Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15455 and HER17_RS15460 are separated by 328 nucleotides HER17_RS15460 and HER17_RS15465 are separated by 337 nucleotides
HER17_RS15455: HER17_RS15455 - uracil permease, at 3,463,897 to 3,465,186
_RS15455
HER17_RS15460: HER17_RS15460 - uracil phosphoribosyltransferase, at 3,465,515 to 3,466,141
_RS15460
HER17_RS15465: HER17_RS15465 - phosphoribosylformylglycinamidine cyclo-ligase, at 3,466,479 to 3,467,516
_RS15465
Position (kb)
3465
3466
3467 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3464.529 kb on + strand, within HER17_RS15455 at 3464.529 kb on + strand, within HER17_RS15455 at 3464.529 kb on + strand, within HER17_RS15455 at 3464.530 kb on - strand, within HER17_RS15455 at 3464.530 kb on - strand, within HER17_RS15455 at 3464.531 kb on + strand, within HER17_RS15455 at 3464.532 kb on - strand, within HER17_RS15455 at 3464.532 kb on - strand, within HER17_RS15455 at 3464.532 kb on - strand, within HER17_RS15455 at 3464.610 kb on - strand, within HER17_RS15455 at 3464.610 kb on - strand, within HER17_RS15455 at 3464.862 kb on + strand, within HER17_RS15455 at 3464.901 kb on + strand, within HER17_RS15455 at 3464.902 kb on - strand, within HER17_RS15455 at 3464.904 kb on + strand, within HER17_RS15455 at 3464.904 kb on + strand, within HER17_RS15455 at 3464.905 kb on - strand, within HER17_RS15455 at 3464.905 kb on - strand, within HER17_RS15455 at 3464.905 kb on - strand, within HER17_RS15455 at 3464.927 kb on + strand, within HER17_RS15455 at 3464.985 kb on - strand, within HER17_RS15455 at 3464.985 kb on - strand, within HER17_RS15455 at 3465.013 kb on + strand, within HER17_RS15455 at 3465.017 kb on + strand, within HER17_RS15455 at 3465.018 kb on - strand, within HER17_RS15455 at 3465.018 kb on - strand, within HER17_RS15455 at 3465.050 kb on - strand, within HER17_RS15455 at 3465.142 kb on + strand at 3465.143 kb on - strand at 3465.143 kb on - strand at 3465.202 kb on + strand at 3465.202 kb on + strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.203 kb on - strand at 3465.211 kb on + strand at 3465.211 kb on + strand at 3465.211 kb on + strand at 3465.211 kb on + strand at 3465.211 kb on + strand at 3465.212 kb on - strand at 3465.212 kb on - strand at 3465.215 kb on + strand at 3465.215 kb on + strand at 3465.215 kb on + strand at 3465.216 kb on - strand at 3465.216 kb on - strand at 3465.216 kb on - strand at 3465.216 kb on - strand at 3465.216 kb on - strand at 3465.228 kb on - strand at 3465.243 kb on - strand at 3465.291 kb on + strand at 3465.291 kb on + strand at 3465.291 kb on + strand at 3465.291 kb on + strand at 3465.291 kb on + strand at 3465.292 kb on - strand at 3465.292 kb on - strand at 3465.295 kb on - strand at 3465.295 kb on - strand at 3465.295 kb on - strand at 3465.335 kb on + strand at 3465.354 kb on - strand at 3465.358 kb on - strand at 3465.429 kb on + strand at 3465.460 kb on + strand at 3465.477 kb on - strand at 3465.477 kb on - strand at 3465.497 kb on - strand at 3465.510 kb on - strand at 3465.510 kb on - strand at 3465.510 kb on - strand at 3465.516 kb on + strand at 3465.568 kb on - strand at 3465.568 kb on - strand at 3465.606 kb on + strand, within HER17_RS15460 at 3465.678 kb on + strand, within HER17_RS15460 at 3465.805 kb on - strand, within HER17_RS15460 at 3465.805 kb on - strand, within HER17_RS15460 at 3465.805 kb on - strand, within HER17_RS15460 at 3465.838 kb on - strand, within HER17_RS15460 at 3465.838 kb on - strand, within HER17_RS15460 at 3466.020 kb on + strand, within HER17_RS15460 at 3466.020 kb on + strand, within HER17_RS15460 at 3466.069 kb on + strand, within HER17_RS15460 at 3466.069 kb on + strand, within HER17_RS15460 at 3466.070 kb on - strand, within HER17_RS15460 at 3466.072 kb on + strand, within HER17_RS15460 at 3466.073 kb on - strand, within HER17_RS15460 at 3466.073 kb on - strand, within HER17_RS15460 at 3466.073 kb on - strand, within HER17_RS15460 at 3466.076 kb on - strand, within HER17_RS15460 at 3466.097 kb on + strand at 3466.097 kb on + strand at 3466.097 kb on + strand at 3466.098 kb on - strand at 3466.145 kb on + strand at 3466.145 kb on + strand at 3466.146 kb on - strand at 3466.260 kb on + strand at 3466.261 kb on - strand at 3466.263 kb on - strand at 3466.279 kb on - strand at 3466.279 kb on - strand at 3466.292 kb on + strand at 3466.292 kb on + strand at 3466.382 kb on - strand at 3466.396 kb on - strand at 3466.405 kb on + strand at 3466.419 kb on + strand at 3466.419 kb on + strand at 3466.419 kb on + strand at 3466.419 kb on + strand at 3466.420 kb on - strand at 3466.420 kb on - strand at 3466.449 kb on - strand at 3466.503 kb on + strand at 3466.504 kb on - strand at 3466.547 kb on - strand at 3466.553 kb on + strand at 3466.553 kb on + strand at 3466.564 kb on + strand at 3466.564 kb on + strand at 3466.565 kb on - strand at 3466.592 kb on + strand, within HER17_RS15465 at 3466.592 kb on + strand, within HER17_RS15465 at 3466.924 kb on - strand, within HER17_RS15465 at 3466.924 kb on - strand, within HER17_RS15465 at 3466.924 kb on - strand, within HER17_RS15465 at 3466.924 kb on - strand, within HER17_RS15465 at 3466.924 kb on - strand, within HER17_RS15465 at 3467.123 kb on + strand, within HER17_RS15465
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 3,464,529 + HER17_RS15455 0.49 +0.3 3,464,529 + HER17_RS15455 0.49 -0.2 3,464,529 + HER17_RS15455 0.49 +0.5 3,464,530 - HER17_RS15455 0.49 -1.9 3,464,530 - HER17_RS15455 0.49 -0.3 3,464,531 + HER17_RS15455 0.49 +0.9 3,464,532 - HER17_RS15455 0.49 -0.1 3,464,532 - HER17_RS15455 0.49 -1.3 3,464,532 - HER17_RS15455 0.49 -0.7 3,464,610 - HER17_RS15455 0.55 -0.4 3,464,610 - HER17_RS15455 0.55 -0.0 3,464,862 + HER17_RS15455 0.75 -0.9 3,464,901 + HER17_RS15455 0.78 -0.8 3,464,902 - HER17_RS15455 0.78 -0.4 3,464,904 + HER17_RS15455 0.78 +1.8 3,464,904 + HER17_RS15455 0.78 +0.2 3,464,905 - HER17_RS15455 0.78 +0.4 3,464,905 - HER17_RS15455 0.78 -1.2 3,464,905 - HER17_RS15455 0.78 -0.4 3,464,927 + HER17_RS15455 0.80 -1.1 3,464,985 - HER17_RS15455 0.84 -1.2 3,464,985 - HER17_RS15455 0.84 +0.2 3,465,013 + HER17_RS15455 0.87 +1.0 3,465,017 + HER17_RS15455 0.87 +1.1 3,465,018 - HER17_RS15455 0.87 +0.9 3,465,018 - HER17_RS15455 0.87 -0.1 3,465,050 - HER17_RS15455 0.89 -0.3 3,465,142 + -0.6 3,465,143 - +0.2 3,465,143 - +1.0 3,465,202 + -0.0 3,465,202 + +0.3 3,465,203 - -0.3 3,465,203 - -0.3 3,465,203 - -1.9 3,465,203 - +1.0 3,465,211 + -0.6 3,465,211 + -0.3 3,465,211 + -1.3 3,465,211 + +0.1 3,465,211 + -0.6 3,465,212 - +0.8 3,465,212 - +0.2 3,465,215 + +0.9 3,465,215 + -0.1 3,465,215 + -0.2 3,465,216 - +1.0 3,465,216 - +0.1 3,465,216 - +0.0 3,465,216 - +0.5 3,465,216 - +0.0 3,465,228 - -0.4 3,465,243 - +0.4 3,465,291 + +0.7 3,465,291 + +1.0 3,465,291 + +0.4 3,465,291 + +1.2 3,465,291 + +0.0 3,465,292 - +0.6 3,465,292 - -0.4 3,465,295 - -0.3 3,465,295 - +0.3 3,465,295 - -0.0 3,465,335 + +0.6 3,465,354 - -0.1 3,465,358 - +0.4 3,465,429 + -0.6 3,465,460 + -0.1 3,465,477 - -0.4 3,465,477 - -0.6 3,465,497 - -0.4 3,465,510 - +0.3 3,465,510 - -0.5 3,465,510 - -0.1 3,465,516 + -0.3 3,465,568 - -1.5 3,465,568 - -2.4 3,465,606 + HER17_RS15460 0.15 -1.4 3,465,678 + HER17_RS15460 0.26 -0.1 3,465,805 - HER17_RS15460 0.46 +0.0 3,465,805 - HER17_RS15460 0.46 -1.9 3,465,805 - HER17_RS15460 0.46 +1.4 3,465,838 - HER17_RS15460 0.52 -0.0 3,465,838 - HER17_RS15460 0.52 +0.4 3,466,020 + HER17_RS15460 0.81 +0.6 3,466,020 + HER17_RS15460 0.81 -1.7 3,466,069 + HER17_RS15460 0.88 -1.2 3,466,069 + HER17_RS15460 0.88 +0.6 3,466,070 - HER17_RS15460 0.89 +1.0 3,466,072 + HER17_RS15460 0.89 -0.5 3,466,073 - HER17_RS15460 0.89 -1.1 3,466,073 - HER17_RS15460 0.89 -0.6 3,466,073 - HER17_RS15460 0.89 +0.3 3,466,076 - HER17_RS15460 0.89 -0.9 3,466,097 + +0.1 3,466,097 + +0.3 3,466,097 + +0.0 3,466,098 - -0.2 3,466,145 + +1.1 3,466,145 + -0.1 3,466,146 - +0.7 3,466,260 + -1.3 3,466,261 - +0.3 3,466,263 - +1.4 3,466,279 - +0.0 3,466,279 - -0.5 3,466,292 + +0.2 3,466,292 + -0.2 3,466,382 - -1.9 3,466,396 - -1.2 3,466,405 + -1.8 3,466,419 + +0.1 3,466,419 + +0.2 3,466,419 + -3.0 3,466,419 + -0.9 3,466,420 - -2.1 3,466,420 - -1.2 3,466,449 - -2.5 3,466,503 + -2.4 3,466,504 - -3.3 3,466,547 - -1.2 3,466,553 + -2.4 3,466,553 + -1.8 3,466,564 + -2.5 3,466,564 + -3.1 3,466,565 - -4.9 3,466,592 + HER17_RS15465 0.11 -1.9 3,466,592 + HER17_RS15465 0.11 -1.2 3,466,924 - HER17_RS15465 0.43 -0.6 3,466,924 - HER17_RS15465 0.43 -4.3 3,466,924 - HER17_RS15465 0.43 -1.3 3,466,924 - HER17_RS15465 0.43 -0.6 3,466,924 - HER17_RS15465 0.43 -3.0 3,467,123 + HER17_RS15465 0.62 -2.7
Or see this region's nucleotide sequence