Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS01470 and HER17_RS01475 are separated by 155 nucleotides HER17_RS01475 and HER17_RS01480 are separated by 40 nucleotides
HER17_RS01470: HER17_RS01470 - aspartate-semialdehyde dehydrogenase, at 336,877 to 337,980
_RS01470
HER17_RS01475: HER17_RS01475 - hypothetical protein, at 338,136 to 338,627
_RS01475
HER17_RS01480: HER17_RS01480 - sensor histidine kinase, at 338,668 to 340,386
_RS01480
Position (kb) 338
339 Strain fitness (log2 ratio) -2
-1
0
1
2 at 337.978 kb on + strand at 337.978 kb on + strand at 337.978 kb on + strand at 337.978 kb on + strand at 337.978 kb on + strand at 337.979 kb on - strand at 337.979 kb on - strand at 337.979 kb on - strand at 337.979 kb on - strand at 337.979 kb on - strand at 337.979 kb on - strand at 337.982 kb on + strand at 337.983 kb on - strand at 337.983 kb on - strand at 337.983 kb on - strand at 337.983 kb on - strand at 337.990 kb on + strand at 337.990 kb on + strand at 337.990 kb on + strand at 337.990 kb on + strand at 337.991 kb on - strand at 337.991 kb on - strand at 337.992 kb on + strand at 337.992 kb on + strand at 337.992 kb on + strand at 337.993 kb on - strand at 337.993 kb on - strand at 337.993 kb on - strand at 337.993 kb on - strand at 338.042 kb on + strand at 338.042 kb on + strand at 338.043 kb on - strand at 338.043 kb on - strand at 338.048 kb on + strand at 338.048 kb on + strand at 338.049 kb on - strand at 338.049 kb on - strand at 338.049 kb on - strand at 338.049 kb on - strand at 338.049 kb on - strand at 338.062 kb on + strand at 338.062 kb on + strand at 338.062 kb on + strand at 338.062 kb on + strand at 338.062 kb on + strand at 338.063 kb on - strand at 338.063 kb on - strand at 338.063 kb on - strand at 338.063 kb on - strand at 338.093 kb on + strand at 338.094 kb on - strand at 338.094 kb on - strand at 338.094 kb on - strand at 338.236 kb on + strand, within HER17_RS01475 at 338.236 kb on + strand, within HER17_RS01475 at 338.237 kb on - strand, within HER17_RS01475 at 338.256 kb on + strand, within HER17_RS01475 at 338.257 kb on - strand, within HER17_RS01475 at 338.292 kb on + strand, within HER17_RS01475 at 338.292 kb on + strand, within HER17_RS01475 at 338.292 kb on + strand, within HER17_RS01475 at 338.293 kb on - strand, within HER17_RS01475 at 338.293 kb on - strand, within HER17_RS01475 at 338.293 kb on - strand, within HER17_RS01475 at 338.335 kb on + strand, within HER17_RS01475 at 338.397 kb on + strand, within HER17_RS01475 at 338.398 kb on - strand, within HER17_RS01475 at 338.398 kb on - strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.399 kb on + strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.400 kb on - strand, within HER17_RS01475 at 338.404 kb on - strand, within HER17_RS01475 at 338.423 kb on + strand, within HER17_RS01475 at 338.424 kb on - strand, within HER17_RS01475 at 338.424 kb on - strand, within HER17_RS01475 at 338.424 kb on - strand, within HER17_RS01475 at 338.476 kb on + strand, within HER17_RS01475 at 338.476 kb on + strand, within HER17_RS01475 at 338.477 kb on - strand, within HER17_RS01475 at 338.500 kb on - strand, within HER17_RS01475 at 338.515 kb on + strand, within HER17_RS01475 