Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15935 and HER17_RS15940 are separated by 68 nucleotides HER17_RS15940 and HER17_RS15945 overlap by 26 nucleotides HER17_RS15945 and HER17_RS15950 are separated by 68 nucleotides HER17_RS15950 and HER17_RS15955 are separated by 24 nucleotides
HER17_RS15935: HER17_RS15935 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,578,973 to 3,580,838
_RS15935
HER17_RS15940: HER17_RS15940 - phosphatidylglycerophosphatase A, at 3,580,907 to 3,581,443
_RS15940
HER17_RS15945: HER17_RS15945 - thiamine-phosphate kinase, at 3,581,418 to 3,582,398
_RS15945
HER17_RS15950: HER17_RS15950 - transcription antitermination factor NusB, at 3,582,467 to 3,582,886
_RS15950
HER17_RS15955: HER17_RS15955 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,582,911 to 3,583,387
_RS15955
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-1
0
1 at 3580.837 kb on - strand at 3580.841 kb on + strand at 3580.842 kb on - strand at 3580.842 kb on - strand at 3580.842 kb on - strand at 3580.878 kb on - strand at 3580.939 kb on - strand at 3580.942 kb on + strand at 3580.943 kb on - strand at 3580.984 kb on + strand, within HER17_RS15940 at 3580.985 kb on - strand, within HER17_RS15940 at 3581.093 kb on - strand, within HER17_RS15940 at 3581.200 kb on - strand, within HER17_RS15940 at 3581.210 kb on - strand, within HER17_RS15940 at 3581.433 kb on - strand at 3581.443 kb on + strand at 3581.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 3,580,837 - +0.5 3,580,841 + +0.4 3,580,842 - -0.7 3,580,842 - -0.6 3,580,842 - +0.2 3,580,878 - -0.6 3,580,939 - -0.7 3,580,942 + +1.0 3,580,943 - +1.2 3,580,984 + HER17_RS15940 0.14 -0.4 3,580,985 - HER17_RS15940 0.15 +1.0 3,581,093 - HER17_RS15940 0.35 +1.2 3,581,200 - HER17_RS15940 0.55 -1.0 3,581,210 - HER17_RS15940 0.56 +0.2 3,581,433 - +0.0 3,581,443 + +1.2 3,581,444 - -0.4
Or see this region's nucleotide sequence