Strain Fitness in Pseudomonas putida KT2440 around PP_4188

Experiment: 3-Methyl-2-Oxobutanoic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4187 and PP_4188 are separated by 93 nucleotidesPP_4188 and PP_4189 are separated by 42 nucleotides PP_4187: PP_4187 - dihydrolipoyl dehydrogenase, at 4,730,007 to 4,731,443 _4187 PP_4188: PP_4188 - 2-oxoglutarate dehydrogenase dihydrolipoyltranssuccinylase subunit, at 4,731,537 to 4,732,760 _4188 PP_4189: PP_4189 - 2-oxoglutarate decarboxylase, thiamine-requiring E1 subunit, at 4,732,803 to 4,735,634 _4189 Position (kb) 4731 4732 4733Strain fitness (log2 ratio) -3 -2 -1 0 1at 4731.470 kb on - strandat 4731.539 kb on - strandat 4732.782 kb on - strandat 4733.695 kb on + strand, within PP_4189

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-Methyl-2-Oxobutanoic Acid (C)
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4,731,470 - -3.1
4,731,539 - +0.2
4,732,782 - -0.2
4,733,695 + PP_4189 0.31 +0.8

Or see this region's nucleotide sequence