Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0061 and PP_0062 are separated by 83 nucleotides PP_0062 and PP_0063 are separated by 41 nucleotides PP_0063 and PP_0064 are separated by 97 nucleotides PP_0064 and PP_0065 are separated by 11 nucleotides
PP_0061: PP_0061 - glycine-tRNA ligase alpha subunit, at 71,901 to 72,848
_0061
PP_0062: PP_0062 - 3-methyl-adenine DNA glycosylase I, at 72,932 to 73,483
_0062
PP_0063: PP_0063 - putative Lipid A biosynthesis lauroyl acyltransferase, at 73,525 to 74,412
_0063
PP_0064: PP_0064 - conserved protein of unknown function, at 74,510 to 74,824
_0064
PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209
_0065
Position (kb) 73
74
75 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 72.893 kb on - strand at 72.893 kb on - strand at 72.893 kb on - strand at 72.895 kb on - strand at 72.917 kb on - strand at 72.932 kb on - strand at 72.932 kb on - strand at 72.969 kb on - strand at 73.073 kb on - strand, within PP_0062 at 73.073 kb on - strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.128 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.407 kb on - strand, within PP_0062 at 73.489 kb on + strand at 73.489 kb on + strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.512 kb on - strand at 73.662 kb on - strand, within PP_0063 at 73.746 kb on + strand at 73.747 kb on - strand, within PP_0063 at 73.858 kb on - strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.916 kb on - strand, within PP_0063 at 74.082 kb on + strand, within PP_0063 at 74.195 kb on + strand, within PP_0063 at 74.196 kb on - strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.329 kb on + strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.341 kb on + strand at 74.353 kb on + strand at 74.353 kb on + strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.410 kb on + strand at 74.410 kb on + strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.497 kb on + strand at 74.497 kb on + strand at 74.498 kb on - strand at 74.646 kb on + strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.654 kb on - strand, within PP_0064 at 74.679 kb on + strand, within PP_0064 at 74.680 kb on - strand, within PP_0064 at 74.696 kb on + strand, within PP_0064 at 74.985 kb on + strand, within PP_0065 at 75.196 kb on + strand, within PP_0065 at 75.196 kb on + strand, within PP_0065 at 75.209 kb on + strand, within PP_0065
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 72,893 - -2.9 72,893 - -2.3 72,893 - +1.2 72,895 - +0.7 72,917 - -1.2 72,932 - +0.1 72,932 - +0.4 72,969 - +0.1 73,073 - PP_0062 0.26 +1.9 73,073 - PP_0062 0.26 +0.6 73,112 + PP_0062 0.33 +0.8 73,112 + PP_0062 0.33 -0.2 73,113 - PP_0062 0.33 +0.9 73,113 - PP_0062 0.33 +4.3 73,113 - PP_0062 0.33 +0.1 73,113 - PP_0062 0.33 +0.4 73,128 + PP_0062 0.36 -0.4 73,406 + PP_0062 0.86 -0.6 73,406 + PP_0062 0.86 -0.2 73,407 - PP_0062 0.86 +0.2 73,489 + -0.9 73,489 + +1.1 73,490 - +0.9 73,490 - +1.5 73,490 - -2.2 73,512 - +2.5 73,662 - PP_0063 0.15 -0.1 73,746 + +0.5 73,747 - PP_0063 0.25 -0.7 73,858 - PP_0063 0.38 -0.3 73,915 + PP_0063 0.44 +1.5 73,915 + PP_0063 0.44 -1.4 73,915 + PP_0063 0.44 -0.1 73,915 + PP_0063 0.44 +0.9 73,916 - PP_0063 0.44 +2.5 74,082 + PP_0063 0.63 +0.9 74,195 + PP_0063 0.75 -1.1 74,196 - PP_0063 0.76 +0.9 74,275 + PP_0063 0.84 -1.3 74,275 + PP_0063 0.84 -0.1 74,275 + PP_0063 0.84 -0.1 74,276 - PP_0063 0.85 -1.4 74,276 - PP_0063 0.85 -1.3 74,276 - PP_0063 0.85 +2.5 74,276 - PP_0063 0.85 +0.9 74,276 - PP_0063 0.85 -1.3 74,329 + -0.1 74,330 - -0.5 74,330 - -0.3 74,330 - -0.0 74,330 - +2.5 74,341 + +0.9 74,353 + +0.2 74,353 + -0.7 74,354 - +0.9 74,354 - +2.5 74,354 - +0.9 74,410 + +0.4 74,410 + -2.6 74,411 - -0.7 74,411 - -0.6 74,411 - +0.6 74,411 - -0.1 74,411 - +1.1 74,411 - +1.2 74,431 + +0.6 74,431 + +0.1 74,431 + +1.1 74,431 + +0.5 74,431 + +0.4 74,497 + -0.4 74,497 + +1.9 74,498 - -0.5 74,646 + PP_0064 0.43 +0.3 74,647 - PP_0064 0.43 +1.0 74,647 - PP_0064 0.43 -3.2 74,647 - PP_0064 0.43 -1.9 74,654 - PP_0064 0.46 +0.5 74,679 + PP_0064 0.54 -0.1 74,680 - PP_0064 0.54 +0.4 74,696 + PP_0064 0.59 -0.2 74,985 + PP_0065 0.11 -0.8 75,196 + PP_0065 0.26 -1.9 75,196 + PP_0065 0.26 -0.8 75,209 + PP_0065 0.27 -0.9
Or see this region's nucleotide sequence