at 338.516 kb on - strand, within HER17_RS01475 at 338.516 kb on - strand, within HER17_RS01475 at 338.525 kb on + strand, within HER17_RS01475 at 338.525 kb on + strand, within HER17_RS01475 at 338.526 kb on - strand, within HER17_RS01475 at 338.527 kb on + strand, within HER17_RS01475 at 338.608 kb on + strand at 338.640 kb on + strand at 338.640 kb on + strand at 338.640 kb on + strand at 338.641 kb on - strand at 338.641 kb on - strand at 338.641 kb on - strand at 338.643 kb on + strand at 338.646 kb on + strand at 338.646 kb on + strand at 338.646 kb on + strand at 338.646 kb on + strand at 338.646 kb on + strand at 338.646 kb on + strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.647 kb on - strand at 338.653 kb on + strand at 338.653 kb on + strand at 338.653 kb on + strand at 338.653 kb on + strand at 338.654 kb on - strand at 338.654 kb on - strand at 338.680 kb on - strand at 338.696 kb on - strand at 338.725 kb on + strand at 338.725 kb on + strand at 338.852 kb on + strand, within HER17_RS01480 at 338.852 kb on + strand, within HER17_RS01480 at 338.872 kb on + strand, within HER17_RS01480 at 338.873 kb on - strand, within HER17_RS01480 at 338.879 kb on - strand, within HER17_RS01480 at 338.947 kb on - strand, within HER17_RS01480 at 339.105 kb on + strand, within HER17_RS01480 at 339.199 kb on + strand, within HER17_RS01480 at 339.199 kb on + strand, within HER17_RS01480 at 339.216 kb on + strand, within HER17_RS01480 at 339.216 kb on + strand, within HER17_RS01480 at 339.228 kb on + strand, within HER17_RS01480 at 339.229 kb on - strand, within HER17_RS01480 at 339.361 kb on - strand, within HER17_RS01480 at 339.379 kb on + strand, within HER17_RS01480 at 339.385 kb on - strand, within HER17_RS01480 at 339.469 kb on - strand, within HER17_RS01480 at 339.469 kb on - strand, within HER17_RS01480 at 339.492 kb on + strand, within HER17_RS01480 at 339.492 kb on + strand, within HER17_RS01480 at 339.492 kb on + strand, within HER17_RS01480 at 339.492 kb on + strand, within HER17_RS01480 at 339.494 kb on + strand, within HER17_RS01480 at 339.494 kb on + strand, within HER17_RS01480 at 339.494 kb on + strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.495 kb on - strand, within HER17_RS01480 at 339.518 kb on + strand, within HER17_RS01480 at 339.519 kb on - strand, within HER17_RS01480 at 339.567 kb on + strand, within HER17_RS01480
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 337,978 + +0.4 337,978 + -0.1 337,978 + +0.3 337,978 + +0.6 337,978 + -0.2 337,979 - -0.7 337,979 - +0.3 337,979 - -0.9 337,979 - +1.2 337,979 - -0.7 337,979 - +0.0 337,982 + +0.6 337,983 - +0.6 337,983 - +0.9 337,983 - -0.2 337,983 - -0.1 337,990 + -1.9 337,990 + +0.5 337,990 + +0.7 337,990 + -0.2 337,991 - +0.6 337,991 - +2.0 337,992 + +0.6 337,992 + -0.3 337,992 + +1.6 337,993 - +0.9 337,993 - -0.6 337,993 - +0.9 337,993 - +0.3 338,042 + -1.0 338,042 + +0.2 338,043 - -0.5 338,043 - -1.1 338,048 + +1.2 338,048 + +0.5 338,049 - -0.3 338,049 - -0.7 338,049 - -0.1 338,049 - -0.0 338,049 - -1.6 338,062 + -1.1 338,062 + +0.2 338,062 + -0.4 338,062 + -0.8 338,062 + +0.3 338,063 - +0.6 338,063 - -0.3 338,063 - -0.9 338,063 - -1.1 338,093 + -0.2 338,094 - +0.2 338,094 - +1.0 338,094 - -1.2 338,236 + HER17_RS01475 0.20 +1.5 338,236 + HER17_RS01475 0.20 +0.0 338,237 - HER17_RS01475 0.21 -0.5 338,256 + HER17_RS01475 0.24 +0.3 338,257 - HER17_RS01475 0.25 +1.3 338,292 + HER17_RS01475 0.32 -0.3 338,292 + HER17_RS01475 0.32 -2.0 338,292 + HER17_RS01475 0.32 -0.8 338,293 - HER17_RS01475 0.32 +0.0 338,293 - HER17_RS01475 0.32 +0.4 338,293 - HER17_RS01475 0.32 -0.3 338,335 + HER17_RS01475 0.40 -2.1 338,397 + HER17_RS01475 0.53 -1.8 338,398 - HER17_RS01475 0.53 +0.5 338,398 - HER17_RS01475 0.53 +0.2 338,399 + HER17_RS01475 0.53 -0.1 338,399 + HER17_RS01475 0.53 -1.3 338,399 + HER17_RS01475 0.53 +0.1 338,399 + HER17_RS01475 0.53 -0.3 338,399 + HER17_RS01475 0.53 +0.1 338,399 + HER17_RS01475 0.53 +1.6 338,399 + HER17_RS01475 0.53 +0.5 338,400 - HER17_RS01475 0.54 -0.8 338,400 - HER17_RS01475 0.54 -1.1 338,400 - HER17_RS01475 0.54 -1.0 338,400 - HER17_RS01475 0.54 +0.4 338,400 - HER17_RS01475 0.54 -0.7 338,400 - HER17_RS01475 0.54 -0.6 338,404 - HER17_RS01475 0.54 -0.9 338,423 + HER17_RS01475 0.58 +0.2 338,424 - HER17_RS01475 0.59 -0.2 338,424 - HER17_RS01475 0.59 +1.1 338,424 - HER17_RS01475 0.59 +1.5 338,476 + HER17_RS01475 0.69 -0.6 338,476 + HER17_RS01475 0.69 -0.4 338,477 - HER17_RS01475 0.69 -0.2 338,500 - HER17_RS01475 0.74 +0.5 338,515 + HER17_RS01475 0.77 +0.2 338,516 - HER17_RS01475 0.77 -0.3 338,516 - HER17_RS01475 0.77 +0.2 338,525 + HER17_RS01475 0.79 -1.1 338,525 + HER17_RS01475 0.79 -0.4 338,526 - HER17_RS01475 0.79 -0.2 338,527 + HER17_RS01475 0.79 +1.0 338,608 + +0.7 338,640 + +0.0 338,640 + -0.2 338,640 + -0.5 338,641 - +0.8 338,641 - +0.3 338,641 - +0.0 338,643 + +0.3 338,646 + -0.5 338,646 + -0.2 338,646 + -2.4 338,646 + +0.2 338,646 + -0.3 338,646 + -0.1 338,647 - -1.3 338,647 - +0.4 338,647 - -0.5 338,653 + -0.6 338,653 + +0.4 338,653 + -0.5 338,653 + -0.5 338,654 - +0.6 338,654 - -0.1 338,680 - +1.3 338,696 - -0.5 338,725 + -0.6 338,725 + -0.3 338,852 + HER17_RS01480 0.11 +0.7 338,852 + HER17_RS01480 0.11 -0.4 338,872 + HER17_RS01480 0.12 -0.4 338,873 - HER17_RS01480 0.12 -0.2 338,879 - HER17_RS01480 0.12 -0.4 338,947 - HER17_RS01480 0.16 -0.0 339,105 + HER17_RS01480 0.25 +0.6 339,199 + HER17_RS01480 0.31 +1.1 339,199 + HER17_RS01480 0.31 -1.7 339,216 + HER17_RS01480 0.32 -0.0 339,216 + HER17_RS01480 0.32 +1.0 339,228 + HER17_RS01480 0.33 +0.4 339,229 - HER17_RS01480 0.33 -0.3 339,361 - HER17_RS01480 0.40 -0.5 339,379 + HER17_RS01480 0.41 -0.9 339,385 - HER17_RS01480 0.42 -0.6 339,469 - HER17_RS01480 0.47 -0.0 339,469 - HER17_RS01480 0.47 -2.0 339,492 + HER17_RS01480 0.48 -0.8 339,492 + HER17_RS01480 0.48 +1.2 339,492 + HER17_RS01480 0.48 +0.0 339,492 + HER17_RS01480 0.48 +0.0 339,494 + HER17_RS01480 0.48 -0.5 339,494 + HER17_RS01480 0.48 -1.1 339,494 + HER17_RS01480 0.48 -1.4 339,495 - HER17_RS01480 0.48 -2.5 339,495 - HER17_RS01480 0.48 +1.6 339,495 - HER17_RS01480 0.48 -0.2 339,495 - HER17_RS01480 0.48 +0.1 339,495 - HER17_RS01480 0.48 -0.7 339,495 - HER17_RS01480 0.48 +1.7 339,495 - HER17_RS01480 0.48 -2.3 339,495 - HER17_RS01480 0.48 -0.2 339,495 - HER17_RS01480 0.48 -0.2 339,518 + HER17_RS01480 0.49 +1.0 339,519 - HER17_RS01480 0.50 -0.3 339,567 + HER17_RS01480 0.52 +0.1
Or see this region's nucleotide sequence