Experiment: M9+glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HER17_RS15235 and HER17_RS15240 are separated by 1 nucleotides HER17_RS15240 and HER17_RS15245 are separated by 33 nucleotides HER17_RS15245 and HER17_RS15250 are separated by 9 nucleotides
HER17_RS15235: HER17_RS15235 - ribosome silencing factor, at 3,418,231 to 3,418,548
_RS15235
HER17_RS15240: HER17_RS15240 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 3,418,550 to 3,419,020
_RS15240
HER17_RS15245: HER17_RS15245 - peptidoglycan DD-transpeptidase MrdA, at 3,419,054 to 3,420,958
_RS15245
HER17_RS15250: HER17_RS15250 - peptidoglycan glycosyltransferase MrdB, at 3,420,968 to 3,422,080
_RS15250
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3418.069 kb on + strand at 3418.069 kb on + strand at 3418.070 kb on - strand at 3418.070 kb on - strand at 3418.071 kb on + strand at 3418.094 kb on + strand at 3418.094 kb on + strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.097 kb on - strand at 3418.266 kb on + strand, within HER17_RS15235 at 3418.266 kb on + strand, within HER17_RS15235 at 3418.266 kb on + strand, within HER17_RS15235 at 3418.280 kb on + strand, within HER17_RS15235 at 3418.280 kb on + strand, within HER17_RS15235 at 3418.305 kb on + strand, within HER17_RS15235 at 3418.336 kb on + strand, within HER17_RS15235 at 3418.336 kb on + strand, within HER17_RS15235 at 3418.344 kb on + strand, within HER17_RS15235 at 3418.362 kb on + strand, within HER17_RS15235 at 3418.380 kb on + strand, within HER17_RS15235 at 3418.475 kb on + strand, within HER17_RS15235 at 3418.522 kb on + strand at 3418.529 kb on + strand at 3418.549 kb on + strand at 3418.762 kb on + strand, within HER17_RS15240 at 3418.815 kb on + strand, within HER17_RS15240 at 3419.026 kb on + strand at 3420.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glucose remove 3,418,069 + +0.7 3,418,069 + -3.4 3,418,070 - -1.0 3,418,070 - -1.1 3,418,071 + -2.5 3,418,094 + +0.7 3,418,094 + +1.2 3,418,097 - +1.7 3,418,097 - +1.5 3,418,097 - +2.2 3,418,097 - +2.5 3,418,266 + HER17_RS15235 0.11 -0.7 3,418,266 + HER17_RS15235 0.11 -2.2 3,418,266 + HER17_RS15235 0.11 +0.5 3,418,280 + HER17_RS15235 0.15 +0.2 3,418,280 + HER17_RS15235 0.15 -0.5 3,418,305 + HER17_RS15235 0.23 +0.7 3,418,336 + HER17_RS15235 0.33 +0.3 3,418,336 + HER17_RS15235 0.33 +1.3 3,418,344 + HER17_RS15235 0.36 +1.7 3,418,362 + HER17_RS15235 0.41 +1.0 3,418,380 + HER17_RS15235 0.47 +1.1 3,418,475 + HER17_RS15235 0.77 +1.1 3,418,522 + +0.3 3,418,529 + -0.6 3,418,549 + -0.8 3,418,762 + HER17_RS15240 0.45 -2.3 3,418,815 + HER17_RS15240 0.56 -0.2 3,419,026 + +0.4 3,420,915 + +0.9
Or see this region's nucleotide sequence