Experiment: M9+sucrose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS09520 and HER17_RS09525 are separated by 90 nucleotides HER17_RS09525 and HER17_RS09530 overlap by 1 nucleotides HER17_RS09530 and HER17_RS09535 are separated by 92 nucleotides HER17_RS09535 and HER17_RS09540 are separated by 144 nucleotides HER17_RS09540 and HER17_RS09545 are separated by 12 nucleotides
HER17_RS09520: HER17_RS09520 - phage shock protein PspA, at 2,094,016 to 2,094,681
_RS09520
HER17_RS09525: HER17_RS09525 - envelope stress response membrane protein PspB, at 2,094,772 to 2,094,996
_RS09525
HER17_RS09530: HER17_RS09530 - envelope stress response membrane protein PspC, at 2,094,996 to 2,095,361
_RS09530
HER17_RS09535: HER17_RS09535 - hypothetical protein, at 2,095,454 to 2,096,008
_RS09535
HER17_RS09540: HER17_RS09540 - hypothetical protein, at 2,096,153 to 2,096,635
_RS09540
HER17_RS09545: HER17_RS09545 - hemagglutinin repeat-containing protein, at 2,096,648 to 2,104,453
_RS09545
Position (kb)
2095
2096
2097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2094.470 kb on + strand, within HER17_RS09520 at 2094.654 kb on + strand at 2094.654 kb on + strand at 2094.655 kb on - strand at 2094.655 kb on - strand at 2094.666 kb on + strand at 2094.690 kb on - strand at 2094.753 kb on + strand at 2094.753 kb on + strand at 2094.754 kb on - strand at 2094.760 kb on + strand at 2094.761 kb on - strand at 2094.761 kb on - strand at 2094.830 kb on + strand, within HER17_RS09525 at 2094.842 kb on + strand, within HER17_RS09525 at 2094.847 kb on + strand, within HER17_RS09525 at 2094.848 kb on - strand, within HER17_RS09525 at 2094.858 kb on + strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.891 kb on - strand, within HER17_RS09525 at 2094.928 kb on - strand, within HER17_RS09525 at 2095.004 kb on + strand at 2095.005 kb on - strand at 2095.027 kb on - strand at 2095.047 kb on - strand, within HER17_RS09530 at 2095.116 kb on - strand, within HER17_RS09530 at 2095.164 kb on + strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.165 kb on - strand, within HER17_RS09530 at 2095.181 kb on + strand, within HER17_RS09530 at 2095.182 kb on - strand, within HER17_RS09530 at 2095.235 kb on + strand, within HER17_RS09530 at 2095.252 kb on + strand, within HER17_RS09530 at 2095.252 kb on + strand, within HER17_RS09530 at 2095.311 kb on + strand, within HER17_RS09530 at 2095.312 kb on - strand, within HER17_RS09530 at 2095.312 kb on - strand, within HER17_RS09530 at 2095.326 kb on - strand at 2095.360 kb on - strand at 2095.360 kb on - strand at 2095.377 kb on - strand at 2095.455 kb on + strand at 2095.529 kb on + strand, within HER17_RS09535 at 2095.530 kb on - strand, within HER17_RS09535 at 2095.657 kb on + strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.658 kb on - strand, within HER17_RS09535 at 2095.660 kb on + strand, within HER17_RS09535 at 2095.671 kb on + strand, within HER17_RS09535 at 2095.671 kb on + strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535 at 2095.672 kb on - strand, within HER17_RS09535 at 2095.687 kb on - strand, within HER17_RS09535 at 2095.687 kb on - strand, within HER17_RS09535 at 2095.687 kb on - strand, within HER17_RS09535 at 2095.696 kb on + strand, within HER17_RS09535 at 2095.696 kb on + strand, within HER17_RS09535 at 2095.734 kb on + strand, within HER17_RS09535 at 2095.737 kb on + strand, within HER17_RS09535 at 2095.738 kb on - strand, within HER17_RS09535 at 2095.738 kb on - strand, within HER17_RS09535 at 2095.749 kb on + strand, within HER17_RS09535 at 2095.750 kb on - strand, within HER17_RS09535 at 2095.796 kb on + strand, within HER17_RS09535 at 2095.796 kb on + strand, within HER17_RS09535 at 2095.797 kb on - strand, within HER17_RS09535 at 2095.797 kb on - strand, within HER17_RS09535 at 2095.799 kb on + strand, within HER17_RS09535 at 2095.799 kb on + strand, within HER17_RS09535 at 2095.854 kb on + strand, within HER17_RS09535 at 2095.880 kb on + strand, within HER17_RS09535 at 