 kb on - strand, within HER17_RS06645 at 1478.993 kb on - strand, within HER17_RS06645 at 1479.129 kb on + strand, within HER17_RS06645 at 1479.197 kb on + strand, within HER17_RS06645 at 1479.204 kb on + strand, within HER17_RS06645 at 1479.205 kb on - strand, within HER17_RS06645 at 1479.208 kb on + strand, within HER17_RS06645 at 1479.209 kb on - strand, within HER17_RS06645 at 1479.297 kb on + strand, within HER17_RS06645 at 1479.379 kb on + strand, within HER17_RS06645 at 1479.379 kb on + strand, within HER17_RS06645 at 1479.379 kb on + strand, within HER17_RS06645 at 1479.380 kb on - strand, within HER17_RS06645 at 1479.380 kb on - strand, within HER17_RS06645 at 1479.414 kb on + strand at 1479.442 kb on - strand at 1479.445 kb on + strand at 1479.446 kb on - strand at 1479.446 kb on - strand at 1479.446 kb on - strand at 1479.490 kb on - strand at 1479.490 kb on - strand at 1479.490 kb on - strand at 1479.492 kb on + strand at 1479.492 kb on + strand at 1479.493 kb on - strand at 1479.531 kb on + strand at 1479.531 kb on + strand at 1479.531 kb on + strand at 1479.532 kb on - strand at 1479.549 kb on + strand at 1479.563 kb on + strand at 1479.565 kb on + strand at 1479.565 kb on + strand at 1479.565 kb on + strand at 1479.565 kb on + strand at 1479.565 kb on + strand at 1479.566 kb on - strand at 1479.566 kb on - strand at 1479.566 kb on - strand at 1479.671 kb on + strand, within HER17_RS06650 at 1479.671 kb on + strand, within HER17_RS06650 at 1479.671 kb on + strand, within HER17_RS06650 at 1479.672 kb on - strand, within HER17_RS06650 at 1479.692 kb on + strand, within HER17_RS06650 at 1479.718 kb on + strand, within HER17_RS06650 at 1479.718 kb on + strand, within HER17_RS06650 at 1479.719 kb on - strand, within HER17_RS06650 at 1479.759 kb on - strand, within HER17_RS06650 at 1479.759 kb on - strand, within HER17_RS06650 at 1479.996 kb on + strand, within HER17_RS06650 at 1480.130 kb on + strand, within HER17_RS06650 at 1480.131 kb on - strand, within HER17_RS06650 at 1480.131 kb on - strand, within HER17_RS06650 at 1480.133 kb on - strand, within HER17_RS06650 at 1480.152 kb on - strand, within HER17_RS06650 at 1480.281 kb on + strand, within HER17_RS06650 at 1480.282 kb on - strand, within HER17_RS06650 at 1480.282 kb on - strand, within HER17_RS06650 at 1480.327 kb on - strand, within HER17_RS06650 at 1480.327 kb on - strand, within HER17_RS06650 at 1480.335 kb on + strand, within HER17_RS06650 at 1480.336 kb on - strand, within HER17_RS06650 at 1480.336 kb on - strand, within HER17_RS06650 at 1480.357 kb on + strand, within HER17_RS06650 at 1480.461 kb on + strand at 1480.