Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS02870 and HER17_RS02875 overlap by 4 nucleotides HER17_RS02875 and HER17_RS02880 overlap by 1 nucleotides HER17_RS02880 and HER17_RS02885 overlap by 20 nucleotides HER17_RS02885 and HER17_RS02890 are separated by 337 nucleotides
HER17_RS02870: HER17_RS02870 - isoleucine--tRNA ligase, at 644,679 to 647,492
_RS02870
HER17_RS02875: HER17_RS02875 - signal peptidase II, at 647,489 to 648,004
_RS02875
HER17_RS02880: HER17_RS02880 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 648,004 to 648,477
_RS02880
HER17_RS02885: HER17_RS02885 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 648,458 to 649,408
_RS02885
HER17_RS02890: HER17_RS02890 - 4-hydroxy-tetrahydrodipicolinate reductase, at 649,746 to 650,567
_RS02890
Position (kb) 648
649
650 Strain fitness (log2 ratio) -2
-1
0
1 at 647.974 kb on + strand at 648.024 kb on + strand at 648.024 kb on + strand at 648.291 kb on + strand, within HER17_RS02880 at 648.297 kb on + strand, within HER17_RS02880 at 648.297 kb on + strand, within HER17_RS02880 at 648.327 kb on + strand, within HER17_RS02880 at 649.402 kb on - strand at 649.407 kb on - strand at 649.407 kb on - strand at 649.471 kb on + strand at 649.497 kb on + strand at 649.562 kb on + strand at 649.563 kb on - strand at 649.563 kb on - strand at 649.563 kb on - strand at 649.563 kb on - strand at 649.563 kb on - strand at 649.648 kb on - strand at 649.708 kb on + strand at 649.709 kb on - strand at 649.709 kb on - strand at 649.709 kb on - strand at 649.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 647,974 + -0.8 648,024 + +0.8 648,024 + +0.4 648,291 + HER17_RS02880 0.61 +0.2 648,297 + HER17_RS02880 0.62 +0.2 648,297 + HER17_RS02880 0.62 -1.9 648,327 + HER17_RS02880 0.68 -1.0 649,402 - -0.8 649,407 - +0.7 649,407 - +0.4 649,471 + -0.5 649,497 + +0.5 649,562 + +0.2 649,563 - -1.6 649,563 - -0.6 649,563 - -0.0 649,563 - -0.4 649,563 - -1.0 649,648 - -0.5 649,708 + +0.3 649,709 - -1.6 649,709 - -2.6 649,709 - -1.5 649,745 + +0.1
Or see this region's nucleotide sequence