2095.880 kb on + strand, within HER17_RS09535 at 2095.881 kb on - strand, within HER17_RS09535 at 2095.881 kb on - strand, within HER17_RS09535 at 2095.885 kb on + strand, within HER17_RS09535 at 2095.889 kb on + strand, within HER17_RS09535 at 2095.973 kb on + strand at 2095.987 kb on + strand at 2096.038 kb on - strand at 2096.066 kb on + strand at 2096.080 kb on + strand at 2096.112 kb on + strand at 2096.130 kb on - strand at 2096.138 kb on + strand at 2096.144 kb on - strand at 2096.159 kb on - strand at 2096.628 kb on - strand at 2096.770 kb on + strand at 2096.788 kb on + strand at 2096.789 kb on - strand at 2096.883 kb on - strand at 2096.897 kb on - strand at 2096.897 kb on - strand at 2096.904 kb on + strand at 2096.904 kb on + strand at 2096.904 kb on + strand at 2096.904 kb on + strand at 2096.905 kb on - strand at 2096.905 kb on - strand at 2096.905 kb on - strand at 2096.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+sucrose remove 2,094,470 + HER17_RS09520 0.68 +0.1 2,094,654 + +1.2 2,094,654 + -0.3 2,094,655 - -1.5 2,094,655 - +0.2 2,094,666 + +0.8 2,094,690 - -0.8 2,094,753 + +0.2 2,094,753 + -0.1 2,094,754 - +1.0 2,094,760 + -0.2 2,094,761 - +0.5 2,094,761 - +0.7 2,094,830 + HER17_RS09525 0.26 +1.7 2,094,842 + HER17_RS09525 0.31 +0.4 2,094,847 + HER17_RS09525 0.33 +0.8 2,094,848 - HER17_RS09525 0.34 -0.0 2,094,858 + HER17_RS09525 0.38 +0.2 2,094,891 - HER17_RS09525 0.53 +1.3 2,094,891 - HER17_RS09525 0.53 +0.4 2,094,891 - HER17_RS09525 0.53 -0.9 2,094,891 - HER17_RS09525 0.53 +0.2 2,094,928 - HER17_RS09525 0.69 -0.6 2,095,004 + -2.2 2,095,005 - -1.0 2,095,027 - -0.2 2,095,047 - HER17_RS09530 0.14 -1.0 2,095,116 - HER17_RS09530 0.33 +2.2 2,095,164 + HER17_RS09530 0.46 -2.8 2,095,165 - HER17_RS09530 0.46 +0.7 2,095,165 - HER17_RS09530 0.46 -2.4 2,095,165 - HER17_RS09530 0.46 -1.2 2,095,181 + HER17_RS09530 0.51 +0.8 2,095,182 - HER17_RS09530 0.51 -0.1 2,095,235 + HER17_RS09530 0.65 -0.0 2,095,252 + HER17_RS09530 0.70 +3.4 2,095,252 + HER17_RS09530 0.70 +0.9 2,095,311 + HER17_RS09530 0.86 +0.4 2,095,312 - HER17_RS09530 0.86 +0.1 2,095,312 - HER17_RS09530 0.86 -0.6 2,095,326 - +0.2 2,095,360 - +0.4 2,095,360 - +1.3 2,095,377 - -0.0 2,095,455 + +0.5 2,095,529 + HER17_RS09535 0.14 -1.1 2,095,530 - HER17_RS09535 0.14 -0.3 2,095,657 + HER17_RS09535 0.37 -0.5 2,095,658 - HER17_RS09535 0.37 -0.1 2,095,658 - HER17_RS09535 0.37 -0.2 2,095,658 - HER17_RS09535 0.37 -1.3 2,095,658 - HER17_RS09535 0.37 +0.2 2,095,660 + HER17_RS09535 0.37 -0.5 2,095,671 + HER17_RS09535 0.39 -0.8 2,095,671 + HER17_RS09535 0.39 -0.4 2,095,672 - HER17_RS09535 0.39 -0.4 2,095,672 - HER17_RS09535 0.39 -0.5 2,095,672 - HER17_RS09535 0.39 +0.2 2,095,687 - HER17_RS09535 0.42 +1.1 2,095,687 - HER17_RS09535 0.42 -0.9 2,095,687 - HER17_RS09535 0.42 +0.9 2,095,696 + HER17_RS09535 0.44 -1.3 2,095,696 + HER17_RS09535 0.44 +0.7 2,095,734 + HER17_RS09535 0.50 +0.9 2,095,737 + HER17_RS09535 0.51 -0.2 2,095,738 - HER17_RS09535 0.51 +0.5 2,095,738 - HER17_RS09535 0.51 +0.1 2,095,749 + HER17_RS09535 0.53 +0.8 2,095,750 - HER17_RS09535 0.53 -0.4 2,095,796 + HER17_RS09535 0.62 -0.1 2,095,796 + HER17_RS09535 0.62 +0.4 2,095,797 - HER17_RS09535 0.62 +0.8 2,095,797 - HER17_RS09535 0.62 -0.8 2,095,799 + HER17_RS09535 0.62 -0.3 2,095,799 + HER17_RS09535 0.62 +0.2 2,095,854 + HER17_RS09535 0.72 -0.4 2,095,880 + HER17_RS09535 0.77 +0.5 2,095,880 + HER17_RS09535 0.77 -0.2 2,095,881 - HER17_RS09535 0.77 -1.2 2,095,881 - HER17_RS09535 0.77 -0.6 2,095,885 + HER17_RS09535 0.78 -2.2 2,095,889 + HER17_RS09535 0.78 -0.2 2,095,973 + +0.2 2,095,987 + -0.9 2,096,038 - -0.1 2,096,066 + +1.1 2,096,080 + +0.3 2,096,112 + +1.2 2,096,130 - +0.5 2,096,138 + -0.8 2,096,144 - -1.7 2,096,159 - -0.3 2,096,628 - +0.0 2,096,770 + +0.4 2,096,788 + -0.4 2,096,789 - +1.1 2,096,883 - -0.9 2,096,897 - +0.1 2,096,897 - +1.2 2,096,904 + +0.0 2,096,904 + +0.6 2,096,904 + +0.2 2,096,904 + -0.0 2,096,905 - -0.4 2,096,905 - -0.0 2,096,905 - -1.8 2,096,910 - +1.1
Or see this region's nucleotide sequence