Experiment: 3-Methyl-2-Oxobutanoic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0274 and PP_0275 are separated by 419 nucleotides PP_0275 and PP_0276 overlap by 4 nucleotides PP_0276 and PP_t73 are separated by 287 nucleotides PP_t73 and PP_0277 are separated by 98 nucleotides PP_0277 and PP_5437 are separated by 203 nucleotides
PP_0274: PP_0274 - putative transcriptional regulator containing HTH domain, at 333,257 to 333,730
_0274
PP_0275: PP_0275 - conserved protein of unknown function, at 334,150 to 334,365
_0275
PP_0276: PP_0276 - Transcriptional regulator, Cro/CI family, at 334,362 to 334,685
_0276
PP_t73: PP_t73 - tRNA-Thr, at 334,973 to 335,048
_t73
PP_0277: PP_0277 - conserved exported protein of unknown function, at 335,147 to 335,395
_0277
PP_5437: PP_5437 - protein of unknown function, at 335,599 to 335,781
_5437
Position (kb) 334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 333.522 kb on + strand, within PP_0274 at 333.522 kb on + strand, within PP_0274 at 333.830 kb on - strand at 333.864 kb on - strand at 333.940 kb on + strand at 333.940 kb on + strand at 333.940 kb on + strand at 334.004 kb on + strand at 334.020 kb on + strand at 334.085 kb on - strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.112 kb on + strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.113 kb on - strand at 334.117 kb on + strand at 334.117 kb on + strand at 334.118 kb on - strand at 334.118 kb on - strand at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.219 kb on + strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.220 kb on - strand, within PP_0275 at 334.363 kb on + strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.364 kb on - strand at 334.531 kb on - strand, within PP_0276 at 334.866 kb on + strand at 334.897 kb on + strand at 334.965 kb on - strand at 334.971 kb on + strand at 334.972 kb on - strand at 334.972 kb on - strand at 335.065 kb on - strand at 335.119 kb on - strand at 335.505 kb on + strand at 335.505 kb on + strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.506 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.548 kb on - strand at 335.577 kb on - strand at 335.600 kb on + strand at 335.601 kb on - strand at 335.608 kb on + strand at 335.663 kb on + strand, within PP_5437 at 335.664 kb on - strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.668 kb on + strand, within PP_5437 at 335.669 kb on - strand, within PP_5437 at 335.669 kb on - strand, within PP_5437
Per-strain Table
Position Strand Gene LocusTag Fraction 3-Methyl-2-Oxobutanoic Acid (C) remove 333,522 + PP_0274 0.56 +1.5 333,522 + PP_0274 0.56 +0.6 333,830 - +0.2 333,864 - -1.0 333,940 + -0.4 333,940 + +0.8 333,940 + +0.1 334,004 + -0.0 334,020 + -0.4 334,085 - +0.2 334,112 + -3.2 334,112 + -0.4 334,112 + -1.0 334,112 + +1.0 334,112 + -1.3 334,112 + +0.2 334,112 + +1.0 334,113 - -0.7 334,113 - +0.2 334,113 - +0.1 334,113 - +0.7 334,117 + -0.4 334,117 + -0.5 334,118 - +0.8 334,118 - +1.0 334,219 + PP_0275 0.32 +0.8 334,219 + PP_0275 0.32 +2.0 334,219 + PP_0275 0.32 -1.7 334,219 + PP_0275 0.32 -0.2 334,219 + PP_0275 0.32 -1.0 334,220 - PP_0275 0.32 +0.5 334,220 - PP_0275 0.32 -0.7 334,220 - PP_0275 0.32 +0.4 334,220 - PP_0275 0.32 -0.6 334,363 + +0.1 334,364 - +0.6 334,364 - +0.8 334,364 - -1.3 334,531 - PP_0276 0.52 +1.0 334,866 + -0.5 334,897 + +1.6 334,965 - +1.3 334,971 + +1.1 334,972 - +0.5 334,972 - -0.0 335,065 - -0.3 335,119 - -0.1 335,505 + +0.3 335,505 + +0.7 335,506 - -1.0 335,506 - +0.5 335,506 - +1.6 335,506 - -0.1 335,506 - -0.2 335,548 - +0.2 335,548 - +0.3 335,548 - +0.9 335,577 - +0.6 335,600 + -1.8 335,601 - +0.4 335,608 + -2.4 335,663 + PP_5437 0.35 -0.9 335,664 - PP_5437 0.36 -0.9 335,668 + PP_5437 0.38 -1.9 335,668 + PP_5437 0.38 -0.6 335,668 + PP_5437 0.38 -1.1 335,669 - PP_5437 0.38 +0.0 335,669 - PP_5437 0.38 -0.7
Or see this region's nucleotide sequence