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 1,477,631 + HER17_RS06640 0.63 -0.8 1,477,631 + HER17_RS06640 0.63 -0.7 1,477,775 - HER17_RS06640 0.69 +0.4 1,477,775 - HER17_RS06640 0.69 +2.1 1,477,781 + HER17_RS06640 0.69 +0.2 1,477,782 - HER17_RS06640 0.69 +0.2 1,477,782 - HER17_RS06640 0.69 -0.6 1,477,821 + HER17_RS06640 0.71 -0.4 1,477,833 - HER17_RS06640 0.71 -0.5 1,478,005 - HER17_RS06640 0.78 +0.6 1,478,061 + HER17_RS06640 0.81 +0.3 1,478,062 - HER17_RS06640 0.81 +0.5 1,478,094 + HER17_RS06640 0.82 -1.0 1,478,182 - HER17_RS06640 0.86 -0.5 1,478,182 - HER17_RS06640 0.86 +0.5 1,478,184 + HER17_RS06640 0.86 +0.1 1,478,185 - HER17_RS06640 0.86 +0.5 1,478,278 + HER17_RS06640 0.89 -2.5 1,478,278 + HER17_RS06640 0.89 -0.4 1,478,278 + HER17_RS06640 0.89 -0.0 1,478,279 - HER17_RS06640 0.90 -0.6 1,478,279 - HER17_RS06640 0.90 +0.4 1,478,279 - HER17_RS06640 0.90 +0.1 1,478,372 - +1.0 1,478,530 - +0.4 1,478,533 + -0.6 1,478,534 - +0.6 1,478,534 - -0.1 1,478,534 - +0.9 1,478,534 - -1.1 1,478,534 - -0.6 1,478,618 - +0.9 1,478,633 + +1.8 1,478,633 + +0.1 1,478,647 + +2.1 1,478,771 - HER17_RS06645 0.18 -0.0 1,478,849 + HER17_RS06645 0.27 +0.2 1,478,864 + HER17_RS06645 0.29 +0.3 1,478,864 + HER17_RS06645 0.29 -0.6 1,478,864 + HER17_RS06645 0.29 -0.4 1,478,887 - HER17_RS06645 0.31 +0.4 1,478,887 - HER17_RS06645 0.31 +1.4 1,478,914 + HER17_RS06645 0.35 -0.2 1,478,914 + HER17_RS06645 0.35 -0.0 1,478,914 + HER17_RS06645 0.35 -1.2 1,478,915 - HER17_RS06645 0.35 -2.1 1,478,915 - HER17_RS06645 0.35 +0.3 1,478,992 + HER17_RS06645 0.44 +0.1 1,478,993 - HER17_RS06645 0.44 +0.7 1,478,993 - HER17_RS06645 0.44 -0.8 1,478,993 - HER17_RS06645 0.44 +0.3 1,479,129 + HER17_RS06645 0.60 -0.3 1,479,197 + HER17_RS06645 0.67 -0.4 1,479,204 + HER17_RS06645 0.68 -1.6 1,479,205 - HER17_RS06645 0.68 -0.4 1,479,208 + HER17_RS06645 0.69 +0.2 1,479,209 - HER17_RS06645 0.69 +0.2 1,479,297 + HER17_RS06645 0.79 +0.1 1,479,379 + HER17_RS06645 0.89 +0.9 1,479,379 + HER17_RS06645 0.89 -0.2 1,479,379 + HER17_RS06645 0.89 +0.1 1,479,380 - HER17_RS06645 0.89 +0.6 1,479,380 - HER17_RS06645 0.89 -0.6 1,479,414 + -0.7 1,479,442 - +0.9 1,479,445 + -0.2 1,479,446 - -0.4 1,479,446 - -0.1 1,479,446 - -2.3 1,479,490 - +2.0 1,479,490 - +0.0 1,479,490 - -0.4 1,479,492 + +0.8 1,479,492 + +0.4 1,479,493 - -0.5 1,479,531 + +0.0 1,479,531 + +0.2 1,479,531 + -0.9 1,479,532 - -0.4 1,479,549 + +0.0 1,479,563 + -0.8 1,479,565 + +0.3 1,479,565 + -0.6 1,479,565 + -0.3 1,479,565 + +0.5 1,479,565 + -0.2 1,479,566 - +0.3 1,479,566 - -0.2 1,479,566 - -0.0 1,479,671 + HER17_RS06650 0.15 -0.2 1,479,671 + HER17_RS06650 0.15 +0.0 1,479,671 + HER17_RS06650 0.15 +0.1 1,479,672 - HER17_RS06650 0.16 +1.1 1,479,692 + HER17_RS06650 0.18 -0.3 1,479,718 + HER17_RS06650 0.20 -0.6 1,479,718 + HER17_RS06650 0.20 -0.2 1,479,719 - HER17_RS06650 0.20 +1.1 1,479,759 - HER17_RS06650 0.24 +0.9 1,479,759 - HER17_RS06650 0.24 +1.4 1,479,996 + HER17_RS06650 0.49 +0.2 1,480,130 + HER17_RS06650 0.62 -1.5 1,480,131 - HER17_RS06650 0.62 -1.2 1,480,131 - HER17_RS06650 0.62 +0.3 1,480,133 - HER17_RS06650 0.63 +0.2 1,480,152 - HER17_RS06650 0.65 -0.2 1,480,281 + HER17_RS06650 0.78 -0.5 1,480,282 - HER17_RS06650 0.78 -1.6 1,480,282 - HER17_RS06650 0.78 -3.0 1,480,327 - HER17_RS06650 0.82 -0.7 1,480,327 - HER17_RS06650 0.82 -0.3 1,480,335 + HER17_RS06650 0.83 +1.0 1,480,336 - HER17_RS06650 0.83 +0.2 1,480,336 - HER17_RS06650 0.83 +1.6 1,480,357 + HER17_RS06650 0.85 +0.2 1,480,461 + +0.5 1,480,462 - +0.5
Or see this region's nucleotide sequence