Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23810 and PS417_23815 are separated by 431 nucleotides PS417_23815 and PS417_23820 overlap by 1 nucleotides PS417_23820 and PS417_23825 are separated by 16 nucleotides
PS417_23810: PS417_23810 - acetolactate synthase 3 catalytic subunit, at 5,151,863 to 5,153,587
_23810
PS417_23815: PS417_23815 - pseudouridine synthase, at 5,154,019 to 5,154,348
_23815
PS417_23820: PS417_23820 - hypothetical protein, at 5,154,348 to 5,155,082
_23820
PS417_23825: PS417_23825 - penicillin-binding protein, at 5,155,099 to 5,157,423
_23825
Position (kb) 5154
5155 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5153.067 kb on + strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.068 kb on - strand, within PS417_23810 at 5153.149 kb on - strand, within PS417_23810 at 5153.228 kb on - strand, within PS417_23810 at 5153.228 kb on - strand, within PS417_23810 at 5153.319 kb on + strand, within PS417_23810 at 5153.319 kb on + strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.320 kb on - strand, within PS417_23810 at 5153.487 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.520 kb on + strand at 5153.521 kb on - strand at 5153.524 kb on + strand at 5153.526 kb on + strand at 5153.526 kb on + strand at 5153.527 kb on - strand at 5153.527 kb on - strand at 5153.577 kb on - strand at 5153.600 kb on + strand at 5153.613 kb on + strand at 5153.613 kb on + strand at 5153.640 kb on + strand at 5153.641 kb on - strand at 5153.643 kb on + strand at 5153.643 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.649 kb on + strand at 5153.687 kb on + strand at 5153.775 kb on - strand at 5153.775 kb on - strand at 5154.185 kb on - strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.313 kb on + strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.314 kb on - strand, within PS417_23815 at 5154.528 kb on + strand, within PS417_23820 at 5154.528 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.742 kb on + strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.743 kb on - strand, within PS417_23820 at 5154.870 kb on + strand, within PS417_23820 at 5155.055 kb on + strand at 5155.058 kb on + strand at 5155.059 kb on - strand at 5155.059 kb on - strand at 5155.199 kb on + strand at 5155.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,153,067 + PS417_23810 0.70 -1.9 5,153,068 - PS417_23810 0.70 -1.3 5,153,068 - PS417_23810 0.70 -3.7 5,153,068 - PS417_23810 0.70 -1.7 5,153,149 - PS417_23810 0.75 +0.8 5,153,228 - PS417_23810 0.79 -0.4 5,153,228 - PS417_23810 0.79 -0.8 5,153,319 + PS417_23810 0.84 -2.3 5,153,319 + PS417_23810 0.84 -0.5 5,153,320 - PS417_23810 0.84 -1.2 5,153,320 - PS417_23810 0.84 -0.6 5,153,320 - PS417_23810 0.84 -3.3 5,153,320 - PS417_23810 0.84 -2.3 5,153,487 + -1.0 5,153,520 + -3.5 5,153,520 + -0.1 5,153,520 + -2.8 5,153,521 - +0.1 5,153,524 + -0.2 5,153,526 + -3.0 5,153,526 + -2.6 5,153,527 - -3.3 5,153,527 - -2.3 5,153,577 - -2.4 5,153,600 + +1.8 5,153,613 + -3.9 5,153,613 + -0.7 5,153,640 + +1.9 5,153,641 - +0.6 5,153,643 + -1.3 5,153,643 + -0.1 5,153,649 + +1.5 5,153,649 + -0.3 5,153,649 + -1.1 5,153,687 + -1.3 5,153,775 - +0.4 5,153,775 - +0.5 5,154,185 - PS417_23815 0.50 -1.7 5,154,313 + PS417_23815 0.89 +0.5 5,154,313 + PS417_23815 0.89 -1.4 5,154,313 + PS417_23815 0.89 -0.0 5,154,313 + PS417_23815 0.89 -0.8 5,154,314 - PS417_23815 0.89 +1.0 5,154,314 - PS417_23815 0.89 -1.5 5,154,314 - PS417_23815 0.89 -2.4 5,154,314 - PS417_23815 0.89 -0.2 5,154,528 + PS417_23820 0.24 -0.2 5,154,528 + PS417_23820 0.24 -2.4 5,154,742 + PS417_23820 0.54 -1.7 5,154,742 + PS417_23820 0.54 +0.3 5,154,742 + PS417_23820 0.54 -0.4 5,154,743 - PS417_23820 0.54 -1.0 5,154,743 - PS417_23820 0.54 -1.3 5,154,743 - PS417_23820 0.54 -0.9 5,154,743 - PS417_23820 0.54 +0.3 5,154,870 + PS417_23820 0.71 -2.5 5,155,055 + -1.9 5,155,058 + +2.1 5,155,059 - -0.5 5,155,059 - +0.5 5,155,199 + +0.5 5,155,200 - -0.3
Or see this region's nucleotide sequence