Experiment: benzenesulfonyl fluoride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murC and ddlB overlap by 8 nucleotides ddlB and ftsQ are separated by 1 nucleotides ftsQ and ftsA overlap by 4 nucleotides
b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240
murC
b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153
ddlB
b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985
ftsQ
b0094: ftsA - cell division protein (NCBI), at 103,982 to 105,244
ftsA
Position (kb) 103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 102.254 kb on + strand at 102.265 kb on + strand at 102.265 kb on + strand at 102.295 kb on + strand at 102.446 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.496 kb on + strand, within ddlB at 102.498 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.793 kb on + strand, within ddlB at 102.829 kb on + strand, within ddlB at 102.984 kb on + strand, within ddlB at 103.002 kb on + strand, within ddlB at 103.017 kb on + strand, within ddlB at 103.068 kb on + strand at 103.138 kb on + strand at 103.911 kb on + strand at 103.911 kb on + strand at 103.916 kb on + strand at 103.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzenesulfonyl fluoride remove 102,254 + +0.0 102,265 + +1.0 102,265 + +0.0 102,295 + -1.3 102,446 + ddlB b0092 0.23 -0.7 102,492 + ddlB b0092 0.28 -2.0 102,492 + ddlB b0092 0.28 +0.0 102,496 + ddlB b0092 0.29 -2.3 102,498 + ddlB b0092 0.29 -0.3 102,573 + ddlB b0092 0.37 -0.8 102,573 + ddlB b0092 0.37 -1.7 102,573 + ddlB b0092 0.37 -2.3 102,793 + ddlB b0092 0.61 -3.3 102,829 + ddlB b0092 0.65 -1.5 102,984 + ddlB b0092 0.82 -1.5 103,002 + ddlB b0092 0.83 -1.0 103,017 + ddlB b0092 0.85 -3.0 103,068 + -1.5 103,138 + +0.0 103,911 + +0.3 103,911 + -2.3 103,916 + +1.6 103,916 + +0.0
Or see this region's nucleotide sequence