Experiment: M9+glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS05505 and DDA3937_RS05510 are separated by 120 nucleotides DDA3937_RS05510 and DDA3937_RS05515 are separated by 22 nucleotides DDA3937_RS05515 and DDA3937_RS05520 are separated by 60 nucleotides
DDA3937_RS05505: DDA3937_RS05505 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 1,231,715 to 1,232,827
_RS05505
DDA3937_RS05510: DDA3937_RS05510 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,232,948 to 1,233,421
_RS05510
DDA3937_RS05515: DDA3937_RS05515 - transcription antitermination factor NusB, at 1,233,444 to 1,233,863
_RS05515
DDA3937_RS05520: DDA3937_RS05520 - thiamine-phosphate kinase, at 1,233,924 to 1,234,898
_RS05520
Position (kb)
1233
1234 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1232.825 kb on + strand at 1232.825 kb on + strand at 1232.826 kb on - strand at 1232.826 kb on - strand at 1232.826 kb on - strand at 1232.828 kb on + strand at 1232.828 kb on + strand at 1232.829 kb on - strand at 1232.829 kb on - strand at 1232.829 kb on - strand at 1232.829 kb on - strand at 1232.897 kb on + strand at 1232.897 kb on + strand at 1232.897 kb on + strand at 1232.897 kb on + strand at 1232.902 kb on + strand at 1232.927 kb on + strand at 1233.711 kb on + strand, within DDA3937_RS05515 at 1233.923 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9+glucose remove 1,232,825 + +1.1 1,232,825 + -0.7 1,232,826 - -0.5 1,232,826 - +0.5 1,232,826 - +0.5 1,232,828 + +0.5 1,232,828 + -2.1 1,232,829 - +0.9 1,232,829 - -0.4 1,232,829 - -0.5 1,232,829 - -1.9 1,232,897 + +0.3 1,232,897 + -0.7 1,232,897 + -0.5 1,232,897 + -0.7 1,232,902 + -1.7 1,232,927 + -1.6 1,233,711 + DDA3937_RS05515 0.64 -2.4 1,233,923 + +2.1
Or see this region's nucleotide sequence